Isolation of 88F actin mutants of Drosophila melanogaster and possible alterations in the mutant actin structures

Journal of Molecular Biology

Volumn 260, Issue 4, 1996, Pages 492-505

  An, HyeSuck ^a,b   Mogami, Kaname ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Pusan National University   (South Korea)

Author keywords

Actin; Drosophila; Mutant; PCR; Protein folding

Indexed keywords

ACTIN; DNA FRAGMENT; HEAT SHOCK PROTEIN; MUSCLE PROTEIN; MUTANT PROTEIN;

ALLELE; ARTICLE; CODON; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; FLIGHT MUSCLE; GENE AMPLIFICATION; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; MUSCLE FIBRIL; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; THORAX; TWO DIMENSIONAL GEL ELECTROPHORESIS;

DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0030602907     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0417     Document Type: Article

References (64)

1. Ball, E., Karlik, C. C., Beall, C. J., Saville, D. L., Sparrow, J. C., Bullard, B. & Fyrberg, E. A. (1987). Arthrin, a myofibrillar protein of insect flight muscle, is an actin-ubiquitin conjugate. Cell, 51, 221-228.
2. Bender, W., Spierer, P. & Hogness, D. S. (1983). Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J. Mol. Biol. 168, 17-33.
3. Benzer, S. (1973). Genetic dissection of behavior. Scient. Am. 229(6), 24-37.
4. Bernstein, S. I., O'Donnell, P. T. & Cripps, R. M. (1993). Molecular genetic analysis of muscle development, structure and function in Drosophila. Int. Rev. Cytol. 143, 63-152.
5. Bertrand, R., Chaussepied, P., Kassab, R., Boyer, M., Roustan, C. & Benyamin, Y. (1988). Cross-linking of the skeletal myosin subfragment 1 heavy chain to the N-terminal actin segment of residues 40-113. Biochemistry, 27, 5728-5736.
6. Bonafé, N., Chaussepied, P., Capony, J. P., Derancourt, J. & Kassab, R. (1993). Photochemical cross-linking of the skeletal myosin head heavy chain to actin subdomain-1 at Arg95 and Arg28. Eur. J. Biochem. 213, 1243-1254.
7. Chou, P. Y. & Fasman, G. D. (1978). Prediction of the secondary structure of proteins from their amino acid sequence. Advan. Enzymol. 47, 45-148.
8. Cripps, R. M., Ball, E., Stark, M., Lawn, A. & Sparrow, J. C. (1994). Recovery of dominant, autosomal flightless mutants of Drosophila melanogaster and identification of a new gene required for normal muscle structure and function. Genetics, 137, 151-164.
9. Drummond, D. R., Hennessey, E, S. & Sparrow, J. C. (1991). Characterisation of missense mutations in the Act88F gene of Drosophila melanogaster. Mol. Gen. Genet. 226, 70-80.
10. Drummond, D. R., Hennessey, E. S. & Sparrow, J. C. (1992). The binding of mutant actins to profilin, ATP and DNase I. Eur. J. Biochem. 209, 171-179.
11. Fyrberg, E. A. & Beal, C. (1990). Genetic approaches to myofibrils form and function in Drosophila. Trends Genet. 6, 126-131.
12. Fyrberg, E. A., Kindle, K.L., Davidson, N. & Sodja, A. (1980). The actin genes of Drosophila: a dispersed multigene family. Cell, 19, 365-378.
13. Fyrberg, E. A., Bond, B. J., Hershey, N. D., Mixter, K. S. & Davidson, N. (1981). The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not. Cell, 24, 107-116.
14. Fyrberg, E. A., Mahaffey, J. W., Bond, B. J. & Davidson, N. (1983). Transcripts of the six Drosophila actin genes accumulate in a stage-and tissue-specific manner. Cell, 33, 115-123.
15. Hennessey, E. S., Drummond, D. R. & Sparrow, J. C. (1993). Molecular genetics of actin function. Biochem. J. 291, 657-671.
16. Hiromi, Y. & Hotta, Y. (1985). Actin gene mutations in Drosophila; heat shock activation in the indirect flight muscles. EMBO J. 4, 1681-1687.
17. Hiromi, Y., Okamoto, H., Gehring, W. J. & Hotta, Y. (1986). Germline transformation with Drosophila mutant actin genes induces constitutive expression of heat shock genes. Cell, 44, 293-301.
18. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W. (1990). Atomic model of the actin filament. Nature, 347, 44-49.
