Allosteric regulation in a family of enterobacterial aspartate transcarbamylases: Intramolecular transmission of regulatory signals in chimeric enzymes

Journal of Molecular Biology

Volumn 262, Issue 2, 1996, Pages 258-269

  Cunin, Raymond ^a   Wales, Melinda E ^b   Van Vliet, Françoise ^a,c   De Staercke, Christine ^a,d   Scapozza, Leonardo ^b,e   Rani, Christiana Swarupa ^a   Wild, James R ^b  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b TEXAS A AND M UNIVERSITY   (United States)

^c Res Inst of the Ctr d'E   (Belgium)

^d GEORGIA STATE UNIVERSITY   (United States)

^e ETH ZURICH   (Switzerland)

Author keywords

Allostery; Enterobacterial aspartate transcarbamylases; Structure function of proteins

Indexed keywords

ASPARTATE CARBAMOYLTRANSFERASE;

ALLOSTERISM; AMINO ACID COMPOSITION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; ENTEROBACTERIACEAE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEUS VULGARIS; SERRATIA MARCESCENS; SIGNAL TRANSDUCTION;

ENTEROBACTERIACEAE; ESCHERICHIA COLI; PROTEUS VULGARIS; SERRATIA MARCESCENS;

EID: 0030595363     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0511     Document Type: Article

References (36)

