Incorrect base insertion and prematurely terminated transcripts during T7 RNA polymerase transcription elongation past benzo[a]pyrenediol epoxide-modified DNA

Journal of Molecular Biology

Volumn 264, Issue 2, 1996, Pages 213-219

  Choi, Deok Joon ^a   Roth, Richard B ^a   Liu, Tongming ^a   Geacintov, Nicholas E ^a   Scicchitano, David A ^a  

^a NEW YORK UNIVERSITY   (United States)

Author keywords

Base misincorporation; Benzo a pyrenediol epoxide; DNA adducts; T7 RNA polymerase; Transcription

Indexed keywords

7,8 DIHYDROXYBENZO[A]PYRENE 9,10 OXIDE; BENZO[A]PYRENE DERIVATIVE; CYTOSINE; OLIGODEOXYRIBONUCLEOTIDE; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T7; CONTROLLED STUDY; COVALENT BOND; DNA ADDUCT; DNA REPAIR; DNA REPLICATION; DNA TEMPLATE; GENE INSERTION; NONHUMAN; PRIORITY JOURNAL; STEREOCHEMISTRY; TRANSCRIPTION TERMINATION;

EID: 0030606266     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0635     Document Type: Editorial

References (22)

1. Chen, Y.-H. & Bogenhagen, D. F. (1993). Effects of DNA lesions on transcription elongation by T7 RNA polymerase. J. Biol. Chem. 268, 5849-5855.
2. Choi, D.-J., Marino-Alessandri, D. J., Geacintov, N. E. & Scicchitano, D. A. (1994). Site-specific benzo[a]pyrene diol epoxide-DNA adducts inhibit transcription elongation by bacteriophage T7 RNA polymerase. Biochemistry, 33, 780-787.
3. Conney, A. H. (1982). Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons: G. H. A. Clowes memorial lecture. Cancer Res. 42, 4875-4917.
4. Cosman, M., de los Santos, C., Fiala, R., Hingerty, B. E., Singh, S. B., Ibanez, V., Margulis, L. A., Live, D., Geacintov, N. E., Broyde, S. & Patel, D. J. (1992). Solution conformation of the major adduct between the carcinogen (+)-anti-z-benzo[a]pyrene diol epoxide and DNA. Proc. Natl Acad. Sci. USA, 89, 1914-1918.
5. Cosman, M., de los Santos, C., Fiala, R., Hingerty, B. E., Ibanez, V., Luna, E., Harvey, R., Geacintov, N. E., Broyde, S. & Patel, D. J. (1993). Solution conformation of the (+)-cis-anti-[BP]-dG adduct in a DNA duplex: intercalation of the covalently attached benzo[a]pyrenyl ring into the helix and displacement of the modified deoxyguanosine. Biochemistry, 32, 4146-4155.
6. de los Santos, C., Cosman, M., Hingerty, B. E., Ibanez, V., Margulis, L. A., Geacintov, N. E., Broyde, S. & Patel, D. J. (1992). Influence of benzo[a]pyrene diol epoxide chirality on solution conformation of DNA covalent adducts: the (-)-trans-anti-[BP]-GC adduct structure and comparison with the (+)-trans-anti-[BP]-GC enantiomer. Biochemistry, 31, 5245-5252.
7. Donahue, B., Yin, S., Taylor, J.-S., Reines, D. & Hanawalt, P. C. (1994). Transcript cleavage by RNA polymerase II arrested by a cyclobutane pyrimidine dimer in the DNA template. Proc. Natl Acad. Sci. USA, 91, 8502-8506.
8. Grosshans, C. A. & Cech, T. R. (1991). A hammerhead ribozyme allows synthesis of a new form of the Tetrahymena ribozyme homogeneous in length with a 3′ end blocked for transesterification. Nucl. Acids Res. 19, 3875-3880.
9. Hanawalt, P. C. (1994). Transcription-coupled repair and human disease. Science, 266, 1957-1958.
10. Hanawalt, P. C. & Mellon, I. (1993). Stranded in an active gene. Curr. Biol. 3, 67-69.
11. Hruszkewycz, A. M., Canella, K. A., Peltonen, K., Kotrappa, L. & Dipple, A. (1992). DNA polymerase action on benzo[a]pyrene-DNA adducts. Carcinogenesis, 13, 2347-2352.
12. Jacques, J.-P. & Kolakofsky, D. (1991). Pseudo-templated transcription in prokaryotic and eukaryotic organisms. Genes Dev. 5, 707-713.
13. Kahn, J. D. & Hearst, J. E. (1989). Reversibility of nucleotide incorporation by Escherichia coli RNA polymerase, and its effect on fidelity. J. Mol. Biol. 205, 291-314.
14. Romaniuk, P. J. & Uhlenbeck, O. C. (1983). Joining of RNA molecules with RNA ligase. Methods Enzymol. 100, 52-59.
15. Scicchitano, D. A. & Hanawalt, P. C. (1992). Intragenomic repair heterogeneity of DNA damage. Environ. Health Perspect. 98, 45-51.
16. Shibutani, S., Margulis, L. A., Geacintov, N. E. & Grollman, A. P. (1993). Translesional synthesis on a DNA template containing a single stereoisomer of dG-(+)-or dG-(-)-anti-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene). Biochemistry, 32, 7531-7541.
17. Struhl, K. (1994). Ribonucleases. In Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K., eds), pp. 3.13.1-3.13.3, John Wiley & Sons, Inc., New York.
18. Tabor, S. (1994). RNA ligases. In Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K., eds), pp. 3.15.1-3.15.2, John Wiley & Sons, Inc., New York.
19. Xu, R., Mao, B., Xu, J., Li, B., Birke, S., Swenberg, C. E. & Geacintov, N. E. (1995). Stereochemical-dependent bending in oligonucleotide duplexes induced by site specific covalent benzo[a]pyrene diol epoxide-guanine lesions. Nucl. Acids Res. 23, 2314-2319.
20. Ya, N.-Q., Smirnov, S., Cosman, M., Bhanot, S., Ibanez, V. & Geacintov, N. E. (1994). Thermal stabilities of benzo[a]pyrene diol epoxide-modified oligonucleotide duplexes. Effects of mismatched complementary strands and bulges. In Structural Biology: The State of the Art. Proceedings of the Eighth Conversation, State University of New York, Albany (Sarma, R. H. & Sarma, M. H., eds), pp. 349-366, Adenine Press, Schenectady, New York.
21. Zhou, W. & Doetsch, P. W. (1993). Effects of abasic sites and DNA single-strand breaks on prokaryotic RNA polymerases. Proc. Natl Acad. Sci. USA, 90, 6601-6605.
22. Zhou, W. & Doetsch, P. W. (1994). Transcription bypass or blockage at single-strand breaks on the DNA template strand: effect of different 3′ and 5′ flanking groups on the T7 RNA polymerase elongation complex. Biochemistry, 33, 14926-14934.