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Volumn 6, Issue 20, 1996, Pages 2467-2472

Novel, selective mechanism-based inhibitors of the herpes proteases

Author keywords

[No Author keywords available]

Indexed keywords

ANTIVIRUS AGENT; CHYMOTRYPSIN; ELASTASE; IMIDAZOLE DERIVATIVE; OXAZOLE DERIVATIVE; PROTEINASE; PROTEINASE INHIBITOR; SPIRO COMPOUND; TRYPSIN;

EID: 0030598180     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(96)00456-8     Document Type: Article
Times cited : (62)

References (17)
  • 7
    • 85030277095 scopus 로고    scopus 로고
    • note
    • 16,17 or HCMV protease catalytic domain for 15 min in 25 uL buffer (50 mM sodium phosphate, 150 mM sodium chloride, 0.001mM EDTA, 0.01% PEG 3400; pH 8.0) then adding 250 μM R-site 14-mer peptide substrate, Ac-HTYLQASEKFKMWG and incubating at 27°C for 60 mins. The reaction was quenched with 40 μL 5% trifluoroacetic acid solution and the extent of proteolysis examined by quantifying the amounts of the substrate peptide and of the N-terminal and C-terminal cleavage fragments by HPLC analysis. The percentage cleavage was calculated and expressed as a fraction of the value obtained for an uninhibited control sample. Compounds 16-19 were assayed in a quenched fluorescence peptidolytic assay (A. Patel, unpublished results).
  • 8
    • 85030276747 scopus 로고    scopus 로고
    • note
    • 8. Mass spectra analysis was carried out by reverse phase HPLC electrospray on a Finnigan TSQ700 and post source decay MS/MS on a Micromass TOF Spec E.
  • 11
    • 85030267937 scopus 로고    scopus 로고
    • note
    • 11. All compounds are racemic and gave satisfactory spectroscopic and analytical date.
  • 13
    • 85030272176 scopus 로고    scopus 로고
    • note
    • 3):-δ 3.5 (s, 3H); 7.3 (s, 1H); 7.5 (m, 6H); 8.2 (dd, J = 7 & 2 Hz, 2H); 8.25 (dd, J = 7 & 2 Hz, 2H).
  • 17
    • 85030280325 scopus 로고    scopus 로고
    • WO Patent Application 95/06055 ( to SmithKline Beecham), 1995
    • 17. DiLella, A. G.; Debouck, C. M., WO Patent Application 95/06055 ( to SmithKline Beecham), 1995.
    • DiLella, A.G.1    Debouck, C.M.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.