The PerFect(TM) lipid optimizer kit for maximizing lipid-mediated transfection of eukaryotic cells

BioTechniques

Volumn 22, Issue 5, 1997, Pages 982-987

  Griffiths, T ^a   Russell, M ^a   Froning, K ^a   Brown, B D ^b   Scanlon, S M ^b   Almazan, M ^b   Marcil, R ^a   Hoeffler, J P ^a  

^a INVITROGEN CORPORATION   (United States)

^b Genta Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CHO CELL; CONTROLLED STUDY; DNA TRANSFECTION; HELA CELL; LIPID COMPOSITION; MOUSE; NONHUMAN; ANIMAL; BREAST TUMOR; CELL LINE; CELL STRAIN COS1; CYTOMEGALOVIRUS; ESCHERICHIA COLI; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METHODOLOGY; NERVE CELL; PLASMID; PROMOTER REGION;

ANIMALIA; EUKARYOTA;

BETA GALACTOSIDASE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; LIPID;

3T3 CELLS; ANIMALS; BETA-GALACTOSIDASE; BREAST NEOPLASMS; CELL LINE; CHO CELLS; COS CELLS; CRICETINAE; CYTOMEGALOVIRUS; ESCHERICHIA COLI; HELA CELLS; HUMANS; INDICATORS AND REAGENTS; LIPIDS; MICE; NEURONS; PLASMIDS; PROMOTER REGIONS (GENETICS); TRANSFECTION;

EID: 0030612359     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225pf02     Document Type: Article

References (7)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (Eds.). 1990. Introduction of DNA into mammalian cells, p. 9.0.1-9.5.5. In Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
2. Chen, C. and H. Okayama. 1987. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell Biol. 7:2745-2752.
3. Feigner, P.L., T.R. Gadek, M. Holm, R. Roman, H.W. Chan, M. Wenz, J.P. Northrop, G.M. Ringold and M. Danielsen. 1987. Lipofectin: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84:7413-7417.
4. Lopata, M.A., D.W. Cleveland and B. Soliner-Webb. 1984. High level expression of a chloramphenicol acetyltransferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide of glycerol shock treatment. Nucleic Acids Res. 12:5707.
5. Ray, J. and F.H. Gage. 1992. Gene transfer into established and primary fibroblast cell lines: comparison of transfection methods and promoters. BioTechniques 13:598-603.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Zheng, Q. and D.C. Chang. 1991. High efficiency gene transfection by in situ electroporation of cultured cells. Biochim. Biophys. Acta 1088:104-110.