Formation and repair of O6-methylguanine in recombination hot spots of plant chromosomes

Environmental and Molecular Mutagenesis

Volumn 29, Issue 4, 1997, Pages 394-399

  Baranczewski, Pawel ^a   Nehls, Peter ^b   Rieger, Rigomar ^a   Pich, Uta ^a   Rajewsky, Manfred F ^b   Schubert, Ingo ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b UNIVERSITY HOSPITAL ESSEN   (Germany)

Author keywords

Chromatid type aberrations; Hot spot; Immuno slot blot analysis; O6 methylguanine; Tandem repetitive element

Indexed keywords

6 O METHYLGUANINE; ALKYLATING AGENT; DNA; METHYLNITROSOUREA; MUTAGENIC AGENT; DRUG DERIVATIVE; ENDODEOXYRIBONUCLEASE FOKI; GUANINE; O (6) METHYLGUANINE; O-(6)-METHYLGUANINE; PLANT DNA; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; CHROMATID ABERRATION; CONTROLLED STUDY; DNA ADDUCT; DNA REPAIR; HETEROCHROMATIN; IMMUNOBLOTTING; METAPHASE CHROMOSOME; NONHUMAN; PLANT; TANDEM REPEAT; VICIA FABA; CHROMATID; CHROMOSOME; CHROMOSOME ABERRATION; DRUG EFFECT; GENETIC RECOMBINATION; GENETICS; LEGUME; MEDICINAL PLANT; MERISTEM; METABOLISM; NUCLEOTIDE REPEAT; PLANT ROOT;

ALKYLATING AGENTS; CHROMATIDS; CHROMOSOME ABERRATIONS; CHROMOSOMES; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA REPAIR; DNA, PLANT; FABACEAE; GUANINE; IMMUNOBLOTTING; MERISTEM; METHYLNITROSOUREA; MUTAGENS; PLANT ROOTS; PLANTS, MEDICINAL; RECOMBINATION, GENETIC; REPETITIVE SEQUENCES, NUCLEIC ACID;

EID: 0030612413     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1997)29:4<394::AID-EM8>3.0.CO;2-A     Document Type: Article

References (20)

1. Angelis K, Bríza J, Satava J, Skákal I, Velemínsky J, Vlasák J, Kleibl K, Margison GP (1992): Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. Mutat Res 273:271-280.
2. 6-methylguanine from plant DNA in vivo is accelerated under conditions of clastogenic adaptation. Environ Mol Mutagen 29:400-405.
3. 6-Alkylguanine-DNA alkyltransferase: inhibition of methylation-induced chromosome aberrations and of lesion persistence into later cell cycles. Environ Mol Mutagen 23(Suppl 23):3.
4. 6-alkylguanine-DNA alkyltransferase. Mutat Res 307:67-81.
5. Beranek DT (1990): Distribution of methyl and ethyl adducts following alkylation with monofunctional alkylating agents. Mutat Res 231:11-30.
6. Döbel P, Schubert I, Rieger R (1978): Distribution of heterochromatin in a reconstructed karyotype of Vicia faba as identified by banding- and DNA-late replication patterns. Chromosoma 69:193-209.
7. Fuchs J, Pich U, Meister A, Schubert I (1994): Differentiation of field bean heterochromatin by in situ hybridization with a repeated FokI sequence. Chromosome Res 2:25-28.
8. 6-methylguanine-DNA methyltransferase (MGMT) cDNA in Chinese hamster cells: The role of MGMT in protection against the genotoxic effects of alkylating agents. Carcinogenesis 12:1857-1867.
9. 6-alkylguanine and N-alkylpurines to the formation of sister chromatid exchanges, chromosomal aberrations, and gene mutations: New insights gained from studies of genetically engineered mammalian cell lines. Environ Mol Mutagen 22:283-292.
10. Kato A, Yakura K, Tanifuji S (1984): Sequence analysis of Vicia faba repeated DNA. the FokI repeat element. Nucleic Acids Res 12:6415-6426.
11. 2 chromosomes with BrdUrd in only one of the sister chromatids. Mutat Res 108:301-316.
12. Michaelis A, Schöneich J, Rieger R (1965): Chromosomenaberrationen bei Vicia faba und Ascitestumoren der Maus nach Einwirkung von N-Nitroso-Methylharnstoff. Chromosoma 16:101-123.
13. 6-ethyldeoxyguanosine in DNA exposed to N-ethyl-N-nitrosourea. Cancer Res 40:887-896.
14. Müller R, Rajewsky MF (1981): Antibodies specific for DNA components structurally modified by chemical carcinogens. J Cancer Res Clin Oncol 102:99-113.
15. Nehls P, Adamkiewicz J, Rajewsky MF (1984): Immuno-slot-blot: A highly sensitive immunoassay for the quantitution of carcinogen-modified nucleosides in DNA. J Cancer Res Clin Oncol 108:23-29.
16. Rieger R, Michaelis A, Nicoloff H (1982): Inducible repair processes in plant root tip meristems? "Below-additivity effects" of unequally fractionated clastogen concentrations. Biol Zentralbl 101:125-138.
17. Rogers SO, Bendich AJ (1985): Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69-76.
18. Sambrook J, Fritsch EF, Maniatis T (1989): Using DNA molecules with protruding 5'-phosphate termini as templates, In: "Molecular Cloning: A Laboratory Manual." 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, pp 10.66-10.67.
19. Schubert I, Heindorff K, Rieger R, Michaelis A (1986): Prinzipien der chromosomalen Verteilung induzierter Chromatidenaberrationen bei Vicia faba und deren mögliche biologische Bedeutung. Kulturpflanze 34:21-45.
20. Schubert I, Rieger R, Fuchs J, Pich U (1994): Sequence organization and the mechanism of interstitial deletion clustering in a plant genome (Vicia faba). Mutat Res 325:1-5.