Reduced cholesterol esterification in CaCo-2 cells by indirect action of pravastatin

Atherosclerosis

Volumn 125, Issue 1, 1996, Pages 63-70

  Reimann, Frank M ^a   Winkelmann, Friederike ^a   Fellermann, Klaus ^a   Stange, Eduard F ^a  

^a UNIVERSITY OF LÜBECK   (Germany)

Author keywords

Acyl CoA cholesteryl acyltransferase; CaCo 2 cell; Cholesterol; HMG CoA reductase inhibitor; LDL; Pravastatin; Tetrazolium salts

Indexed keywords

CHOLESTEROL ACYLTRANSFERASE; CHOLESTEROL ESTER; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE INHIBITOR; LOW DENSITY LIPOPROTEIN CHOLESTEROL; MEVALONOLACTONE; PRAVASTATIN; TETRAZOLIUM;

ARTICLE; ATHEROSCLEROSIS; CANCER CELL CULTURE; CHOLESTEROL ESTERIFICATION; CHOLESTEROL METABOLISM; CHOLESTEROL SYNTHESIS; CHOLESTEROL TRANSPORT; CONTROLLED STUDY; ENZYME ASSAY; HUMAN; HUMAN CELL; INTESTINE CELL; MICROSOME; PRIORITY JOURNAL;

EID: 0030598970     PISSN: 00219150     EISSN: None     Source Type: Journal    
DOI: 10.1016/0021-9150(96)05848-0     Document Type: Article

References (36)