19. Johnson, P. & Blazyk, J. M. (1978). Involvement of an arginine residue of actin in tropomyosin binding. Biochem. Biophys. Res. Commun. 82, 1013-1018.
20. Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F. & Holmes, K. C. (1990). Atomic structure of the actin: DNase I complex. Nature, 347, 37-44.
21. Karlik, C. C. & Fyrberg, E. A. (1985). An insertion within a variably spliced Drosophila tropomyosin gene blocks accumulation of only one encoded isoform. Cell, 41, 57-66.
22. Karlik, C. C., Coutu, M. D. & Fyrberg, E. A. (1984). A nonsense mutation within the act88F actin gene disrupts myofibril formation in Drosophila indirect flight muscles. Cell, 38, 711-719.
23. Karlik, C. C., Saville, D. L. & Fyrberg, E. A. (1987). Two missense alleles of the Drosophila melanogaster act88F actin gene are strongly antimorphic but only weakly induce synthesis of heat shock proteins. Mol. Cell. Biol. 7, 3084-3091.
24. Koana, T. & Hotta, Y. (1978). Isolation and characterization of flightless mutants in Drosophila melanogaster. J. Embryol. Expt. Morphol. 45, 123-143.
25. Krause, M., Wild, M., Rosenzweig, B. & Hirsh, D. (1989). Wild-type and mutant actin genes in Caenorhabditis elegans. J. Mol. Biol. 208, 381-392.
26. Kronert, W. A., O'Donnell, P. T., Fieck, A., Lawn, A., Vigoreaux, J. O., Sparrow, J. C. & Bernstein, S. I. (1995). Defects in the Drosophila myosin rod permit sarcomere assembly but cause flight muscle degeneration. J. Mol. Biol. 249, 111-125.
27. Lewis, E. B. & Bacher, F. (1968). Method of feeding ethyl methane sulfonate (EMS) to Drosophila males. Drosoph. Inf. Serv. 43, 193.
28. Lindsley, D. L. & Zimm, G. G. (1992). The Genome of Drosophila melanogaster, Academic Press, San Diego.
29. Lorenz, M., Popp, D. & Holmes, K. C. (1993). Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm. J. Mol Biol. 234, 826-836.
30. Lorenz, M., Poole, K. J. V., Popp, D., Rosenbaum, G. & Holmes, K. C. (1995). An atomic model of the unregulated thin filament obtained by X-ray fiber diffraction on oriented actin-tropomyosin gels. J. Mol. Biol. 246, 108-119.
31. McCabe, P. C. (1990). Production of single-stranded DNA by asymmetric PCR. In PCR Protocols: A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J., eds), pp. 76-83, Academic Press, San Diego.
32. McLaughlin, P. J., Gooch, J. T., Mannherz, H.-G. & Weeds, A. G. (1993). Structure of gelsolin segment 1-actin complex and the mechanism of filament severing. Nature, 364, 685-692.
33. Milligan, R. A. (1996). Protein-protein interactions in the rigor actomyosin complex. Proc. Natl Acad. Sci. USA, 93, 21-26.
34. Mimura, N. & Asano, A. (1987). Further characterization of a conserved actin-binding 27-kDa fragment of actinogelin and α-actinins and mapping of their binding sites on the actin molecule by chemical cross-linking. J. Biol. Chem. 262, 4717-4723.
35. Mogami, K. & Hotta, Y. (1981). Isolation of Drosophila flightless mutants which affect myofibrillar proteins of indirect flight muscle. Mol. Gen. Genet. 183, 409-417.
36. Mogami, K., Fujita, S. C. & Hotta, Y. (1982). Identification of Drosophila indirect flight muscle myofibrillar proteins by means of two-dimensional electrophoresis. J. Biochem. (Tokyo), 91, 643-650.
37. Moir, A. J. G. & Levine, B. A. (1986). Protein cognitive sites on the surface of actin. A proton NMR study. J. Inorg. Biochem. 28, 271-278.
38. 1HNMR investigation. J. Muscle Res. Cell Motil. 8, 68-69.
39. O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007-4021.
40. Okamoto, H. (1987). Structure and functional analyses of the actin gene, using the indirect flight muscle of Drosophila melanogaster as the in vitro assay system. PhD thesis, University of Tokyo.
41. Okamoto, H., Hiromi, Y., Ishikawa, E., Yamada, T., Isoda, K., Maekawa, H. & Hotta, Y. (1986). Molecular characterization of mutant actin genes which induce heat-shock proteins in Drosophila flight muscles. EMBO J. 5, 589-596.
