Acetylcholine release from rat brain cortical slices evoked by the fraction P4 of the venom of the spider Phoneutria nigriventer has Ca2+ and temperature independent components

Neuroscience Letters

Volumn 219, Issue 3, 1996, Pages 159-162

  Romano Silva, Marco A ^a   Gomez, Marcus V ^a   Diniz, Carlos R ^a,b   Cordeiro, Marta N ^b   Ribeiro, Angela M ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

Author keywords

Acetylcholine release; Calcium; Low temperature; Phoneutria nigriventer; Sodium; Spider venom

Indexed keywords

CALCIUM CHANNEL; SODIUM CHANNEL; SPIDER VENOM;

ACETYLCHOLINE RELEASE; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN SLICE; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; RAT;

ACETYLCHOLINE; ANIMALS; CALCIUM; CEREBRAL CORTEX; CHEMICAL FRACTIONATION; DOSE-RESPONSE RELATIONSHIP, DRUG; RATS; SPIDER VENOMS; TEMPERATURE; TETRODOTOXIN;

EID: 0030606274     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(96)13190-6     Document Type: Article

References (22)

1. [1] Araujo, D.A.M., Beirao, P.S.L. and Gomez, M.V., The effect of crotoxin on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices, Braz. J. Med. Biol. Res., 25 (1992) 707-711.
2. [2] Araujo, D.A.M., Cordeiro, M.N., Diniz, C.R. and Beirão, P.S.L., Effects of a toxic fraction, PhTx2, from the spider Phoneutria nigriventer on the sodium current, Naunyn-Schmiedeberg's Arch. Pharmacol., 347 (1993) 205-208.
3. [3] Ceccarelli, B., Hurlburt, W.P. and Iezzi, N., Effect of α-latrotoxin on the frog neuromuscuiar junction at low temperature, J. Physiol. (London), 402 (1988) 195-217.
4. [4] Del Castillo, J. and Katz, B., Quantal components of the endplate potential, J. Physiol.(London), 124 (1954) 560-573.
5. [5] Diniz, C.R., Separation of proteins and characterization of active substances in the venom of the Brazilian spiders, An. Acad. Bras. Cienc., 238 (1963) 622-627.
6. [6] Douglas, W.W., Stimulus-secretion coupling: the concept and clues from chromaffin and other cells, Br. J. Pharmacol., 34 (1968) 451-474.
7. [7] Fonnum, F., The distribution of glutamate decarboxylase and aspartate transaminase in subcellular fractions of rat and guinea-pig brain, Biochem. J., 106 (1968) 401-412.
8. [8] Gomez, M.V., Dai, M.E.M. and Diniz, C.R., Effect of scorpion venom, tityustoxin on the release of acetylcholine from incubated slices of rat, J. Neurochem., 20 (1973) 1051-1061.
9. 3H-acetylcholine induced by tityustoxin and potassium in brain cortical slices and myenteric plexus, Neurosci. Lett., 196 (1995) 131-133.
10. [10] Henriques, M.C. and Gomez, M.V., Effect of scorpion venom, tityustoxin, on the uptake of calcium by isolated nerve ending particles from brain, Brain Res., 93 (1975) 182-187.
11. [11] Heuser, J. and Miledi, R., Effects of lanthanum ions on function and structure of frog neuromuscuiar junctions, Proc. R. Soc. London B, 179 (1971) 247-260.
12. [12] Israel, M. and Lesbats, B., Application to mammalian tissue of the chemiluminescent method for detecting acetylcholine, J. Neurochem., 39 (1982) 248-250.
13. [13] Lazarovici, P. and Lelkes, P.I., Pardaxin induces exocytosis in bovine adrenal medullary chromafin cells independent of calcium, J. Pharmacol. Exp. Ther., 263 (1992) 1317-1326.
14. [14] Lucas, S., Spiders in Brazil, Toxicon, 26(1988) 759-772.
15. [15] Nakamura, Y., Nakajima, S. and Grundfest, H., The action of tetrodotoxin on electrogenic components of squid giant axons, J. Gen. Physiol., 48 (1965) 985-996.
16. [16] Petrenko, A.G., Perin, M.S., Davletov, B.A., Ushkaryov, Y.A., Geppert, M. and Sudhof, T.C., Binding of synaptotagmin to the alpha-latrotoxin receptor implicates both in synaptic vesicle exocytosis, Nature, 353 (1991) 65-68.
17. [17] Prado, M.A.M., Moraes-Santos, T., Freitas, R.N., Silva, M.A.R. and Gomez, M.V., The assay of in vitro acetylcholine released from brain slices using choline oxidase chemiluminescent assay after removal of eserine from the medium, J. Neurosci. Methods, 31 (1990) 193-196.
18. [18] Prado, M.A.M., Guatimosim, C., Gomez, M.V., Diniz, C.R., Cordeiro, M.N. and Romano-Silva, M.A., A novel tool for the investigation of glutamate release from rat cerebrocortical synaptosomes: the toxin Tx3-3 from the venom of the spider Phoneutria nigriventer, Biochem. J., 314 (1996) 145-150.
19. [19] Rezende, L. Jr., Cordeiro, M.N., Oliveira, E.B. and Diniz, C.R., Isolation of neurotoxic peptides from the venom of the 'armed spider' Phoneutria nigriventer, Toxicon, 29 (1991) 1225-1233.
20. 2- entry linked to rapid glutamate release: studies using the Phoneutria nigriventer toxin PhTx2 and potassium depolarization, Biochem. J., 296 (1993) 313-319.
21. 2+-independent release of neurotransmitter induced by α-latrotoxin, FEBS Lett., 353 (1994) 315-318.
22. [22] Vassaut, A., Lactate dehydrogenase. UV-method with pyruvate and NADH. In H.U. Bergmeyer (Ed.), Methods of Enzymatic Analysis, Vol. 3, Verlagsgesellschaft, Weinheim, 1987, pp. 118-126.