Identification of a gene, pilF, required for type 4 fimbrial biogenesis and twitching motility in Pseudomonas aeruginosa

Gene

Volumn 180, Issue 1-2, 1996, Pages 49-56

  Watson, Alison A ^a   Alm, Richard A ^a   Mattick, John S ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Fimbrial biogenesis; pilF; Pseudomonas aeruginosa; Twitching motility

Indexed keywords

GENE PRODUCT;

AMINO ACID SEQUENCE; ARTICLE; BACTERIOPHAGE; BACTERIUM ADHERENCE; BIOGENESIS; CELL MOTILITY; CONTROLLED STUDY; FIMBRIA; GENE LOCATION; GENE MAPPING; KARYOTYPE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PSEUDOMONAS AERUGINOSA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GENETIC COMPLEMENTATION TEST; GENOME, BACTERIAL; MOLECULAR SEQUENCE DATA; MOVEMENT; MUTATION; PSEUDOMONAS AERUGINOSA; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE; PILA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0030597332     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00403-9     Document Type: Article

References (43)

1. Alm, R.A. and Mattick, J.S. (1995) Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa whose product possesses a prepilin-like leader sequence. Mol. Microbiol. 16, 485-496.
2. Alm, R.A. and Mattick, J.S. (1996) Identification of two genes with prepilin-like leader sequences required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa. J. Bacteriol. 178, 3809-3817.
3. Alm, R.A., Bodero, A.J., Free, P. and Mattick, J.S. (1996) Characterization of a novel gene, pilZ, absolutely required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa. J. Bacteriol. 178, 46-53.
4. Beard, M.K.M., Mattick, J.S., Moore, L.J., Mott, M.R., Marrs, C.F. and Egerton, J.R. (1990) Morphogenetic expression of Moraxella bovis fimbriae (pili) in Pseudomonas aeruginosa. J. Bacteriol. 172, 2601-2607.
5. Boyd, J.M., Koga, T. and Lory, S. (1994) Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa. Mol. Gen. Genet. 243, 565-574.
6. Bradley, D.E. (1972a) Evidence for the retraction of Pseudomonas aeruginosa RNA phage pili. Biochem. Biophys. Res. Commun. 47, 142-149.
7. Bradley, D.E. (1972b) Shortening of Pseudomonas aeruginosa pili after RNA-phage adsorption. J. Gen. Microbiol. 72, 303-319.
8. Bradley, D.E. (1973) A pilus dependent Pseudomonas aeruginosa bacteriophage with a long non-retractile tail. Virol. 51, 489-492.
9. Bradley, D.E. (1980) A function of Pseudomonas aeruginosa PAO pili: twitching motility. Can. J. Microbiol. 26. 146-154.
10. Brunschwig, E. and Darzins, A. (1992) A two-component T7 system for the overexpression of genes in Pseudomonas aeruginosa. Gene 111, 35-41.
11. Dalrymple, B. and Mattick, J.S. (1987) An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins. J. Mol. Evol. 25, 261-269.
12. Darzins, A. (1993) The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY. J. Bacteriol. 175, 5934-5944.
13. Darzins, A. (1994) Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosythesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus. Mol. Microbiol. 11, 137-153.
14. Darzins, A. (1995) The Pseudomonas aeruginosa pilK gene encodes a chemotactic methyltransferase (CheR) homologue that is translationally regulated. Mol. Microbiol. 15, 703-717.
15. Fleischman, R.D., Adams, M.D., White, O., Clayton, R.A., Kirkness, E.W., Kerlavage, A.R., Bult, C.J., Tomb, J.-F., Dougherty, B.A., Merrick, J.M., McKenney, K., Sutton, G., FitzHugh, W., Fields, C., Gocayne, J.D., Scott, J., Shirley, R., Liu, L.-I., Glodek, A., Kelley, J.M., Weidman, J.F., Phillips, C.A., Spriggs, T., Hedblom, E., Cotton, M.D., Utterback, T.R, Hanna, M.C., Nguyen, D.T., Saudek, D.M., Brandon, R.C., Fine, L.D., Fritchman, J.L., Fuhrmann, J.L., Geoghagen, N.S.M., Gnehm, C.L., McDonald, L.A., Small, K.V., Fraser, C.M., Smith, H.O. and Venter, J.C. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496-512.
16. Henrichsen, J. (1983) Twitching motility. Ann. Rev. Microbiol. 37, 81-93.
17. Higgins, D.G. and Sharp, P.M. (1989) Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5, 151-153.
18. Hobbs, M., Collie, E.S.R, Free, P.D., Livingston, S.P. and Mattick, J.S. (1993) PilS and PilR, a two-component transcriptional regulatory system controlling transcription of type 4 fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 7. 669-682.
19. Holloway, B.W., Romling, U. and Tummler, B. (1994) Genomic mapping of Pseudomonas aeruginosa PAO. Microbiology 140, 2907-2929.