1. Allewell, N. M. (1989). Escherichia coli aspartate transcarbamoylase structure, energetics, and catalytic and regulatory mechanisms. Annu. Rev. Biophys. Chem. 18, 71-92.
2. Beck, D. A., Kedzie, K. M. & Wild, J. R. (1989). Comparison of the aspartate transcarbamoylases from Serratia marcescens and Escherichia coli. J. Biol. Chem. 264, 16629-16637.
3. Cherfils, J., Vachette, P., Tauc, P. & Janin, J. (1987). The pAR5 mutation and the allosteric mechanism of Escherichia coli aspartate carbamoyltransferase. EMBO J. 6, 2843-2847.
4. De Staercke, C., Van Vliet, F., Xi, X. G., Swarupa Rani, C., Ladjimi, M., Jacobs, A., Triniolles, F., Hervé, G. & Cunin, R. (1995). Intramolecular transmission of the ATP regulatory signal in Escherichia coli aspartate transcarbamylase: specific involvement of a clustered set of amino acid interactions at an interface between regulatory and catalytic subunits. J. Mol. Biol. 246, 132-143.
5. m in enzyme reactions. Science, 116, 688.
6. England, P. & Hervé, G. (1992). Synergistic inhibition of Escherichia coli aspartate transcarbamylase by CTP and UTP: binding studies using continuous-flow dialysis. Biochemistry, 31, 9725-9732.
7. England, P. & Hervé, G. (1994). Involvement of the γ-phosphate of UTP in the synergistic inhibition of Escherichia coli aspartate transcarbamylase by CTP and UTP. Biochemistry, 33, 3913-3918.
8. Foltermann, K. F., Beck, D. A. & Wild, J. R. (1986). In vivo formation of hybrid aspartate transcarbamoylases from native subunits of divergent members of the family Enterobacteriaceae. J. Bacteriol. 167, 285-290.
9. Gerhart, J. C. & Holoubek, H. (1967). The purification of aspartate transcarbamylase of Escherichia coli and separation of its subunits. J. Biol. Chem. 242, 2886-2892.
10. Gouaux, J. E., Stevens, R. C. & Lipscomb, W. N. (1990). Crystal structures of aspartate carbamoyltransferase ligated with phosphonacetamide, malonate and CTP or ATP at 2.8 Å resolution and neutral pH. Biochemistry, 29, 7702-7715.
11. Hanes, C. S. (1932). Studies on plant amylases. I. The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley. Biochem. J. 26, 1406-1421.
12. Hervé, G. (1989). Aspartate transcarbamylase from Escherichia coli. In Allosteric Enzymes (Hervé, G., ed.), pp. 61-79, CRC Press, Boca Raton, FL.
13. Honzatko, R. B, Crawford, J. L., Monaco, H. L., Ladner, J. E., Edwards, B. F. P., Evans, D. R., Warren, S. G., Wiley, D. C., Ladner, R. C. & Lipscomb W. N. (1982). Crystal and molecular structures of native and CTP-liganded aspartate carbamoyltransferase from Escherichia coli. J. Mol. Biol. 160, 219-263.
14. Ke, H. M., Lipscomb, W. N., Cho, Y. & Honzatko, R. B. (1988). Complex of N-phosphonacetyl-L-aspartate with aspartate carbamoyltransferase. X-ray refinement, analysis of conformational changes and catalytic and allosteric mechanisms. J. Mol. Biol. 204, 725-747.
15. Kedzie, K. (1987). Characterization of the PyrB gene of Serratia marcescens and hybrid formation with the pyrB gene of Escherichia coli, leading to the production of chimeric ATCase. PhD dissertation, Texas A&M University, College Station, TX.
16. Kerbiriou, D., Hervé, G. & Griffin, J. H. (1977). An aspartate transcarbamylase lacking catalytic subunit interactions. J. Biol. Chem. 252, 2881-2890.
17. Krause, K. L., Volz, K. W. & Lipscomb, W. N. (1987). The 2.5 Å structure of aspartate carbamoyltransferase complexed with the bisubstrate analogue N-(phosphonacetyl)-L-aspartate. J. Mol. Biol. 193, 527-553.
18. Lipscomb, W. N. (1992). Activity and regulation in aspartate transcarbamylase. The Robert A. Welch Foundation Conference on Chemical Research XXXVI. Regulations of Proteins by Ligands. pp. 103-143, The Robert A. Welch Foundation, Houston, TX.
19. Lipscomb, W. N. (1994). Aspartate transcarbamylase from Escherichia coli: activity and regulation. Advances Enzymol. 68, 67-152.
20. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265-275.
21. Newton, C. J. & Kantrowitz, E. R. (1990). The regulatory subunit of Escherichia coli aspartate carbamoyltransferase may influence homotropic cooperativity and heterotropic interactions by a direct interaction with the loop containing residues 230-245 of the catalytic chain. Proc. Natl Acad. Sci. USA, 87, 2307-2313.
22. Nowlan, S. F. & Kantrowitz, E. R. (1985). Superproduction and rapid purification of Escherichia coli aspartate transcarbamylase and its catalytic subunit under extreme derepression of the pyrimidine pathway. J. Biol. Chem. 260, 14712-14716.
23. Pearlman, D. A., Case, D. A., Caldwell, J. C., Seibel, G. L., Singh, C., Weiner, P. and Kollman, P. (1992). AMBER 4.0, University of California, San Francisco, USA.
24. Prescott, L. M. & Jones, M. E. (1969). Modified method for the determination of carbamylaspartate. Anal. Biochem. 32, 408-419.
25. Ryde, U. (1995). Molecular dynamics simulations of alcohol dehydrogenase with a four-and five-coordinate catalytic zinc ion. Proteins: Struct. Funct. Genet. 21, 40-56.
26. Shanley, M. (1983). Properties of hybrid aspartate transcarbamylases. PhD dissertation, Texas A&M University, College Station, TX.
27. Shanley, M. S., Foltermann, K. F., O'Donovan, G. A. & Wild, J. R. (1984). Properties of hybrid aspartate transcarbamoylase formed with native subunits from divergent bacteria. J. Biol. Chem. 259, 12672-12677.
28. Stevens, R. C. & Lipscomb, W. N. (1992). A molecular mechanism for pyrimidine and purine control of aspartate transcarbamylase. Proc. Natl Acad. Sci. USA, 89, 5281-5285.
29. Stevens, R. C., Chook, Y. M., Cho, C. Y., Lipscomb, W. N. & Kantrowitz, E. R. (1991). Escherichia coli aspartate transcarbamylase: the probing of the crystal structure analysis via site-specific mutagenesis. Protein Engineering, 4, 391-408.
30. Wales, M. E., Strang, C. J., Swanson, R. & Wild, J. R. (1993). Modification of regulatory communication in aspartate transcarbamoylase. In Biocatalyst Design for Stability and Specificity (Himmel, M. E. & Georgiou, G., eds), American Chemical Society Symposium Series no. 516, pp. 197-209, American Chemical Society, Washington D.C.
31. Weiner, S. J., Kollman, P. A., Case, D. A., Singh, U. C., Ghio, C., Alagona, G., Profeta, S. & Weiner, P. A. (1984). A new force field for molecular mechanical simulation of nucleic acids and proteins. J. Am. Chem. Soc. 106, 765-784.
32. Wild, J. R. & Wales, M. E. (1990). Molecular evolution and genetic engineering of protein domains involving aspartate transcarbamoylase. Annu. Rev. Microbiol. 44, 193-218.
33. Wild, J. R., Foltermann, K. F. & O'Donovan, G. A. (1980). Regulatory divergence of aspartate transcarbamoylase within the Enterobacteriaceae. Arch. Biochem. Biophys. 201, 506-517.
34. Wild, J. R., Loughrey-Chen, S. J. & Corder, T. S. (1989). In the presence of CTP, UTP becomes an allosteric inhibitor of aspartate transcarbamylase. Proc. Natl Acad. Sci. USA, 86, 46-50.
35. Xi, X. G., Van Vliet, F., Ladjimi, M. M., De Wannemaeker, B., De Staercke, C., Piérard, A., Glansdorff, N., Hervé, G. & Cunin, R. (1990). Co-operative interactions between the catalytic sites in Escherichia coli aspartate transcarbamylase. Role of the C-terminal regions of the regulatory chains. J. Mol. Biol. 216, 375-384.
36. Xi, X. G., Van Vliet, F., Ladjimi, M. M., De Wannemaeker, B., De Staercke, C., Glansdorff, N., Piérard, A., Cunin, R. & Hervé, G. (1991). Heterotropic interactions in Escherichia coli aspartate transcarbamylase. Subunit interfaces involved in CTP inhibition and ATP activation. J. Mol. Biol. 220, 789-799.