Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker

Gene

Volumn 181, Issue 1-2, 1996, Pages 13-18

  Sizova, Irina A ^a   Lapina, Tatyana V ^a   Frolova, Olga N ^a   Alexandrova, Nelly N ^a   Akopiants, Konstantin E ^b   Danilenko, Valery N ^b  

^a ST PETERSBURG STATE UNIVERSITY   (Russian Federation)

^b State Res Inst of Genet and S   (Russian Federation)

Author keywords

Aminoglycoside 3' phosphotransferase; Expression; Foreign gene; Gene fusion

Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; CELL NUCLEUS DNA; PAROMOMYCIN; PHOSPHOTRANSFERASE;

ANTIBIOTIC RESISTANCE; ARTICLE; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; DNA FLANKING REGION; DNA SEQUENCE; ENZYME ACTIVITY; GENE EXPRESSION; GENE FUSION; GENE SEQUENCE; GENE STRUCTURE; GENETIC TRANSFORMATION; GREEN ALGA; MARKER GENE; MEIOSIS; MITOSIS; NONHUMAN; PHENOTYPE; PLASMID; PRIORITY JOURNAL; STREPTOMYCES;

ANIMALS; CELL NUCLEUS; CHLAMYDOMONAS REINHARDTII; CODON; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC MARKERS; IMMUNOBLOTTING; KANAMYCIN KINASE; PHENOTYPE; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; STREPTOMYCES; TRANSFORMATION, GENETIC;

ALGAE; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; STREPTOMYCES RIMOSUS;

EID: 0030605237     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00384-8     Document Type: Article

References (22)

1. Beck, E., Luwdig, G., Auerswald, E.A., Reiss, B. and Shaller, H. (1982) Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5, Gene 19, 327-336.
2. Bingham, S.E., Cox, J.C. and Sretm, M.D. (1989) Expression of foreign DNA in Chlamydomonas reinhardtii. FEMS Microbiol. Lett. 65, 77-82.
3. Cabanes-Bastos, E., Day, A.G. and Lichtenstein, C.P. (1989) A sensitive and simple assay for neomycin phosphotransferase II activity in transgenic tissue. Gene 77, 169-176.
4. Danilenko, V.N. and Akopiants, K.E. (1995) Instability of the genome and 'silent' genes of actinomycetes. In: Debabov, V.G., Dudnik, Y.V. and Danilenko, V.N. (Eds.), Proceeding of the Ninth International Simposium of the Biology of Actmomycete, Moscow, 1995, pp. 104-112.
5. Debuchy, R., Purton, S., Rochaix, J.-D. (1989) The argininsuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J. 8, 2803-2809.
6. German, D.C. and Levine R.P. (1965) Cytochrome f and plastocyanin: their sequence in the photocynthetic electron transport chain of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 54, 1665-1669.
7. Hall, L.M., Taylor, K.B., Jones, D.D. (1983) Expression of foreign gene in Chlamydomonas reinhardtii. Gene 124, 75-81.
8. Hasnain, S.E., Manavathu, E.K. and Keung, W.-Ch. (1985) DNA mediated transformation of Chamydomonas reinhardtii cells: use of aminoglycoside-3′-phosphortransferase as a selectable marker. Mol. Cell Biol. 5, 3647-3650.
9. Kertbundit, S., De Greeve, H., Deboeck, F., Van Montagu, M. and Hernalsteens, J.-P. (1991) In vivo random β-glucuronidase gene fusions in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 88, 5212-5216.
10. Kindle K.L. (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 87, 1228-1230.
11. Koncz, C., Martini, N., Mayerhofer, R., Koncz-Kalman, Z., Korber, H., Redei, G.P. and Schell, J. (1989) High-frequency T-DNA mediated gene tagging in plants. Proc. Natl. Acad. Sci. USA 86 (1989) 8467-8471.
12. Lea, M.A., Grasso, S.V., Hu, J. and Seidler, N. (1984) Factors affecting the assay of histone H1 and polylysine by binding of Goomassie Blue G. PLEASE COMPLETE.
13. Merchant, S., Hill, K., Kim, J.H., Thompson, J., Zaitlin, D. and Bogorad, L. (1990) Isolation and characterization of a complementary DNA clone for an algal pre-apoplastocyanin. J. Biol. Chem. 265, 12372-12379.
14. Perkins, D.D. (1953) The detection of linkage in tetrad analysis. Genetics 38, 187.
15. Purrello, M., Balazs, J. (1983) Direct hybridization of labelled DNA to DNA in agarose gels. Anal. Biochem. 128, 393-397.
16. Purton, S. and Rochaix, J.-D. (1995) Characterization of the ARG7 gene of Chlamydomonas reinhardtii and its application to nuclear transformation. Eur. J. Physiol. 30, 141-148.
17. Reiss, B., Sprengel, R., Will, H. and Schaller, H. (1984) A new sensitive method for qualitative and quantative assay of neomycin phosphoptransferase in crude cell extracts. Gene 30, 211-218.
18. Rochaix, J.-P. (1980) Restriction fragments from Chlamydomonas chloroplast DNA. Methods Enzymol. 65, 785-795.
19. Sizova, I.A., Lapina, T.V., Kozlov, A.V. (1995) Analysis of the chromosomal DNA fragment isolated from a hybrid autonomous plasmid of Chlamydomonas reinhardtii. Russian J. Genet. 31, 279-286.
20. Southern, P.J. and Berg, P. (1982) Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1, 327-341.
21. R) in constructed hybrid plasmids of Streptomyces lividans. Antibiot. Med. Biotechnol. (Russian) 5, 552-572.
22. Trieu-Cuot, P., Arthur, M. and Courvalin, P. (1987) Origin, evolution and dissimilation of antibiotic resistance genes. Microbiol. Sci. 4, 263-266.