42. Ondek, B., Hardy, R. W., Baker, E. K., Stamnes, M. A., Shieh, B.-H. & Zuker, C. S. (1992). Genetic dissection of cyclophilin function; saturation mutagenesis of the Drosophila cyclophilin homolog nina A. J. Biol. Chem. 267, 16460-16466.
43. Parker, V. P., Falkenthal, S. & Davidson, N. (1985). Characterization of the myosin light-chain-2 gene of Drosophila melanogaster. Mol. Cell. Biol. 5, 3058-3068.
44. Parker-Thornburg, J. & Bonner, J. J. (1987). Mutations that induce the heat shock response of Drosophila. Cell, 51, 763-772.
45. Peckham, M., Molloy, J. E., Sparrow, J. C. & White, D. C. S. (1990). Physiological properties of the dorsal longitudinal flight muscle and the tergal depressor of the trochanter muscle of Drosophila melanogaster. J. Muscle Res. Cell Motil. 11, 203-215.
46. Rayment, I., Rypniewski, W. R., Schmidt-Bäse, K., Smith, R., Tomchick, D. R., Benning, M. M., Winkelmann, D. A., Wesenberg, G. & Holden, H. M. (1993a). Three-dimensional structure of myosin subfragment-1: a molecular motor. Science, 261, 50-58.
47. Rayment, I., Holden, H. M., Whittaker, M., Yohn, C. B., Lorenz, M., Holmes, K. C. & Milligan, R. A. (1993b). Structure of the actin-myosin complex and its implications for muscle contraction. Science, 261, 58-65.
48. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-491.
49. Sakai, Y., Okamoto, H., Mogami, K., Yamada, T. & Hotta, Y. (1990). Actin with tumor-related mutation is antimorphic in Drosophila muscle: two distinct modes of myofibrillar disruption by antimorphic actins. J. Biochem. (Tokyo), 107, 499-505.
50. Sakai, Y., Okamoto, H., Mogami, K., Matsuo, H. & Hotta, Y. (1991). Heat shock gene activation by mutant actin is independent of myofibril degeneration in Drosophila muscle. J. Biochem. (Tokyo), 109, 111-114.
51. Schröeder, R. R., Manstein, D. J., Jahn, W., Holden, H., Rayment, I., Holmes, K. C. & Spudich, J. A. (1993). Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1. Nature, 364, 171-174.
52. Schutt, C. E., Myslik, J. C., Rozycki, M. D., Goonesekere, N. C. W. & Lindberg, U. (1993). The structure of crystalline profilin-β-actin. Nature, 365, 810-816.
53. Sparrow, J., Reedy, M., Ball, E., Kyrtatas, V., Molloy, J., Durston, J., Hennessey, E. & White, D. (1991). Functional and ultrastructural effects of a missense mutation in the indirect flight muscle-specific actin gene of Drosophila melanogaster. J. Mol. Biol. 222, 963-982.
54. Spudich, J. A. (1987). Dictyostelium discoideum: molecular approaches to cell biology. Methods Cell Biol. 28, 1-516.
55. Sutoh, K., Ando, M., Sutoh, K. & Toyoshima, Y. Y. (1991). Site-directed mutations of Dictyostelium actin: disruption of a negative charge cluster at the N terminus. Proc. Natl Acad. Sci. USA, 88, 7711-7714.
56. Tirion, M. M. & ben-Avraham, D. (1993). Normal mode analysis of G-actin. J. Mol. Biol. 230, 186-195.
57. Tirion, M. M., ben-Avraham, D., Lorenz, M. & Holmes, K. C. (1995). Normal modes as refinement parameters for the F-actin model. Biophys. J. 68, 5-12.
58. Tobin, S. L., Zulauf, E., Sánchez, F., Craig, E. A. & McCarthy, B. J. (1980). Multiple actin-related sequences in the Drosophila melanogaster genome. Cell, 19, 121-131.
59. Toffenetti, J., Mischke, D. & Pardue, M. L. (1987). Isolation and characterization of the gene for myosin light chain two of Drosophila melanogaster. J. Cell Biol. 104, 19-28.
60. Tregear, R. T. (1977). Editor of Insect Flight Muscle. North Holland, Amsterdam.
61. Van Eyk, J. E. & Hodges, R. S. (1991). A synthetic peptide of the N-terminus of actin interacts with myosin. Biochemistry, 30, 11676-11682.
62. Vigoreaux, J. O. & Perry, L. M. (1994). Multiple isoelectric variants of flightin in Drosophila stretch-activated muscles are generated by temporally regulated phosphorylations. J. Muscle Res. Cell Motil. 15, 607-616.
63. Wertman, K. F., Drubin, D. G. & Botstein, D. (1992). Systematic mutational analysis of the yeast ACT1 gene. Genetics, 132, 337-350.
64. Yoshihara, M. & Mogami, K. (1995). A simple and efficient method to screen flight defective mutants. Drosoph. Inf. Serv. 76, 181.