Cytosolic Ca2+ and protein kinase Cα couple cellular metabolism to membrane K+ permeability in a human biliary cell line

Journal of Clinical Investigation

Volumn 99, Issue 12, 1997, Pages 2890-2897

  Wang, Yu ^a   Roman, Richard ^a   Schlenker, Thorsten ^c   Hannun, Yusuf A ^a   Raymond, John ^b   Fitz, J Gregory ^c  

^a DUKE UNIVERSITY   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

ATP; Cholangiocyte; Ion channel; Liver

Indexed keywords

2,4 DINITROPHENOL; ADENOSINE TRIPHOSPHATE; APAMIN; CALCIUM; CHELERYTHRINE; DEOXYGLUCOSE; EGTAZIC ACID; PHORBOL 13 ACETATE 12 MYRISTATE; POTASSIUM CHANNEL; POTASSIUM ION; PROTEIN KINASE C; CHELATING AGENT; POTASSIUM;

ARTICLE; BILE DUCT; CELL LINE; CELL METABOLISM; CHANNEL GATING; CONTROLLED STUDY; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; POTASSIUM CURRENT; PRIORITY JOURNAL; BILE DUCT CARCINOMA; CELL CULTURE; CELL MEMBRANE PERMEABILITY; CYTOSOL; ELECTRIC CONDUCTIVITY; FLUORESCENCE MICROSCOPY; METABOLISM; PATCH CLAMP; PHYSIOLOGY; ULTRASTRUCTURE;

2,4-DINITROPHENOL; ADENOSINE TRIPHOSPHATE; BILE DUCTS; CALCIUM; CELL MEMBRANE PERMEABILITY; CHELATING AGENTS; CHOLANGIOCARCINOMA; CYTOSOL; DEOXYGLUCOSE; ELECTRIC CONDUCTIVITY; HUMANS; MICROSCOPY, FLUORESCENCE; PATCH-CLAMP TECHNIQUES; POTASSIUM; POTASSIUM CHANNELS; PROTEIN KINASE C; TUMOR CELLS, CULTURED;

EID: 0030610399     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119483     Document Type: Article

References (32)

