Increased production of recombinant pyrroloquinoline quinone (PQQ) glucose dehydrogenase by metabolically engineered Escherichia coli strain capable of PQQ biosynthesis

Journal of Biotechnology

Volumn 49, Issue 1-3, 1996, Pages 239-243

  Sode, Koji ^a   Ito, Koji ^a   Witarto, Arief Budi ^a   Watanabe, Kazumoto ^a   Yoshida, Hiromi ^a   Postma, Pieter ^b  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Acinetobacter calcoaceticus; Glucose dehydrogenase; Metabolic engineering; Pyrroloquinoline quinone (PQQ)

Indexed keywords

BIOSYNTHESIS; COLIFORM BACTERIA; ENZYME KINETICS; GLUCOSE; METABOLISM;

ACINETOBACTER CALCOACETICUS; GLUCOSE DEHYDROGENASE; PYRROLOQUINOLINE QUINONE (PQQ);

GENETIC ENGINEERING;

APOENZYME; GLUCOSE DEHYDROGENASE; PYRROLOQUINOLINEQUINONE; RECOMBINANT PROTEIN;

ACINETOBACTER CALCOACETICUS; ARTICLE; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENETIC ENGINEERING; KLEBSIELLA PNEUMONIAE; NONHUMAN; PRIORITY JOURNAL;

ACINETOBACTER CALCOACETICUS; ALOCASIA MACRORRHIZOS; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE;

EID: 0030595043     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01540-4     Document Type: Article

References (17)

1. Ameyama, M., Nonobe, M., Shinagawa, E., Matsushita, K. and Adachi, O. (1985) Method of enzymatic determination of pyrroloquinoline quinone. Anal. Biochem. 151, 263-267.
2. Biville, F., Turlin, E. and Gasser, F. (1989) Cloning and genetic analysis of six pyrroloquinoline quinone biosynthesis genes in Methylobacterium organophilum DSM760. J. Gen. Microbiol. 135, 2917-2929.
3. Cleton-Jansen, A.M., Goosen, N., Fayet, O. and van de Putte, P. (1990) Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase. J. Bacteriol. 172, 6308-6315.
4. Goosen, N., Horsman, H.P.A., Huinen, R.G.M. and van de Putte, P. (1989) Acinetobacter calcoaceticus genes involved in biosynthesis of the coenzyme pyrrolo-quinoline-quinone: nucleotide sequence and expression in Escherichia coli K-12. J. Bacteriol. 171, 447-455.
5. Matsushita, K., Toyama, H., Ameyama, M., Adachi, O., Dewanti, A. and Duine, J.A. (1995) Soluble and membrane-bound quinoprotein D-glucose dehydrogenases of the Acinetobacter calcoaceticus: the binding process of PQQ to the apoenzymes. Biosci. Biotechnol. Biochem. 59, 1548-1555.
6. Meulenberg, J.J.M., Sellink, E., Loenen, W.A.M., Riegman, N.H., van Kleef, M. and Postma, P.W. (1990) Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli. FEMS Microbiol. Lett. 71, 337-344.
7. Meulenberg, J.J.M., Sellink, E., Riegman, N.H. and Postma, P.W. (1992) Nucleotide sequence and structure of the Klebsiella pneumoniae pqq operon. Mol. Gen. Genet. 232, 284-294.
8. Morris, C.J., Biville, F., Turlin, E., Lee, E., Ellermann, K., Fan, W., Ramamoorthi, R., Springer, A.L. and Lindstrom, M.E. (1994) Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequence of pqqD, pqqG, and pqqC. J. Bacteriol. 176, 1746-1755.
9. Sode, K., Nakasono, S., Tanaka, M. and Matsunaga, T. (1993) Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase. Biotechnol. Bioeng. 42, 251-254.
10. Sode, K. and Sano, H. (1994) Glu742 substitution to Lys enhances the EDTA tolerance of Escherichia coli PQQ glucose dehydrogenase. Biotechnol. Lett. 16, 455-460.
11. Sode, K., Witarto, A.B., Watanabe, K., Noda, K., Ito, S. and Tsugawa, W. (1994) Over-expression of PQQ glucose dehydrogenase in Escherichia coli under holo-enzyme forming condition. Biotechnol. Lett. 16, 1265-1268.
12. Sode, K., Yoshida, H., Matsumura, K., Kikuchi, T., Watanabe, M., Yasutake, N., Ito, S. and Sano, H. (1995) Elucidation of the region responsible for EDTA tolerance in PQQ glucose dehydrogenases by constructing Escherichia coli and Acinetobacter calcoaceticus chimeric enzymes. Biochem. Biophys. Res. Commun. 211, 268-273.
13. Turner, A.P.F., D'Costa, E.J. and Higgins, I.J. (1987) Enzymatic analysis using quinoprotein dehydrogenases. Ann. New York Acad. Sci. Enzyme Eng. 8 (501), 283-287.
14. van der Meer, R.A., Groen, B.W., van Kleef, M.A.G., Frank, J., Jongejan, J.A. and Duine, J.A. (1990) Isolation, preparation, and assay of pyrroloquinoline quinone. Methods Enzymol. 188, 260-283.
15. Velterop, J.S., Sellink, E., Meulenberg, J.J.M., David, S., Bulder, I. and Postma, P.W. (1995) Synthesis of pyrroloquinoline quinone in vivo and in vitro and detection of an intermediate in the biosynthetic pathway. J. Bacteriol. 177, 5088-5098.
16. Ye, L., Hammerle, M., Olsthoorn, A.J.J., Schuhmann, W., Schmidt, H.-L., Duine, J.A. and Heller, A. (1993) High current density 'Wired' quinoprotein glucose dehydrogenase electrode. Anal. Chem. 65, 238-241.
17. Yokoyama, K., Sode, K., Tamiya, E. and Karube, I. (1989) Integrated biosensor for glucose and galactose. Anal. Chim. Acta 218, 137-142.