1. [1] Vanhanen H, Kesaniemi YA, Miettinen TA. Pravastatin lowers serum cholesterol, cholesterol-precursor sterols, fecal steroids, and cholesterol absorption in man. Metabolism 1992;41:588.
2. [2] Vanhanen H, Miettinen TA. Pravastatin and lovastatin similarly reduce serum cholesterol and its precursor levels in familial hypercholesterolemia. Eur J Clin Pharmacol 1992;42:127.
3. [3] Owens D, Collins P. Johnson A, Tighe O, Robinson K, Tomkin GH. Hypercholesterolemia: simvastatin and pravastatin alter cholesterol metabolism by different mechanisms. Biochim Biophys Acta 1991;1082:303.
4. [4] Ishida F, Watanabe K, Sato A, Taguchi K, Kakubari K, Kitani K, Kamei K. Comparative effects of simvastatin (MK-733) and pravastatin (CS-514) on hypercholesterolemia induced cholesterol feeding in rabbits. Biochim Biophys Acta 1990:1042:365.
5. [5] Koga T, Shimada Y. Kuroda M, Tsujita Y, Hasegawa K, Yamazaki M. Tissue-selective inhibition of cholesterol synthesis in vivo by pravastatin sodium, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor. Biochim Biophys Acta 1990;1045:115.
6. [6] Keys A, Anderson JT. Grande F. Serum cholesterol response to changes in the diet. II. The effect of cholesterol in the diet. Metabolism 1965;13:759.
7. [7] Sodhi HS, Orchard RC, Agnish ND. Varughese PV, Kudchodkar BJ. Separate pools for endogenous and exogenous cholesterol in rat intestines. Atherosclerosis 1973;17:197.
8. [8] Miettinen TA. Inhibition of cholesterol absorption by HMG-CoA reductase inhibitor. Eur J Clin Pharmacol 1991;40(Suppl 1):519.
9. [9] Ishida F. Sato A, lizuka Y, Kitani K, Sawasaki Y, Kamei T. Effects of MK-733 (simvastatin), an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase. on intestinal acylcoenzyme A:cholesterol acyltransferase activity in rabbits. Biochim Biophys Acta 1989;1004:117.
10. 3H]cholesterol in rabbits. Biochim Biophys Acta 1988;963:35.
11. [11] Norum KR, Lilljeqvist A-C. Helgerud P, Normann ER, Mo A, Selbekk B. Esterification of cholesterol in human small intestine: the importance of acyl-CoA:cholesterol acyltransferase. Eur J Clin Invest 1979;9:55.
12. [12] Bennett Clark S. Tercyak AM. Reduced cholesterol transmucosal transport in rats with inhibited mucosal acyl CoA:cholesterol acyltransferase and normal pancreatic function. J Lipid Res 1984:25:148.
13. [13] Watt SM. Simmonds WJ. The effect of pancreatic diversion on lymphatic absorption and esterification of cholesterol in the rat. J Lipid Res 1981;22:157.
14. [14] Heider JG, Pickens CE. Kelly LA. The role of acyl CoA:cholesterol acyltransferase in cholesterol absorption and its inhibition by 57-18 in the rabbit. J Lipid Res 1983:24:1127.
15. [15] Ishida F. Sato A, Iizuka Y. Kamei T. Inhibition of acyl coenzyme A:cholesterol acyltransferase by 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors. Chem Pharm Bull 1989;37:1635.
16. [16] Kam TP, Albright E, Mathur SN, Field FJ. Effect of lovastatin on acyl-CoA:cholesterol O-acyltransferase (ACAT) activity and the basolateral-membrane secretion of newly synthesized lipids by CaCo-2 cells. Biochem J 1990;272:427.
17. [17] Fellermann K. Reimann FM, Herold G, Stange EF. Mevinolin, a competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase, suppresses enterocyte esterification of exogenous but not endogenous cholesterol. Biochim Biophys Acta 1992;1165:78.
18. [18] Fogh J, Wright WC, Loveless JD. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 1977;58:209.
19. [19] Traber MG, Kayden HJ, Rindler MJ. Polarized secretion of newly synthesized lipoproteins by the Caco-2 human intestinal cell line. J Lipid Res 1987;28:1350.
20. [20] Pinto M. Robine Leon S, Appay MD, Kedinger M, Triadou N, Dussaulx E, Lacroix B, Simon-Assman P, Haffen K. Fogh J, Zweibaum A. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell 1983;47:232.
21. [21] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55.
22. [22] Stange EF, Suckling KE, Dietschy JM. Synthesis and coenzyme A-dependent esterification of cholesterol in rat intestinal epithelium - differences in cellular localization and mechanisms of regulation. J Biol Chem 1983;258:12868.
23. [23] Stange EF, Alavi M, Schneider A. Preclik G, Ditschuneit H. Lipoprotein regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in cultured intestinal mucosa. Biochim Biophys Acta 1980;620:520.
24. [24] Arbogast LY, Rothblat GH. Leslie MH. Cooper RA. Cellular cholesterol ester accumulation induced by free cholesterol-rich lipid dispersions. Proc Natl Acad Sci USA 1976:73:3680.
25. [25] Goldstein JL, Basu SK, Brown MS. Receptor-mediated endocytosis of low-density lipoprotein in cultured cells. Methods Enzymol 1983;98:241.
26. [26] Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartuer FH. Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ. Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76.
27. [27] Herold G. Jungwirth R, Rogler G, Geerling I, Stange EF. Influence of cholesterol supply on cell growth and differentiation in cultured enterocytes (CaCo-2). Digestion 1995;56:57.
28. [28] Reimann FM, Herold G, Schneider A, Stange EF. Compartmentalization of cholesterol metabolism and cellular growth in cultured intestinal crypt cells. Biochim Biophys Acta 1991;1085:315.
29. [29] Tsujita Y, Kuroda M. Shimada Y, Tanzawa K, Arai M, Kaneko I, Tanaka M, Masuda H, Tarumi C, Watanabe Y, Fujii S. CS-514, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase: tissue-selective inhibition of sterol synthesis and hypolipidemic effect on various animal species. Biochim Biophys Acta 1986;877:50.
30. [30] Pan HY, DeVault AR, Wang-lverson D, Ivashkiv E, Swanson BN, Sugerman AA. Comparative pharmacokinetics and pharmacodynamics of pravastatin and lovastatin. J Clin Pharmacol 1990;30:1128.
31. [31] Goodman DS, Deykin D, Shiratori T. The formation of cholesterol esters with rat liver enzymes. J Biol Chem 1964;239:1335.
32. [32] Root C, Smith CD, Winegar DA, Brieaddy LE, Lewis MC. Inhibition of ileal sodium-dependent bile acid transport by 2164U90. J Lipid Res 1995;36:1106.
33. [33] Ziegler K, Stünkel W. Tissue-selective action of pravastatin due to hepatocellular uptake via a sodium-independent bile acid transporter. Biochim Biophys Acta 1992;1139:203.
34. [34] Reimann FM, Herold G, Grosshans I, Rogler G, Fellermann K, Stange EF. Regulation of cholesterol metabolism and low-density lipoprotein binding in human intestinal Caco-2 cells. Digestion 1992;51:10.
35. [35] Slater TF, Sawyer B, Sträuli U. Studies on succinate-tetrazolium reductase systems - III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta 1963;77:383.
36. [36] Stange EF, Dietschy JM. Cholesterol synthesis and low density lipoprotein uptake are regulated independently in rat small intestinal epithelium. Proc Natl Acad Sci USA 1983;80:5739.