Combined immmunomagnetic cell sorting and ELISPOT assay for the phenotypic characterization of specific antibody-forming cells

Journal of Immunological Methods

Volumn 203, Issue 2, 1997, Pages 193-198

  Lakew, Mekuria ^a,b   Nordström, Inger ^a   Czerkinsky, Cecil ^a,c   Quiding Järbrink, Marianne ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b ADDIS ABABA UNIVERSITY   (Ethiopia)

^c EDOUARD HERRIOT HOSPITAL   (France)

Author keywords

antibody secreting cell; dynabeads; ELISPOT; phenotype

Indexed keywords

DIPHTHERIA VACCINE; PARASITE ANTIBODY; TETANUS TOXOID; IMMUNOGLOBULIN G;

ANTIBODY PRODUCING CELL; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; HUMAN CELL; LEISHMANIA MAJOR; LEISHMANIASIS; PHENOTYPE; PRIORITY JOURNAL; SUBCUTANEOUS DRUG ADMINISTRATION; VACCINATION; ADULT; ANIMAL; ANTIBODY SPECIFICITY; B LYMPHOCYTE; BIOSYNTHESIS; CLASSIFICATION; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; IMMUNOMAGNETIC SEPARATION; IMMUNOPHENOTYPING; METHODOLOGY; MONONUCLEAR CELL; MOUSE; SKIN LEISHMANIASIS;

ADULT; ANIMALS; ANTIBODY SPECIFICITY; ANTIBODY-PRODUCING CELLS; B-LYMPHOCYTE SUBSETS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; IMMUNOMAGNETIC SEPARATION; IMMUNOPHENOTYPING; LEISHMANIA MAJOR; LEISHMANIASIS, CUTANEOUS; LEUKOCYTES, MONONUCLEAR; MICE; TETANUS TOXOID;

EID: 0030612355     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00030-6     Document Type: Article

References (16)

1. Brieva J.A., Roldan E., de la Sen M.L., Rodriguez C. Human in vivo-induced spontaneous IgG-secreting cells from tonsil, blood and bone marrow exhibit different phenotype and functional level of maturation. Immunology. 72:1991;580.
2. Czerkinsky C., Nilsson L.-Å., Nygren H., Ouchterlony Ö., Tarkowski A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Methods. 65:1983;109.
3. Czerkinsky C., Anderson G., Nilsson L.-Å., Eckre H.-P., Klareskog L., Ouchterlony Ö. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Detection of gamma interferon-secreting cells. J. Immunol. Methods. 110:1988;29.
4. Czerkinsky C., Moldoveanu Z., Mestecky J., Nilsson L.-Å., Ouchterlony Ö. A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells. J. Immunol. Methods. 115:1988;31.
5. Czerkinsky, C., Sedgwick, J., 1993. Enzyme-linked immunospot (ELISPOT) assays for detection of specific antibody-secreting cells. In: Masseyeff, R.F., Albert, W.H., Staines, N.A., (Eds.), Methods of Immunological Analysis. VCH Verlagsgesellschaft, Weinheim, p. 504.
6. Elassad A.M.S., Younis S.A., Siddig M., Grayson J., Petersen E., Ghalib W. The significance of blood levels of IgM, IgA, IgG and IgG subclasses in Sudanese visceral leishmaniasis patients. Clin. Exp. Immunol. 95:1994;294.
7. Gyotoku Y., Mori M., Nonaka Y., Nagata Y., Saito Y. Differences of immunoglobulin secreting cells in bone marrow from those in circulation: relevance to their development. Br. J. Haematol. 59:1985;647.
8. Holt P.G., Plozza T.M. Enumeration of antibody-secreting cells by immunoprinting: sequential readout of different antibody isotypes on individual cell monolayers employing the ELISA plaque assay. J. Immunol. Methods. 93:1986;167.
9. Jerne N.K., Nordin A.A. Plaque formation in agar by single antibody-producing cells. Science. 140:1963;405.
10. Jones B.M. Evaluation of CD5 and other differentiation antigens on human immunoglobulin-secreting cells using a combination of immuno-bead rosetting and reverse haemolytic plaque formation. J. Immunol. Methods. 132:1990;119.
11. + B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP. Eur. J. Immunol. 22:1992;3001.
12. Lea T., Vartdal F., Davies C., Ugelstad J. Magnetic monosized polymer particles for fast and specific fractionation of human mononuclear cells. Scand. J. Immunol. 22:1985;207.
13. Möller S.A., Borrebaeck C.A.K. A filter immuno-plaque assay for the detection of antibody-secreting cells in vitro. J. Immunol. Methods. 79:1985;195.
14. Quiding-Järbrink M., Lakew M., Nordström I., Banchereau J., Butcher E., Holmgren J., Czerkinsky C. Human circulating specific antibody-forming cells after systemic and mucosal immunizations: differential homing commitments and cell surface differentiation markers. Eur. J. Immunol. 25:1995;322.
15. Sedgwick J.D., Holt P.G. A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J. Immunol. Methods. 57:1983;301.
16. Tarkowski A., Czerkinsky C., Nilsson L.-Å. Simultaneous induction of rheumatoid factor- and antigen-specific antibody-secreting cells during the secondary immune response in man. Clin. Exp. Immunol. 61:1985;379.