20. Hoshino, T. and Kageyama, M. (1980) Purification and properties of a binding protein for branched-chain amino acids in Pseudomonas aeruginosa. J. Bacteriol. 141, 1055-1063.
21. Hoyne, P.A., Haas, R., Meyer, T.F., Davies, J.K. and Elleman, T.C. (1992) Production of Neisseria gonorrhoeas pili (fimbriae) in Pseudomonas aeruginosa. J. Bacteriol. 174, 7321-7327.
22. Ishimoto, K.S. and Lory, S. (1992) Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene. J. Bacteriol. 174, 3514-3521.
23. Koga, T., Ishimoto, K. and Lory, S. (1993) Genetic and functional characterization of the gene cluster specifying expression of Pseudomonas aeruginosa pili. Infect. Immun. 61, 1371-1377.
24. Lee, K.K., Sheth, H.B., Y, W.W., Sherburne, R., Paranchych, W., Hodges, R.S., Lingwood, C.A., Krivan, H. and Irvin, R.T. (1994) The binding of Pseudomonas aeruginosa pili to glycosphingolipids is a tip-associated event involving the C-terminal region of the structural pilin subunit. Mol. Microbiol. 11, 705-713.
25. Martin, P.R., Hobbs, M., Free, P.D., Jeske, Y. and Mattick, J.S. (1993) Characterization of pilQ, a new gene required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 9. 857-868.
26. Martin, P.R., Watson, A., McCaul, T. and Mattick, J.S. (1995) Characterisation of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 16, 497-508.
27. Mattick, J.S. and Alm, R.A. (1995) Common architecture of type 4 fimbriae and complexes involved in macromolecular traffic. Trends Microbiol. 3, 411-413.
28. Mattick, J.S., Bills, M.M., Anderson, B.J., Dalrymple, B., Mott, M.R. and Egerton, J.R. (1987) Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa. J. Bacteriol. 169, 33-41.
29. Nunn, D., Bergman, S. and Lory, S. (1990) Products of three accessory genes, pilB, pilC and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J. Bacteriol. 172, 2911-2919.
30. Pasloske, B.L., Finlay, B.B. and Paranchych, W. (1985) Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene. FEBS Lett. 183, 408-412.
31. Pepe, C.M. and Strom, M.S. (1995) Type IV prepilin leader peptidase/methyltransferase and pilus biogenesis genes from Aeromonas hydrophila. Genbank accession number U20255.
32. Ratnaningsih, E., Dharmsthiti, S., Krishnapillai, V., Morgan, A., Sinclair, M. and Holloway, B.W. (1990) A combined physical and genetic map of Pseudomonas aeruginosa PAO. J. Gen. Microbiol. 136, 2351-2357.
33. Russell, M.A. and Darzins, A. (1994) The pilE gene product of Pseudomonas aeruginosa, required for pilus biogenesis, shares amino acid sequence identity with the N-termini of type 4 prepilin proteins. Mol. Microbiol. 13, 973-985.
34. Sheth, H.B., Lee, K.K., Wong, W.Y., Srivastava, G., Hindsgaul, O., Hodges, R.S., Paranchych, W. and Iran, R.T. (1994) The pili of Pseudomonas aeruginosa strains PAK and PAO bind specifically to the carbohydrate sequence βGa1NAc(1-4)βGa1 found in glycosphingolipids asialo-GM1 and asialo-GM2. Mol. Microbiol. 11, 715-723.
35. Strom, M.S., Nunn, D.N. and Lory, S. (1993) A single bifunctional enzyme. PilD, catalyzes cleavage and N-methylation of proteins belonging to the type IV pilin family. Proc. Natl. Acad. Sci. USA 90, 2404-2408.
36. Sundin. G.W., Shankar, S., Chugani, S.A., Chopade, B.A., Kavanaugh-Black, A. and Chakrabarty, A.M. (1996) Mol. Microbiol. 20, 965-979.
37. Tennent J. and Mattick, J.S. (1994) Type 4 Fimbriae. Fimbriae: Aspects Of Adhesion, Genetics, Biogenesis and Vaccines. CRC Press Inc., Boca Raton, Florida, USA, pp. 127-146.
38. Watson, A.A., Mattick, J.S. and Alm, R.A. (1996) Functional expression of heterologous type 4 fimbriae in Pseudomonas aeruginosa. Gene 175, 143-150.
39. West, S.E.H. and Iglewski, B.H. (1988) Codon usage in Pseudomonas aeruginosa. Nucl. Acids Res. 16, 9323-9334.
40. Whitchurch, C.B. and Mattick, J.S. (1994) Characterisation of a gene pilU required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa. Mol. Microbiol. 13, 1079-1091.
41. Whitchurch, C.B., Hobbs, M., Livingston, S.P., Krishnapillai, V. and Mattick, J.S. (1991) Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria. Gene 101, 33-44.
42. Whitchurch, C.B., Alm, R.A. and Mattick, J.S. (1996) The alginate regulator AlgR and an associated sensor FimS are required for twitching motility in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 93, 9839-9843.
43. Wu, S.S. and Kaiser, D. (1995) Genetic and functional evidence that Type IV pili are required for social gliding motility in Myxococcus xanthus. Mol. Microbiol. 18, 547-558.