Mutations within a conserved region of vaccinia topoisomerase affect the DNA cleavage-religation equilibrium

Journal of Molecular Biology

Volumn 263, Issue 2, 1996, Pages 181-195

  Petersen, Birgitte Ø ^a   Wittschieben, John ^a   Shuman, Stewart ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

Covalent catalysis; Mutagenesis; Topoisomerase; Transesterification

Indexed keywords

ALANINE; DNA TOPOISOMERASE; LYSINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; DNA ADDUCT; DNA CLEAVAGE; DNA SUPERCOILING; ENZYME KINETICS; GENE MUTATION; GENETIC CONSERVATION; NONHUMAN; PRIORITY JOURNAL; VACCINIA;

EUKARYOTA; VACCINIA;

EID: 0030601823     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0568     Document Type: Article

References (30)

1. Caron, P. R. & Wang, J. C. (1994). Alignment of primary sequences of DNA topoisomerases. Advan. Pharmocol. 29B, 271-297.
2. Champoux, J. J. (1990). Mechanistic aspects of type I topoisomerases. In DNA Topology and Its Biological Effects (Cozzarelli, N. R. & Wang, J. C., eds), pp. 217-242, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Eng, W., Pandit, S. D. & Sternglanz, R. (1989). Mapping the active site tyrosine of eukaryotic DNA topoisomerase I. J. Biol. Chem. 264, 13373-13376.
4. Gupta, M., Zhu, C.-X. & Tse-Dinh, Y.-C. (1994). Mutations of vaccinia virus DNA topoisomerase I that stabilize the cleavage complex. J. Biol. Chem. 269, 573-578.
5. Gupta, M., Fujimori, A. & Pommier, Y. (1995). Eukaryotic DNA topoisomerases I. Biochim. Biophys. Acta, 1262, 1-14.
6. Hanai, R. & Wang, J. C. (1994). Protein footprinting by the combined use of reversible and irreversible lysine modifications. Proc. Natl Acad. Sci. USA, 91, 11904-11908.
7. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. & Pease, L. R. (1989). Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene, 77, 51-59.
8. Klemperer, N. & Traktman, P. (1993). Biochemical analysis of mutant alleles of the vaccinia virus topoisomerase I carrying targeted substitutions in a highly conserved domain. J. Biol. Chem. 268, 15887-15899.
9. Kunkel, T. A., Roberts, J. D. & Zakour, R. A. (1987). Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-403.
10. Lynn, R. M., Bjornsti, M.-A., Caron, P. R. & Wang, J. C. (1989). Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I. Proc. Natl Acad. Sci. USA, 86, 3559-3563.
11. Madden, K. R. & Champoux, J. J. (1992). Overexpression of human topoisomerase I in baby hamster kidney cells: hypersensitivity of clonal isolates to camptothecin. Cancer Res. 52, 525-532.
12. Morham, S. G. & Shuman, S. (1990). Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I. Genes Dev. 4, 515-524.
13. Morham, S. G. & Shuman, S. (1992). Covalent and noncovalent DNA binding by mutants of vaccinia DNA topoisomerase I. J. Biol. Chem. 267, 15984-15992.
14. Sekiguchi, J. & Shuman, S. (1994a). Stimulation of vaccinia topoisomerase I by nucleoside triphosphates. J. Biol. Chem. 269, 29760-29764.
15. Sekiguchi, J. & Shuman, S. (1994b). Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA. Nucl. Acids Res. 22, 5360-5365.
16. Sekiguchi, J. & Shuman, S. (1995). Proteolytic footprinting of vaccinia topoisomerase bound to DNA. J. Biol. Chem. 270, 11636-11645.
17. Sekiguchi, J., Stivers, J. T., Mildvan, A. S. & Shuman, S. (1996). Mechanism of inhibition of vaccinia DNA topoisomerase by novobiocin and coumermycin. J. Biol. Chem. 271, 2313-2322.
18. Shuman, S. (1991a). Site-specific DNA cleavage by vaccinia virus DNA topoisomerase I. J. Biol. Chem. 266, 1796-1803.
19. Shuman, S. (1991b). Site-specific interaction of vaccinia virus topoisomerase I with duplex DNA. J. Biol. Chem. 266, 11372-11279.
20. Shuman, S. (1992a). DNA strand transfer reactions catalyzed by vaccinia topoisomerase I. J. Biol. Chem. 267, 8620-8627.
21. Shuman, S. (1992b). Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I. J. Biol. Chem. 267, 16755-16758.
22. Shuman, S. & Moss, B. (1987). Identification of a vaccinia virus gene encoding a type I DNA topoisomerase. Proc. Natl Acad. Sci. USA, 84, 7478-7482.
23. Shuman, S. & Prescott, J. (1990). Specific DNA cleavage and binding by vaccinia virus DNA topoisomerase I. J. Biol. Chem. 265, 17826-17836.
24. Shuman, S., Golder, M. & Moss, B. (1988). Characterization of vaccinia virus DNA topoisomerase I expressed in Escherichia coli. J. Biol. Chem. 263, 16401-16407.
25. Shuman, S., Kane, E. M. & Morham, S. G. (1989). Mapping the active-site tyrosine of vaccinia virus DNA topoisomerase I. Proc. Natl Acad. Sci. USA, 86, 9793-9797.
26. Stivers, J. T., Shuman, S. & Mildvan, A. S. (1994a). Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the strand cleavage and ligation reactions. Biochemistry, 33, 327-339.
27. Stivers, J. T., Shuman, S. & Mildvan, A. S. (1994b). Vaccinia DNA topoisomerase I: kinetic evidence for general acid-base catalysis and a conformational step. Biochemistry, 33, 15449-15458.
28. Tanizawa, A. & Pommier, Y. (1992). Topoisomerase I alteration in a camptothecin-resistant cell line derived from Chinese hamster DC3F cells in culture. Cancer Res. 52, 1848-1854.
29. Tanizawa, A., Bertrand, R., Kohlhagen, G., Tabuchi, A., Jenkins, J. & Pommier, Y. (1993). Cloning of Chinese hamster DNA topoisomerase I cDNA and identification of a single point mutation responsible for camptothecin resistance. J. Biol. Chem. 268, 25463-25468.
30. Wittschieben, J. & Shuman, S. (1994). Mutational analysis of vaccinia DNA topoisomerase defines amino acid residues essential for covalent catalysis. J. Biol. Chem. 269, 29978-29983.