Determination of pK(a) values of the histidine side chains of phosphatidylinositol-specific phospholipase C from Bacillus cereus by NMR spectroscopy and site-directed mutagenesis

Protein Science

Volumn 6, Issue 9, 1997, Pages 1937-1944

  Liu, Tun ^a   Ryan, Margret ^a   Dahlquist, Frederick W ^a   Griffith, O Hayes ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

13C( 1) histidine; 1H 13C HSQC; Histidine pK(a); NMR; Phosphatidylinositol specific phospholipase C; Site directed mutagenesis

Indexed keywords

HISTIDINE; PHOSPHATIDYLINOSITOL; PHOSPHOLIPASE C; PHOSPHATIDYLINOSITOL DIACYLGLYCEROL LYASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS CEREUS; ENZYME ACTIVITY; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH;

BACILLUS CEREUS;

BACILLUS CEREUS; BINDING SITES; HISTIDINE; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PHOSPHATIDYLINOSITOL DIACYLGLYCEROL-LYASE; PHOSPHOLIPASE C;

EID: 0030608855     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060914     Document Type: Article

References (30)

1. Anderson DE, Becktel WJ, Dahlquist FW. 1990. pH-induced denaturation of proteins: A single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme. Biochemistry 29:2403-2408.
2. Berridge MJ. 1993. Inositol trisphosphate and calcium signalling. Nature 361:315-325.
3. Bruzik KS, Tsai M-D. 1994. Toward the mechanism of phosphatidylinositide-specific phospholipase C. Bioorg Med Chem 2:49-72.
4. De Camilli P, Emr SD, McPherson PS, Novick P. 1996. Phosphoinositides as regulators in membrane traffic. Science 271:1533-1539.
5. Englund PT. 1993. The structure and biosynthesis of glycosyl phosphatidylinositol protein anchors. Annu Rev Biochem 62:121-138.
6. Essen L-O, Perisic O, Cheung R, Katan M, Williams RL. 1996. Crystal structure of a mammalian phosphoinositide-specific phospholipase C. Nature 380:595-602.
7. 1-2′-guanylic acid complex: An X-ray study. Nature 299: 27-31.
8. Heinz DW, Ryan M, Bullock TL, Griffith OH. 1995. Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol. EMBO J 14:3855-3863.
9. Heinz DW, Ryan M, Smith MP, Weaver LH, Keana JFW, Griffith OH. 1996. Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with glucosaminyl(α1→6)-D-myo-inositol, an essential fragment of GPI anchors. Biochemistry 35:9496-9504.
10. 1. J Biochem 89:1185-1195.
11. Koke JA, Yang M, Henner DJ, Volwerk JJ, Griffith OH. 1991. High-level expression in Escherichia coli and rapid purification ot phosphatidylinositol-specific phospholipase C from Bacillus cereus and Bacillus thuringiensis. Protein Express Purif 2:51-58.
12. Kraulis PJ. 1991. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr 24:946-950.
13. Leatherbarrow RJ. 1992. GraFit cersion 3.0. Staines, UK: Erithacus Software Ltd.
14. 2 hydrolysis and regulation of phospholipase C isozymes. Curr Opin Cell Biol 7:183-189.
15. Lewis KA, Garigapali VR, Zhou C, Roberts MF. 1993. Substrate requirements of bacterial phosphatidylinositol-specific phospholipase C. Biochemistry 32:8836-8841.
16. Lin G, Bennett F, Tsai M-D. 1990. Phospholipids chiral at phosphorus: Stereo-chemical mechanism of reactions catalyzed by phosphatidylinositol-specific phospholipase C from Bacillus cereus and guinea pig uterus. Biochemistry 29:2747-2757.
17. Markley JL. 1975. Observation of histidine residues in proteins by means of nuclear magnetic resonance spectroscopy. Acc Chem Res 8:70-79.
18. 1 with free recognition and catalytic site: Crystal structure analysis at 1.5 Å resolution. J Mol Biol 222:335-352.
19. 1 and mutant Glu58 A1a. Biochemistry 29:7572-7576.
20. Muchmore DC, McIntosh LP, Russell CB, Anderson DE, Dahlquist FW. 1989. Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance. Methods Enzymol 177:44-73.
21. Richards FM, Wyckoff HW. 1971. Bovine pancreatic ribonuclease. In: Boyer PD, eds. The Enzymes. New York: Academic Press, pp 647-806.
22. 1 acts as base catalyst when the true catalytic base, glutamic acid-58, is replaced by alanine. Biochemistry 29:9064-9072.
23. Thompson JE, Raines RT. 1994. Value of general acid-base catalysis to ribonuclease A. J Am Chem Soc 116:5467-5468.
24. Turner AJ, ed. 1990. In: Molecular and cell biology of membrane proteins. Glycolipid anchors of cell-surface proteins. Chichester. West Sussex, UK: Ellis Horwood Ltd. pp 5-220.
25. Volwerk JJ, Filthuth E, Griffith OH, Jain MK. 1994. Phosphatidylinositol-specific phospholipase C from Bacillus cereus at the lipid-water interface: Interfacial binding, catalysis, and activation. Biochemistry 33:3464-3474.
26. 31P NMR study. Biochemistry 29:8056-8062.
27. Volwerk JJ, Wetherwax PB, Evans LM, Kuppe A, Griffith OH. 1989. Phosphatidylinositol-specific phospholipase C from Bacillus cereus: Improved purification, amino acid composition and amino-terminal sequence. J Cell Biochem 39:315-325.
28. Wider G, Wüthrich K. 1993. A simple experimental scheme using pulsed field gradients for coherence-pathway rejection and solvent suppression in phase-sensitive heteronuclear correlation spectra. J Magn Res Ser B 102:239-241.
29. 15N chemical shift referencing in biomolecular NMR. J Biomol NMR 6:135-140.
30. Witholt B, Boekhout M, Brock M, Kingma J, van Heerikhuizen H, de Leji L. 1976. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal Biochem 74:160-170.