1. Nathanson, M.H., and J.L. Boyer. 1991. Mechanisms and regulation of bile secretion. Hepatology. 14:551-566.
2. Hertl, M., R.C. Harvey, P.E. Swanson, D.D. West, T.K. Howard, S. Shenoy, and S.M. Strasberg. 1995. Evidence of preservation injury to bile ducts by bile salts in the pig and its prevention by infusions of hydrophobic bile salts. Hepatology. 21:1130-1137.
3. Campion, J.-P., N. Porchet, J.-P. Aubert, A. L'Helgoualc'h, and B. Clement. 1995. UW preservation of cultured human gallbladder epithelial cells: phenotypic alterations and differential mucin gene expression in the presence of bile. Hepatology. 21:223-231.
4. Colonna, J.O., A. Shaked, A. Gomes, S.D. Colquhoun, O. Jurim, S. McDiarmid, J.M. Millis, L. Goldstein, and R.W. Busuttil. 1992. Biliary strictures complicating liver transplantation. Ann. Surg. 216:344-352.
5. Kehrer, J.P., D.P. Jones, J.J. Lemasters, J.L. Farber, and H. Jaeschke. 1990. Mechanisms of hypoxic cell injury. Toxicol. Appl. Pharmacol. 106:165-178.
6. Rosser, B.G., and G. Gores. 1995. Liver cell necrosis: cellular mechanisms and clinical implications. Gastroenterology. 108:252-275.
7. ++, and cytotoxicity in rat hepatocytes. J. Biol. Chem. 267:6654-6663.
8. Hallbrucker, C., M. Ritter, F. Lang, W. Gerok, and D. Haussinger. 1993. Hydroperoxide metabolism in rat liver. Eur. J. Biochem. 211:449-458.
9. Mets, B., R. Hickman, R. Allin, J. van Dyk, and Z. Lotz. 1993. Effect of hypoxia on the hepatic metabolism of lidocaine in the isolated perfused pig liver. Heptaology. 17:668-676.
10. + channels in hepatoma cells through a calcium- and protein kinase C alpha-dependent mechanism. J. Biol Chem. 271:18107-18113.
11. Silberberg, S.D., and C. van Breemen. 1992. A potassium current activated by lemakalim and metabolic inhibition in rabbit mesenteric artery. Pfluegers Arch. Eur. J. Physiol. 420:118-120.
12. Basavappa, S., J.P. Middleton, A. Mangel, J. McGill, J.A. Cohn, and I.G. Fitz. 1993. Cl- and K+ transport in human biliary cell lines. Gastroenterology. 104:1796-1805.
13. Roman, R.M., Y. Wang, and J.G. Fitz. 1996. Regulation of cell volume in a human biliary cell line: Calcium-dependent activation of K+ and Cl-currents. Am. J. Physiol. 271:G239-G248.
14. Knuth, A., H. Gabbert, W. Dippold, O. Klein, W. Sachsse, D. Bitter-Suermann, W. Prellwitz, and K.H. Meyer zum Buschenfelde. 1985. Biliary adenocarcinoma. Characterization of three new human tumor cell lines. J. Hepatol. 1:579-596.
15. McGill, J., S. Basavappa, G.H. Shimokura, J.P. Middleton, and J.G. Fitz. 1994. Adenosine triphosphate activates ion permeabilities in biliary epithelial cells. Gastroenterology. 107:236-243.
16. Dengler, W., J. Schulte, D.P. Berger, R. Mertelsmann, and H.H. Fiebig. 1995. Development of a propidium iodide fluorescence assay for proliferation and cytotoxicity assays. Anti-Cancer Drugs. 6:522-532.
17. Hamill, O.P., A. Marty, E. Neher, B. Sakmann, and F.J. Sigworth. 1981. Improved patch clamp techniques for high resolution recording from cells and cell-free membrane patches. Pfluegers Arch. Eur. J. Physiol. 391:85-100.
18. Fitz, J.G., and A. Sostman. 1994. Nucleotide receptors activate cation, potassium, and chloride currents in a liver cell line. Am. J. Physiol. 266:G544-G553.
19. Lidofsky, S.D., M.H. Xie, A. Sostman, B.F. Scharschmidt, and J.G. Fitz. 1993. Vasopressin increases cytosolic sodium concentration in hepatocytes and activates calcium influx through cation-selective channels. J. Biol. Chem, 268:14632-14636.
20. Chang, D., P.S. Hsieh, and D.C. Dawson. 1988. Calcium: a program in basic for calculating the composition of solutions with specified free concentrations of calcium, magnesium and other divalent cations. Comput. Biol. Med. 18:351-366.
21. Fitz, J.G., A. Sostman, and J.P. Middleton. 1994. Regulation of cation channels in liver cells by intracellular calcium and protein kinase C. Am. J. Physiol. 266:G677-G684.
22. Gettys, T.W., K. Sheriff-Carter, J. Moomaw, I.L. Taylor, and J.R. Raymond. 1994. Characterization and use of crude alpha-subunit preparations for quantitative immunoblotting of G proteins. Anal. Biochem. 220:82-91.
23. Wetsel, W.C., W.A. Khan, I. Merchenthaler, H. Rivera, A.E. Halpern, H.M. Phung, A. Negro-Vilar, and Y.A. Hannun. 1992. Tissue and cellular distribution of the extended family of protein kinase C isoenzymes. J. Cell Biol. 117:121-133.
24. Stabel, S., D. Schaap, and P.J. Parker. 1991. Expression of protein kinase C isotypes using baculovirus vectors. Methods Enzymol. 200:670-673.
25. Kazanietz, M.G., L.B. Areces, A. Bahador, H. Mischak, J. Goodnight, J.F. Mushinski, and P.M. Blumberg. 1993. Characterization of ligand and substrate specificity for the calcium-dependent and calcium-independent protein kinase C isozymes. Mol. Pharmacol. 44:298-307.
26. Tohkin, M., N. Yoshimatsu, and T. Matsubara. 1988. Comparison of the action of epinephrine and a respiratory chain uncoupler 2,4-dinitrophenol on calcium mobilization in isolated hepatocytes and perfused livers. Jpn. J. Pharmacol. 46:61-69.
27. Komaru, T., K.G. Lamping, C.L. Eastham, and K.C. Dellsperger. 1991. Role of ATP-sensitive potassium channels in coronary microvascular autoregulatory responses. Circ. Res. 69:1146-1151.
28. + channels in airway smooth muscle are inhibited by cytoplasmic adenosine triphosphate. Pfluegers Arch. Eur. J. Physiol. 417:517-522.
29. Nichols, C.G., and W.J. Lederer. 1991. Adenosine triphosphate-sensitive potassium channels in the cardiovascular system. Am. J. Physiol. 261:H1675-H1686.
30. Miller, A.L., E. Morales, N.R. LeBlanc, and W.C. Cole. 1993. Metabolic inhibition enhances calcium-activated K+ current in smooth muscle cells or rabbit portal vein. Am. J. Physiol. 265:H2184-H2195.
31. Herbert, J.M. 1990. Chelerythrine is a potent and specific inhibitor of protein kinase C. Biochem. Biophys. Res. Commun. 172:993-999.
32. Worrell, R.T., and R.A. Frizzell. 1991. CaMKII mediates stimulation of chloride conductance by calcium in T84 cells. Am. J. Physiol. 260:C877-C882.