Limiting dilution analysis of virus-specific memory B cells by an ELISPOT assay

Journal of Immunological Methods

Volumn 199, Issue 1, 1996, Pages 37-46

  Slifka, Mark K ^a   Ahmed, Rafi ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

ELISA; ELISPOT; Limiting dilution analysis; Lymphocytic choriomeningitis virus; Memory B cell

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNE RESPONSE; LYMPHOCYTIC CHORIOMENINGITIS VIRUS; MEMORY CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; VIRUS INFECTION;

ANIMALS; ANTIBODIES, VIRAL; ANTIBODY SPECIFICITY; B-LYMPHOCYTES; CELL COUNT; CELL SEPARATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGIC MEMORY; LYMPHOCYTIC CHORIOMENINGITIS VIRUS; MICE; MICE, INBRED BALB C;

ANIMALIA; CHORIOMENINGITIS VIRUS; LYMPHOCYTIC CHORIOMENINGITIS VIRUS;

EID: 0030606260     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00146-9     Document Type: Article

References (27)

1. + T helper cell function in vivo: differential requirement for induction of antiviral cytotoxic T-cell and antibody responses. J. Virol. 62, 2102.
2. Ahmed, R. and Gray, D. (1996) Immunological memory and protective immunity: understanding their relation. Science 272, 54.
3. Ahmed, R., Salmi, A., Butler, L.D., Chiller, J.M. and Oldstone, M.B.A. (1984) Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. Role in suppression of cytotoxic T lymphocyte response and viral persistence. J. Exp. Med. 160, 521.
4. Barington, T., Sparholt, S., Juul, L. and Heilmann, C. (1992) A simplification of the enzyme-linked immunospot technique: Increased sensitivity for cells secreting IgG antibodies to Haemophilus influenzae type b capsular polysaccharide. J. Immunol. Methods 156, 191.
5. Berek, C., Berger, A. and Apel, M. (1991) Maturation of the immune responsein germinal centers. Cell 67, 1121.
6. Buchmeier, M.J., Welsh, R.M., Dutko, F.J. and Oldstone, M.B.A. (1980) The virology and immunobiology of lymphocytic choriomeningitis virus infection. Adv. Immunol. 30, 275.
7. Bullock, W.W. and Rittenberg, M.B. (1970) In vitro initiated secondary anti-hapten response. II. Increasing cell avidity for antigen. J. Exp. Med. 132, 926.
8. Byrne, J.A. and Oldstone, M.B.A. (1984) Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo. J. Virol. 51, 682.
9. Hilbert, D.M., Cancro, M.P., Scherle, P.A., Nordan, R.P., Van Snick, J., Gerhard, W. and Rudikoff, S. (1989) T cell derived IL-6 is differentially required for antigen-specific antibody secretion by primary and secondary B cells. J. Immunol. 143, 4019.
10. Jacob, J. and Kelsoe, G. (1992) In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl) acetyl II. A common clonal origin for periarteriolar sheath-associated foci and germinal centers. J. Exp. Med. 176, 679.
11. Jamieson, B.D., Butler, L.D. and Ahmed, R. (1987) Effective clearance of a persistent viral infection requires cooperation between virus-specific Lyt2+ T cells and nonspecific bone marrow-derived cells. J. Virol. 61, 3930.
12. Klinman, N.R. (1972) The mechanism of antigenic stimulation of primary and secondary clonal precursor cells. J. Exp. Med. 136, 241.
13. Kocks, C. and Rajewsky, K. (1989) Stable expression and somatic hypermutation of antibody V regions in B-cell developmental pathways. Ann. Rev. Immunol. 7, 537.
14. Lau, L.L., Jamieson, B.D., Somasundaram, T. and Ahmed, R. (1994) Cytotoxic T-cell memory without antigen. Nature 369, 648.
15. Lehmann-Grube, F., Moskophidis, D. and Lohler, J. (1988) Recovery from acute virus infection. Role of cytotoxic T lymphocytes in the elimination of lymphocytic choriomeningitis virus from spleens of mice. Ann. NY Acad. Sci. 532, 238.
16. MacLennan, I.C., Liu, Y.J., Oldfield, S., Zhang, J. and Lane, P.J. (1990) The evolution of B-cell clones. Curr. Top. Microbiol. Immunol. 159, 37.
17. Miller, J.F.A.P. and Sprent, J. (1971) Cell-to-cell interaction in the immune response. VI. Contribution of thymus-derived cells and antibody-forming precursors to immunological memory. J. Exp. Med. 134, 66.
18. Moskophidis, D. and Lehmann-Grube, F. (1984) The immune response of the mouse to lymphocytic choriomeningitis virus. IV. Enumeration of antibody-producing cells in spleens during acute and persistent infection. J. Immunol. 133, 3366.
19. Pillai, P.S., Scott, D.W., White, D.A. and Corley, R.B. (1984) Major histocompatibility interactions in the T-cell-dependent activation of hapten-binding B cells. Immunobiology 166, 345.
20. Rolink, A. and Melchers, F. (1991) Molecular and cellular origins of B lymphocyte diversity. Cell 66, 1081.
21. Sanders, V.M., Uhr, J.W. and Vitetta, E.S. (1987) Antigen-specific memory and virgin B cells differ in their requirements for conjugation to T cells. Cell. Immunol. 104, 419.
22. Slifka, M.K. and Ahmed, R. (1996) Long-term humoral immunity against viruses: revisiting the issue of plasma cell longevity. Trends Microbiol., in press.
23. Slifka, M.K., Matloubian, M. and Ahmed, R. (1995) Bone marrow is a major site of long-term antibody production after acute viral infection. J. Virol. 69, 1895.
24. Slifka, M.K., Shen, H., Matloubian, M., Jensen, E.R., Miller, J.F. and Ahmed, R. (1996) Antiviral cytotoxic T cell memory by vaccination with recombinant Listeria monocytogenes. J. Virol. 70, 2902.
25. Taswell, C. (1981) Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J. Immunol. 126, 1614.
26. Yefenof, E., Sanders, V.M., Uhr, J.W. and Vitetta, E.S. (1986) In vitro activation of murine antigen-specific memory B cells by a T-dependent antigen. J. Immunol. 137, 85.
27. Zinkernagel, R.M. and Doherty, P.C. (1979) MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv. Immunol. 27, 51.