Purification and characterization of constituent androstenedione 15α-hydroxylase (Cytochrome P450(15αAD) from mouse liver

Biochemical Pharmacology

Volumn 52, Issue 6, 1996, Pages 901-910

  Sharma, Mahesh C ^a   Sharma, Meena R ^a   Jeong, Seong Joo ^a   Shapiro, Bernard H ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

androstenedione 15 hydroxylase, murine; cytochrome P450(15 AD), murine hepatic; cytochrome P450, murine isoform; cytochrome P450, tissue specificity, murine; drug metabolism, murine; murine P450 2c; sexual dimorphism, cytochrome P450, murine

Indexed keywords

ANDROSTENEDIONE; ANDROSTENEDIONE 15ALPHA HYDROXYLASE; BENZPHETAMINE; COCAINE; CYTOCHROME P450 ANTIBODY; CYTOCHROME P450 ISOENZYME; ENZYME ANTIBODY; HEXOBARBITAL; MONOCLONAL ANTIBODY; RADIOISOTOPE; STEROID 15ALPHA HYDROXYLASE; STEROID 7ALPHA HYDROXYLASE; TESTOSTERONE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANDROGEN METABOLISM; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEMETHYLATION; DRUG METABOLISM; ENZYME ACTIVITY; ENZYME PURIFICATION; FEMALE; LIVER MICROSOME METABOLISM; LUNG; MOLECULAR WEIGHT; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEX DIFFERENCE; STEREOSPECIFICITY; STRAIN DIFFERENCE;

EID: 0030603962     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/0006-2952(96)00418-2     Document Type: Article

References (46)

