Functional domains in protein TrwC of plasmid R388: Dissected DNA strand transferase and DNA helicase activities reconstitute protein function

Journal of Molecular Biology

Volumn 264, Issue 1, 1996, Pages 56-67

  Llosa, Matxalen ^a   Grandoso, Guadalupe ^a   Hernando, Miguel Angel ^a   De La Cruz, Fernando ^a  

^a UNIVERSITY OF CANTABRIA   (Spain)

Author keywords

Bacterial conjugation; IncW plasmid R388; oriT specific relaxase; Protein domains; Rolling circle replication

Indexed keywords

HELICASE; OLIGONUCLEOTIDE; TRANSFERASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CONJUGATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; COVALENT BOND; DNA CLEAVAGE; DNA SUPERCOILING; DNA TRANSFER; ENZYME ACTIVITY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION;

BACTERIA (MICROORGANISMS);

EID: 0030598357     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0623     Document Type: Article

References (38)

1. Abdel-Monem, M., Taucher-Scholz, G. & Klinkert, M. Q. (1983). Identification of Escherichia coli DNA helicase I as the tral gene product of the F sex factor. Proc. Natl Acad. Sci. USA, 80, 4659-4663.
2. Bowater, R. P., Aboul-ela, F. & Lilley, M. J. (1994). Large-scale opening of A+T rich regions within supercoiled DNA molecules is suppressed by salt. Nucl. Acids Res. 22, 2042-2050.
3. Brosh, R. M., Jr & Matson, S. W. (1995). Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction. J. Bacteriol. 177, 5612-5621.
4. Chang, A. C. Y. & Cohen, S. N. (1978). Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156.
5. Chow, W. & Berg, D. E. (1988). Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations. Proc. Natl Acad. Sci. USA, 85, 6468-6472.
6. de la Cruz, F. & Grinsted, J. (1982). Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1. J. Bacteriol. 151, 222-228.
7. George, J. W., Brosh, R. M., Jr & Matson, S. W. (1994). A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization. J. Mol. Biol. 235, 424-435.
8. Grandoso, G., Llosa, M., Zabala, J. C. & de la Cruz, F. (1994). Purification and biochemical characterization of TrwC, the helicase involved in plasmid R388 conjugal DNA transfer. Eur. J. Biochem. 226, 403-412.
9. Grant, S. G. N., Jesee, J., Bloom, F. R. & Hanahan, D. (1990). Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl Acad. Sci. USA, 87, 4645-4649.
10. Hanai, R. & Wang, J. C. (1993). The mechanism of sequence-specific DNA cleavage and strand transfer by φX174 gene A* protein. J. Biol. Chem. 268, 23830-23836.
11. Ilyina, T. V. & Koonin, E. V. (1992). Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eukaryotes and archaebacteria. Nucl. Acids Res. 13, 3279-3285.
12. Kornberg, A. & Baker, T. A. (1992). DNA Replication, 2nd edit., W. H. Freeman & Co., New York.
13. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
14. Lanka, E. & Wilkins, B. M. (1995). DNA processing reactions in bacterial conjugation. Annu. Rev. Biochem. 64, 141-169.
15. Llosa, M., Bolland, S. & de la Cruz, F. (1991). Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46. Mol. Gen. Genet. 226, 473-483.
16. Llosa, M., Bolland, S. & de la Cruz, F. (1994a). Genetic organization of the conjugal DNA processing region of the IncW plasmid R388. J. Mol. Biol. 235, 448-464.
17. Llosa, M., Bolland, S., Grandoso, G. & de la Cruz, F. (1994b). Conjugation-independent, site-specific recombination at the oriT of the IncW plasmid R388 mediated by TrwC. J. Bacteriol. 176, 3210-3217.
18. Llosa, M., Grandoso, G. & de la Cruz, F. (1995). Nicking activity of TrwC directed against the origin of transfer of the IncW plasmid R388. J. Mol. Biol. 246, 54-62.
19. Lohman, T. M. (1992). Escherichia coli DNA helicases: mechanisms of DNA unwinding. Mol. Microbiol. 6, 5-14.
20. Matson, S. W. & Morton, B. S. (1991). Escherichia coli DNA helicase I catalyzes a site- and strand-specific nicking reaction at the F plasmid oriT. J. Biol. Chem. 266, 16232-16237.
21. Mendiola, M. & de la Cruz, F. (1992). IS91 transposase is related to the rolling-circle-type replication proteins of the pUB110 family of plasmids. Nucl. Acids Res. 20, 3521.
22. Nelson, W. C., Howard, M. T., Sherman, J. A. & Matson, S. W. (1995). The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT. J. Biol. Chem. 270, 28374-28380.
23. Pansegrau, W., Schröder, W. & Lanka, E. (1993a). Relaxase (TraI) of IncPα plasmid RP4 catalyzes a site-specific cleaving/joining reaction of single-stranded DNA. Proc. Natl Acad. Sci. USA, 90, 2925-2929.
24. Pansegrau, W., Schoumacher, F., Hohn, B. & Lanka, E. (1993b). Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: analogy to bacterial conjugation. Proc. Natl Acad. Sci. USA, 90, 11538-11542.
25. Reygers, U., Wessel, R., Muller, H. & Hoffmann-Berling, H. (1991). Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor. EMBO J. 10, 2689-2694.
26. Rosenberg, A. H., Lade, B. N., Chui, D.-S, Lin, S.-W, Dunn, J. J. & Studier, F. W. (1987). Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene, 56, 125-135.
27. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual 2nd edit., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
28. Scherzinger, E., Kruft, V. & Otto, S. (1993). Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA-cleaving/DNA-joining activity. Eur. J. Biochem. 217, 929-938.
29. Sherman, J. A. & Matson, S. W. (1994). Escherichia coli DNA helicase I catalyzes a sequence-specific cleavage/ligation reaction at the F plasmid origin of transfer. J. Biol. Chem. 269, 26220-26226.
30. Studier, F. W. & Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
31. Trask, D. K., DiDonato, J. A. & Muller, M. T. (1984). Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II. EMBO J. 3, 671-676.
32. Traxler, B. A. & Minkley, E. G., Jr (1988). Evidence that DNA helicase I and oriT site-specific nicking are both functions of the F TraI protein. J. Mol. Biol. 204, 205-209.
33. Vieira, J. & Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19, 259-268.
34. Wessel, R., Müller, H. & Hoffmann-Berling, H. (1990). Electron microscopy of DNA helicase I complexes in the act of strand separation. Eur. J. Biochem. 189, 277-285.
35. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119.
36. Zhang, S. & Meyer, R. J. (1995). Localized denaturation of oriT DNA within relaxosomes of the broad-host-range plasmid R1162. Mol. Microbiol. 17, 727-735.
37. Ziegelin, G., Fürste, J. P. & Lanka, E. (1989). TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin. J. Biol. Chem. 264, 11989-11994.
38. Ziegelin, G., Pansegrau, W., Lurz, R. & Lanka, E. (1992). TraK protein of conjugative plasmid RP4 forms a specialized nucleoprotein complex with the transfer origin. J. Biol. Chem. 267, 17279-17286.