Direct measurement of the torsional rigidity of single actin filaments

Journal of Molecular Biology

Volumn 263, Issue 2, 1996, Pages 227-236

  Yasuda, Ryohei ^a   Miyata, Hidetake ^a   Kinosita Jr , Kazuhiko ^a  

^a KEIO UNIVERSITY   (Japan)

Author keywords

Divalent cation; Flexural rigidity; Fluorescence; Optical microscope; Optical tweezers

Indexed keywords

CALCIUM ION; F ACTIN; MAGNESIUM;

ACTIN FILAMENT; ACTIN POLYMERIZATION; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CELL MOTILITY; METHODOLOGY; MICROSCOPE; MUSCLE CONTRACTION; NONHUMAN; PRIORITY JOURNAL; RIGIDITY; TORSION;

ANIMALIA;

EID: 0030601791     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0571     Document Type: Article

References (41)

1. ben-Avraham, D. & Tirion, M. M. (1995). Dynamic and elastic properties of F-actin: a normal-modes analysis. Biophys. J. 68, 1231-1245.
2. Bremer, A., Millonig, R. C., Sutterlin, R., Engel, A., Pollard, T. D. & Abei, U. (1992). The structural basis for the intrinsic disorder of the actin filament: the "lateral slipping" model. J. Cell. Biol. 115, 689-703.
3. Egelman, E. H., Francis, N. & DeRosier, D. J. (1982). F-actin is a helix with a random variable twist. Nature, 298, 131-135.
4. Egelman, E. H. & DeRosier, D. J. (1992). Image analysis shows that variations in actin crossover spacings are random, not compensatory. Biophys. J. 63, 1299-1305.
5. Erickson, H. P. (1989). Co-operativity in protein-protein association: the structure and stability of the actin filament. J. Mol. Biol. 206, 465-474.
6. Estes, J. E., Selden, L. A., Kinosian, H. J. & Gershman, L. C. (1992). Tightly-bound divalent cation of actin. J. Muscle Res. Cell Motil. 13, 272-284.
7. Finer, J. T., Simmons, R. M. & Spudich, J. A. (1994). Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature, 368, 113-119.
8. Ford, L. E., Huxley, A. F. & Simmons, R. M. (1981). The relation between stiffness and filament overlap in stimulated frog muscle fibers. J. Physiol. 311, 219-249.
9. Gershman, L. C., Selden, L. A., Kinosian, H. J. & Estes, J. E. (1994). Actin-bound nucleotide/divalent cation interactions. In Actin: Biophysics, Biochemistry, and Cell Biology (Estes, J. E. & Higgins, P. J., eds), pp. 35-49. Plenum Press, New York.
10. Gittes, F., Mickey, B., Nettleton, J. & Howard, J. (1993). Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape. J. Cell Biol. 120, 923-934.
11. Goldman, A. J., Cox, R. G. & Brenner, H. (1967). Slow viscous motion of a sphere parallel to a plane wall-I: motion through a quiescent fluid. Chem. Eng. Sci. 22, 637-651.
12. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W. (1990). Atomic model of the actin filament. Nature, 347, 44-49.
13. Huxley, A. F. (1957). Muscle structure and theories of contraction. Progr. Biophys. Mol. Biol. 7, 255-318.
14. Huxley, A. F. & Simmons, R. M. (1971). Proposed mechanism of force generation in striated muscle. Nature, 232, 533-538.
15. Huxley, H. E. (1969). Mechanism of muscular contraction. Science, 164, 1356-1366.
16. Huxley, H. E., Stewart, A., Sosa, H. & Irving, T. (1994). X-ray diffraction measurements of the extensibility of actin and myosin filaments in contracting muscle. Biophys. J. 67, 2411-2421.
17. Isambert, H., Venier, P., Maggs, A. C., Fattoum, A., Kassab, R., Pantaloni, D. & Carlier, M.-F. (1995). Flexibility of actin filaments derived from thermal fluctuations. J. Biol. Chem. 270, 11437-11444.
18. Ishijima, A., Harada, Y., Kojima, H., Funatsu, T., Higuchi, H. & Yanagida, T. (1994). Single-molecule analysis of the actomyosin motor using nano-manipulation. Biochem. Biophys. Res. Commun. 199, 1057-1063.
19. Ishiwata, S., Kinosita, K. Jr., Yoshimura, H. & Ikegami, A. (1987). Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements. J. Biol. Chem. 262, 8314-8317.
