Expression of recombinant human granulocyte colony-stimulating factor in CHO dhfr- cells: New insights into the in vitro amplification expression system

Gene

Volumn 180, Issue 1-2, 1996, Pages 145-150

  Monaco, Lucia ^a   Tagliabue, Roberta ^a   Giovanazzi, Serenella ^a   Bragonzi, Alessandra ^a   Soria, Marco R ^a  

^a SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

CHO cells; G418; Gene dosage effect; Gene expression; Methotrexate; Tandem ligation; Transfection

Indexed keywords

DIHYDROFOLATE REDUCTASE; NEOMYCIN; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; CHO CELL; CONTROLLED STUDY; DNA TRANSFECTION; GENE AMPLIFICATION; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; RECOMBINANT GENE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0030597331     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00435-0     Document Type: Article

References (26)

1. Alt, F.W., Kellems, R.E., Bertino, J.R. and Schimke, R.T. (1978) Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. J. Biol. Chem. 253, 1357-1370.
2. Barsoum, J. (1990) Introduction of stable high-copy-number DNA into Chinese hamster ovary cells by electroporation. DNA Cell Biol. 9, 293-300.
3. Dente, L., Cesareni, G. and Cortese, R. (1983) pEMBL: a new family of single-stranded plasmids. Nucieic Acids Res. 11, 1645-1655.
4. Hemann, C., Gartner, E., Weidle, U.H., and Grummt, F. ( 1994) Highcopy expression vector based on amplification-promoting sequences. DNA Cell Biol. 13, 437-445.
5. Jeanpierre, M. (1987) A rapid method for the purification of DNA from blood. Nucleic Acids Res. 15, 9611.
6. Kaufman, R.J. and Sharp, P.A. (1982) Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J. Mol. Biol. 159, 601-621.
7. Kaufman, R.J., Wasley, L.C., Spiliotes, A.J., Gossels, S.D., Latt, S.A., Larsen, G.R. and Kay, R.M. (1985) Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells. Mol. Cell. Biol. 5, 1750-1759.
8. Kemball-Cook, G., Garner, I., Imanaka, Y., Nishimura, T., O'Brien, D.P., Tuddenham, E.G. and McVey, J.H. (1994) High-level production of human blood coagulation factors VII and XI using a new mammalian expression vector. Gene 139, 275-279.
9. Law, M.F., Lowy, D.R., Dvoretzky, I. and Howley, P.M. (1981) Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc. Natl. Acad. Sci. USA 78, 2727-2731.
10. McCormick, F., Trahey, M., Innis, M., Dieckmann, B. and Ringold, G. (1984) Inducible expression of amplified human beta interferon genes in CHO cells. Mol. Cell. Biol. 4, 166-172.
11. Milanesi, G., Barbanti-Brodano, G., Negrini, M., Lee, D., Corallini, A., Caputo, A., Grossi, M.P. and Ricciardi, R.P. (1984) BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli. Mol. Cell Biol. 4, 1551-1560.
12. Monaco, L., Tagliabue, R., Soria, M.R. and Uhlen, M. (1994) An in vitro amplification approach for the expression of recombinant proteins in mammalian cells. Biotechnol. Appl. Biochem. 20, 157-171.
13. Monaco, L., Marc, A., Eon-Duval, A., Acerbis, G., Distefano, G., Lamotte, D., Engasser, J.-M., Soria, M.R. and Jenkins, N. (1996) Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells and its effects on the sialylation of recombinant interferon-γ. Cytotechnology, in press.
14. Nagata, S., Tsuchiya, M., Asano, S., Yamamoto, O., Hirata, Y., Kubota, N., Oheda, M., Nomura, H. and Yamazaki, T, (1986) The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor. EMBO J. 5, 575-581.
15. Reed, K.C. and Mann, D.A. (1985) Rapid transfer of DNA from agarose gels to nylon memhranes. Nucleic Acids Res. 13, 7207-7221.
16. Reiser, W. and Hauser, H. (1987) Recombinant human interferon-ß from mammalian cell lines. Drug Res. 37, 482-485.
17. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York.
18. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
19. Subramani, S., Mulligan, R. and Berg, P. (1981) Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell Biol. 1, 854-864.
20. Sugden, B., Marsh, K. and Yates, J. (1985) A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus. Mol. Cell. Biol. 5, 410-413.
21. Urlaub, G. and Chasin, L.A. (1980) Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc. Natl. Acad. Sci. USA 77, 4216-4220.
22. Weidle, U.H., Buckel, P. and Grummt, F. (1988) A new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene. Gene 73, 427-437.
23. + transformants. Anal. Biochem. 203, 146-150.
24. Wirth, M., Bode, J., Zettlmeissl, G. and Hauser, H. (1988) Isolation of overproducing recombinant mammalian cell lines by a fast and simple selection procedure. Gene 73, 419-426.
25. Wurm, F.M., Gwinn, K.A. and Kingston, R.E. (1986) Inducible overproduction of the mouse c-myc protein in mammalian cells. Proc. Natl. Acad. Sci. USA 83, 5414-5418.
26. Zettlmeissl, G., Ragg, H. and Karges, H. (1987) Expression of biologically active human antithrombin III in Chinese hamster ovary cells. Bio/Technology 5, 720-725.