The EMIT® cyclosporine assay: Development of application protocols for the Boehringer Mannheim Hitachi 911 and 917 analyzers

Clinical Biochemistry

Volumn 30, Issue 2, 1997, Pages 155-162

  Dias, Valerian C ^a   LeGatt, Donald F ^a   Yatscoff, Randall W ^a  

^a UNIVERSITY OF ALBERTA HOSPITAL   (Canada)

Author keywords

cyclosporine; enzyme immunoassay; fluorescence polarization immunoassay (FPIA); high performance liquid chromatography (HPLC); method comparison; random access analyzer; therapeutic drug monitoring

Indexed keywords

CYCLOSPORIN; IMMUNOSUPPRESSIVE AGENT;

ANALYTIC METHOD; ANALYZER; ARTICLE; CLINICAL PROTOCOL; DRUG DETERMINATION; DRUG MONITORING; ENZYME IMMUNOASSAY; FLUORESCENCE POLARIZATION IMMUNOASSAY; HEART TRANSPLANTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; KIDNEY TRANSPLANTATION; LIVER TRANSPLANTATION; PRIORITY JOURNAL; BLOOD; ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLOSPORINE; ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE; FLUORESCENCE POLARIZATION IMMUNOASSAY; HEART TRANSPLANTATION; HUMANS; IMMUNOSUPPRESSIVE AGENTS; KIDNEY TRANSPLANTATION; LIVER TRANSPLANTATION; SENSITIVITY AND SPECIFICITY;

EID: 0030611327     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(96)00162-2     Document Type: Article

References (17)

1. Shaw LM, Bowers LD, Demers L, et al. Critical Issues in Cyclosporine Monitoring: Report of the Task Force on Cyclosporine Monitoring [Special Report]. Clin Chem 1987; 33: 1269-88.
2. Kahan BD, Shaw LM, Holt D, Grevel J, Johnson A. Consensus Document: Hawk's Cay Meeting on Therapeutic Drug Monitoring of Cyclosporine. Clin Chem 1990; 36: 1510-6.
3. Shaw LM, Yatscoff RW, Bowers LD, et al. Canadian Consensus Meeting on Cyclosporine Monitoring: Report of the Consensus Panel. Clin Chem 1990; 36: 1841-6.
4. Oellerich M, Armstrong, VW, Kahan B, et al. Lake Louise Consensus conference on Cyclosporin monitoring in Organ Transplantation: Report of the Consensus panel. Ther Drug Monit 1995; 17: 642-54.
5. Dusci LJ, Hackett LP, Chiswell GH, Iliett KF. Comparison of Cyclosporine Measurement in Whole Blood by High-Performance Liquid Chromatography, Monoclonal Fluorescence Polarization Immunoassay and Monoclonal Enzyme-Multiplied Immunoassay. Ther Drug Monit 1992; 14: 327-32.
6. McBride JH, Kim SS, Rodgerson DO, Reyes AF, Ota MK. Measurement of Cyclosporine by Liquid Chromatography and Three Immunoassays in Blood From Liver, Cardiac and Renal Transplant Recipients. Clin Chem 1992; 38: 2300-6.
7. 125I] Radio-immunoassay. Ther Drug Monit 1992; 14: 226-33.
8. Sabaté I, Gràcia S, Diez O, Alía P, Molikner R. Comparison of Cyclosporine Blood Concentrations Measured by Radioimmunoassay and Two Nonisotopic Immunoassays Using Monoclonal Antibodies. Clin Chem 1992; 38: 1187-8.
9. Beresini MH, Davalian D, Alexander S, et al. Evaluation of EMIT® Cyclosporine Assay For Use With Whole Blood. Clin Chem 1993; 39: 2235-41.
10. LeGatt DF, Chooi M, Simpson AI and Yatscoff RW. The EMIT® Cyclosporine Assay - Development of An Application Protocol for the Technicon AXON® System. Clin Biochem 1994; 27(5): 387-94.
11. Armijo JA and Angeles de Cos M. Parent Cyclosporine in Whole Blood by FPIA and EMIT® After Kidney, Heart and Liver Transplantations. Clin Biochem 1994; 27(6): 498-501.
12. Poonkuzhali B, Victoria J, Selvakumar R, et al. Comparison of HPLC and EMIT® Methods of Cyclosporine Assay in Blood After Bone Marrow Transplantation. Ind J Med Res 1995: 102: 39-41.
13. van Straalen JP, Leyte A, Weber JA, Gorgels J and Sanders TB. Evaluation of the Hitachi 911 for Routine Urine Analysis and for Measurement of Various Special Serum Analytes. Eur J Clin Chem Clin Biochem 1995; 33: 315-322.
14. Schumann G, Petersen D, Hoyer PF and Wonigeit K. Monitoring Cyclosporin A Concentrations in Whole Blood: Evaluation of the EMIT® Assay in Comparison with HPLC and RIA. Eur J Clin Chem Clin Biochem 1993; 31: 381-388.
15. Zerrenthin N, Leimenstall G, Kraft G and Niedermayer W, Comparison of Two Assays for Measuring Cyclosporin A Blood Levels. Transplantationsmedizin 1994; 6(1): 10-12.
16. Heller C, Schütz E, Armstrong VW and Oellerich M. Improved EMIT® Assay for Semiautomated Cyclosporine Determination (abstr). Ther Drug Monit 1995; 17: 416.
17. LeGatt DF, Coates JE, Simpson IA, Shalapay CE, Rintoul BJ and Yatscoff RW. A Comparison of Cyclosporine Assays Using Sequential Samples from Selected Transplant Patients. Clin Biochem 1994; 27(1): 43-48.