20. Kasai, M. & Oosawa, F. (1969). Behavior of divalent cations and nucleotides bound to F-actin. Biochim. Biophys. Acta. 172, 300-310.
21. Kitazawa, T., Shuman, H. & Somlyo, A. P. (1982). Calcium and magnesium binding to thin and thick filaments in skinned muscle fibers: electron probe analysis. J. Muscle Res. Cell Motil. 3, 437-454.
22. Kojima, H., Ishijima, A. & Yanagida, T. (1994). Direct measurement of stiffness of single actin filaments with and without tropomyosin by in vitro nanomanipulation. Proc. Natl Acad. Sci. USA, 91, 12962-12966.
23. Landau, L. D. & Lifshitz, E. M. (1970). Theory of Elasticity, 2nd English edit., pp. 13-14 and 68-75, Pergamon Press, Oxford.
24. MacKintosh, F. C., Käs, J. & Janmey, P. A. (1995). Elasticity of semiflexible biopolymer networks. Phys. Rev. Letters, 75, 4425-4428.
25. Mihashi, K., Nakabayashi, M., Yoshimura, H. & Ohnuma, H. (1979). Absorption, fluorescence, and linear dichroism spectra of fluorescein mercuric acetate (FMA) bound to F-actin. J. Biochem. 85, 359-366.
26. Miyata, H., Yoshikawa, H., Hakozaki, H., Suzuki, N., Furuno, T., Ikegami, A., Kinosita, K. Jr, Nishizaka, T. & Ishiwata, S. (1995). Mechanical measurements of single actomyosin motor force. Biophys. J. 68, 286s-290s.
27. Nishizaka, T., Yagi, T., Tanaka, Y. & Ishiwata, S. (1993). Right-handed rotation of an actin filament in an in vitro motile system. Nature, 361, 269-271.
28. Orlova, A. & Egelman, E. H. (1993). A conformational change in the actin subunit can change the flexibility of the actin filament. J. Mol. Biol. 232, 334-341.
29. Ott, A., Magnasco, M., Simon, A. & Libchaber, A. (1993). Measurement of persistence length of polymerized actin using fluorescence microscopy. Phys. Rev. E, 48, R1642-R1645.
30. Pollard, T. D. & Cooper, J. A. (1982). Methods to characterize actin filament networks. Methods Enzymol 85, 211-233.
31. Prochniewicz, E., Zhang, Q., Howard, E. C. & Thomas, D. D. (1996). Microsecond rotation dynamics of actin: spectroscopic detection and theoretical simulation. J. Mol. Biol. 255, 446-457.
32. Ray, S., Meyhöfer, E., Milligan, R. A. & Howard, J. (1993). Kinesin follows the microtubule's protofilament axis. J. Cell Biol. 121, 1083-1093.
33. Sawyer, W. H., Woodhouse, A. G., Czarmecki, J. J. & Blatt, E. (1988). Rotational dynamics of actin. Biochemistry, 27, 7733-7740.
34. Suzuki, A., Goll, D. E., Singh, L, Allen, R. E., Robson, R. M. & Stromer, M. H. (1976). Some properties of purified skeletal muscle α-actinin. J. Biol. Chem. 251, 6860-6870.
35. Suzuki, N., Miyata, H., Ishiwata, S. & Kinosita, K. Jr (1996). Preparation of bead-tailed actin filaments: estimation of the torque produced by the sliding force in an in vitro motility assay. Biophys. J. 70, 401-408.
36. Tsuda, Y., Yasutake, H., Ishijima, A. & Yanagida, T. (1996). Torsional rigidity and strength of single actin filaments measured directly by in vitro micromanipulation. Biophys. J. 70, A34.
37. Vale, R. D. & Toyoshima, Y. Y. (1988). Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia. Cell, 52, 459-469.
38. Wakabayashi, K., Sugimoto, Y., Tanaka, H., Ueno, Y., Takezawa, Y. & Amemiya, Y. (1994). X-ray diffraction evidence for the extensibility of actin and myosin filaments during muscle contraction. Biophys. J. 67, 2422-2435.
39. Wang, M. C. & Uhlenbeck, G. E. (1954). On the theory of the Brownian motion II. In Selected Papers on Noise and Stochastic Processes (Wax, N., ed.), pp. 113-132, Dover, New York.
40. Yanagida, T., Nakase, M., Nishiyama, K. & Oosawa, F. (1984). Direct observation of motion of single F-actin filaments in the presence of myosin. Nature, 307, 58-60.
41. Yoshimura, H., Nishio, T., Mihashi, K., Kinosita, K, Jr. & Ikegami, A. (1984). Torsional motion of eosin-labeled F-actin as detected in the time-resolved anisotropy decay of the probe in the sub-millisecond time range. J. Mol. Biol. 179, 453-467.