Facile reduction in the synthesis of phosphorylcholine affinity columns

Tetrahedron Letters

Volumn 37, Issue 44, 1996, Pages 7921-7924

  Martin, Lenore M ^a  

^a UNIVERSITY OF RHODE ISLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHORYLCHOLINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; DRUG SYNTHESIS; REACTION ANALYSIS;

EID: 0030605063     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(96)01797-2     Document Type: Article

References (13)

1. 1. Abbreviations used in this manuscript: ELISA, enzyme-linked immunosorbent assay; PC, phosphorylcholine; DPPC, 4-diazophenylphosphorylcholine; BSA, bovine serum albumin; IgA, immunoglobulin A (alpha heavy chain); BBS, borate-buffered saline; TLC, thin-layer chromatography.
2. 2. Claflin, J. L. J. Immunology 1974, 112, 1747-56.
3. 3. Desaymard, C.; Giusti, A. M.; Scharff, M. D. Molecular Immunology 1984, 21, 961-67.
4. 4. Martin, L. M.; Merrifield, R. B. Synthesis and Characterization of Immunoglobulin Variable Region Heavy and Light Chain Fragments. In Peptides: Chemistry and Biology; Smith, J. A.; Rivier, J. E. Eds.; ESCOM: Leiden, 1992; pp. 849-50.
5. 5. Chesebro, B.; Metzger, H. Biochem. 1972, 11, 766-71.
6. 6. Ram, S.; Ehrenkaufer, R. E. Synthesis 1988, 91-95.
7. 7. Anwer, M. K.; Spatola, A. F. Tetrahedron Lett. 1985, 26, 1381.
8. 8. Sajiki, H. Tetrahedron Lett. 1995, 36, 3465-68.
9. 9. Balczewski, P.; Joule, J. A. Synth. Commun. 1990, 20, 2815-19.
10. 2O/DSS) δ 8.28 (d, 2H), 7.36 (d, 2H), 4.76 (HOD), 4.45 (m, 2H), 3.70 (m, 2H), 3.20 (s, 9H).
11. 11. Rudikoff, S.; Potter, M.; Segal, D. M.; Padlan, E. A.; Davies, D. R. Proc. Nat. Acad. of Sciences U.S.A. 1972, 69, 3689-92.
12. 12. The ELJSA procedure used was recommended by the Scharff laboratory.
13. 13. The maximum binding capacity was never reached in our samples as evidenced by the lack of PC-binding activity in the protein which flowed through the column.