1. 1. Gonzalez F, The molecular biology of cytochrome P450s. Pharmacol Rev 40: 243-288, 1989.
2. 2. Ryan DE and Levin W, Purification and characterization of hepatic microsomal cytochrome P-450. Pharmacol Ther 45: 153-239, 1990.
3. 3. Nelson DR, Kamataki T, Waxman DJ, Guengerich FP, Estabrook RW, Feyereisen R, Gonzalez FJ, Coon MJ, Gunsalus IC, Gotoh O, Okuda K and Nebert DW, The P450 super-family: Update on new sequences, gene mapping, accession numbers, early trival names of enzymes, and nomenclature. DNA Cell Biol 12: 1-51, 1993.
4. 4. Shapiro BH, Agrawal AK and Pampori NA, Gender differences in drug metabolism regulated by growth hormone. Int J Biochem Cell Biol 27: 9-20, 1995.
5. 5. Schenkman JB, Steroid metabolism by constitutive cytochromes P450. J Steroid Biochem Mol Biol 43: 1023-1030, 1992.
6. 15α). Purification, regioselectivity, stereospecificity, and sex-dependent expression. J Biol Chem 259: 1265-1271, 1984.
7. 16α). Purification, regioselectivity, stereospecificity, and immunochemical characterization. J Biol Chem 259: 12285-12290, 1984.
8. 2α) from mouse liver. Arch Biochem Biophys 316: 478-484, 1995.
9. 9. Devore K, Harada N and Negishi M, Characterization of testosterone 16α-hydroxylase (I-P-45016α) induced by phenobarbital in mice. Biochemistry 24: 5632-5637, 1985.
10. 16α) of testosterone 16α-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA. Biochemistry 27: 6434-6443, 1988.
11. 15α. J Biol Chem 264: 6465-6471, 1989.
12. 15α as coumarin 7-hydroxylase. Biochemistry 28: 4169-4172, 1989.
13. 13. Itoh S, Satoh M, Abe Y, Hashimoto H, Yanagimoto T and Kamataki T, A novel form of mouse cytochrome P450 3A (Cyp3a-16). Its cDNA cloning and expression in fetal liver. Eur J Biochem 226: 877-882, 1994.
14. 14. Matsunaga T, Watanabe K, Yamamoto I, Negishi M, Gonzalez FJ and Yoshimura H, cDNA cloning and sequence of CYP2C29 encoding P-450 MUT-2, a microsomal aldehyde oxygenase. Biochim Biophys Acta 1184: 299-301, 1994.
15. 15. Freeman JE, Stirling D, Russell AL and Wolf CR, cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1. Biochem J 281: 689-695, 1992.
16. 16. Pampori NA and Shapiro BH, Effects of neonatally administered monosodium glutamate on the sexually dimorphic profiles of circulating growth hormone regulating murine hepatic monooxygenases. Biochem Pharmacol 47: 1221-1229, 1994.
17. 17. MacLeod JN, Sorensen MP and Shapiro BH, Strain independent elevation of hepatic monooxygenase enzymes in female mice. Xenobiotica 17: 1095-1102, 1987.
18. 18. Morgan ET, Rönnholm M and Gustafsson J-Å, Preparation and characterization of microsomal antibodies recognizing unique epitopes on sexually differentiated rat liver cytochrome P-450 isozymes. Biochemistry 26: 4193-4200, 1987.
19. 19. Sharma MC, Jeong S-J, Sharma MR and Shapiro BH, Simultaneous isolation of NADPH-cytochrome P-450 reductase and cytochrome P-450 using tentacle ion-exchange chromatography and interspecies comparison of the reductase activity. Pharmacology 51: 331-340, 1995.
20. 20. Shapiro BH and Szczotka SM, Androgenic repression of hexobarbitone metabolism and action in Cr1:CD-1 (ICR)BR mice. Br J Pharmacol 81: 49-54, 1984.
21. 21. Raucy LJ and Lasker MJ, Isolation of P450 enzymes from human livet. Methods Enzymol 206: 577-587, 1991.
22. 22. Agrawal AK, Pampori NA and Shapiro BH, Thin-layer chromatographic separation of regioselective and stereospecific androgen metabolites. Anal Biochem 224: 455-457, 1995.
23. 23. Cochin J and Axelrod J, Biochemical and pharmacological changes in the rat following chronic administration of morphine, nalorphme and normophine. J Pharmacol Exp Ther 125: 105-110, 1959.
24. 24. Boelsterli UA, Lanzotti A, Goldlin C and Oertle M, Identification of cytochrome P-450IIB1 as a cocaine-bioactivating isoform in rat hepatic microsomes and in cultured rat hepatocytes. Drug Metab Dispos 20: 96-101, 1992.
25. 25. Omura T and Sato R, The carbon-monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2370-2378, 1964.
26. 26. Laemmli UK, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
27. 27. Bradford MM, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976.
28. 28. Matsudaira P, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262: 10035-10038, 1987.
29. 29. Reim D, Hembach P and Speicher DW, Evaluation of the blot cartridge for enhanced gas phase sequencing at maximum sensitivity. In: Techniques in Protein Chemistry (Ed. Angeletti RH), pp. 53-60. Academic Press, New York, 1992.
30. 30. Waxman DJ, Rat hepatic P450IIA and P4501IC subfamily expression using catalytic, immunochemical, and molecular probes. Methods Enzymol 206: 249-267, 1991.
31. 31. Pampori NA, Pampori MK and Shapiro BH, Dilution of the chemiluminescence reagents reduces the background noise on western blots. Biotechniques 18: 588-590, 1995.
32. 19 steroids by five highly purified and reconstituted rat hepatic cytochrome P-450 isozymes. J Biol Chem 258: 8839-8847, 1983.
33. 33. Waxman DJ, Dannan GA and Guengerich FP, Regulation of rat hepatic cytochrome P-450: Age-dependent expression, hormonal imprinting and xenobiotic inducibility of sex-specific isoenzymes. Biochemistry 24: 4409-4417, 1985.
34. 34. Miranda CL, Wang J-L, Henderson MC and Buhler DR, Purification and characterization of hepatic steroid hydroxylases from untreated rainbow trout. Arch Biochem Biophys 268: 227-238, 1989.
35. 35. Honkakoski P and Lang MA, Mouse liver phenobarbital-inducible P450 system: Purification, characterization, and differential inducibility of four cytochrome P450 isozymes from the D2 mouse. Arch Biochem Biophys 273: 42-57, 1989.
36. 36. Sonderfan AJ, Arlotto MP and Parkinson A, Identification of the cytochrome P-450 isozymes responsible for testosterone oxidation in rat lung, kidney and testis: Evidence that cytochrome P-450a (P450IIA1) is the physiologically important testosterone 7α-hydroxylase in rat testis. Endocrinology 125: 857-866, 1989.
37. 37. Villard PH, Seree E, Lacarelle B, Therene-Fenoglio MC, Barra Y, Attolini L, Bruguerole B, Durand A and Catalin J, Effect of cigarette smoke on hepatic and pulmonary cytochromes P450 in mouse: Evidence for CYP2E1 induction in lung. Biochem Biophys Res Commun 202: 1731-1737, 1994.
38. 38. Henderson CJ, Scott AR, Yang CS and Wolf CR, Testosterone-mediated regulation of mouse renal cytochrome P-450 isoenzymes. Biochem J 266: 675-681, 1990.
39. 39. Watanabe K, Narimatsu S, Yamamoto I and Yoshimura H, Oxygenation mechanism in conversion of aldehyde to carboxylic acid catalyzed by P-450 isozyme. J Biol Chem 266: 2709-2711, 1991.
40. 40. Morgan ET, MacGeoch C and Gustafsson J-Å, Hormonal and developmental regulation of expression of the hepatic microsomal steroid 16α-hydroxylase cytochrome P-450 apoprotein in the rat. J Biol Chem 260: 11895-11898, 1985.
41. 15β apoprotein levels in rat liver. Endocrinology 117: 2085-2092, 1985.
42. 42. Guengerich FP, Catalytic selectivity of human cytochrome P450 enzymes: Relevance to drug metabolism and toxicity. Toxicol Lett 70: 133-138, 1994.
43. 15α, and contribution to steroid hydroxylase activities in mouse liver microsomes. Biochem Pharmacol 37: 4778-4780, 1988.
44. 44. Bornheim LM and Correia MA, Purification and characterization of the major hepatic cannabinoid hydroxylase in the mouse: A possible member of the cytochrome P-450IIC subfamily. Mol Pharmacol 40: 228-234, 1991.
45. 45. Bandiera S and Dworschak C, Effects of testosterone and estrogen on hepatic levels of cytochromes P450 2C7 and P450 2C11 in the rat. Arch Biochem Biophys 296: 286-295, 1992.
46. 46. Waxman DJ, Regulation of liver-specific steroid metabolizing cytochromes P450: Cholesterol 7α-hydroxylase, bile acid, 6β-hydroxylase, and growth hormone-responsive steroid hormone hydroxylases. J Steroid Biochem Mol Biol 43: 1055-1072, 1992.