Quantification of glioma cell invasion by confocal laser scanning microscopy in an in vitro co-culture system

Cancer Letters

Volumn 105, Issue 1, 1996, Pages 45-49

  Nygaard, Svein J T ^a   Tysnes, Ole Bjørn ^a  

^a UNIVERSITY OF BERGEN   (Norway)

Author keywords

Confocal laser scanning microscopy; Fluorescent dyes; Glioma cell invasion

Indexed keywords

FLUORESCENT DYE;

ARTICLE; BRAIN CELL; CANCER CELL CULTURE; CANCER INVASION; CELL AGGREGATION; CELL INVASION; CONTROLLED STUDY; GLIOMA CELL; LASER MICROSCOPY; MICROSCOPY; PRIORITY JOURNAL;

EID: 0030593491     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/0304-3835(96)04261-9     Document Type: Article

References (22)

1. [1] Kornblith, P.K., Welch, W.C. and Bradley, M.K. (1993) The future of therapy for glioblastomas. Surg. Neurol., 39, 538-543.
2. [2] Petersdorf, S.H. and Livingston, R.B. (1994) High dose chemotherapy for the treatment of malignant brain tumors. J. Neuro-Oncol., 20, 155-163.
3. [3] Bjerkvig, R., Laerum, O.D and Mella, O. (1986) Glioma cell interactions with fetal rat brain aggregates in vitro and with brain tissue in vivo. Cancer Res., 46, 4071-4079.
4. [4] Amar, A.P., DeArmond, S.J., Spencer, D., Stowring, L., Coopersmith, P., Ramos, D.M. and Rosenblum, M. (1994) Development of an in vitro extracellular matrix assay for studies of brain tumor cell invasion. J. Neuro-Oncol., 20, 1-15.
5. [5] DeArmond, S.J., Stowring, L., Amar, A., Coopersmith, P., Dougherty, D., Spencer, D., Mikkelsen, T and Rosenblum, M. (1994) Development of a non-selecting, non-perturbing method to study human brain tumor cell invasion in murine brain. J. Neuro-Oncol., 20, 27-34.
6. [6] Pedersen, P.-H., Marienhagen, K., Mørk, S. and Bjerkvig, R. (1993) Migratory pattern of fetal rat brain cells and human glioma cells in the adult rat brain. Cancer Res., 53, 5158-5165.
7. [7] Russel, D.S. and Rubinstein, L.J. (1989) Pathology of Tumours of the Nervous System, 5th. edn., pp. 421-448. Edward Arnold, London.
8. [8] Noel, A.C., Calle, A., Emonard, H.P., Nusgens, B.V., Simar, L., Foidart, J., Lapiere, C.M. and Foidart, J.M. (1991) Invasion of reconstituted basement membrane matrix is not correlated to the malignant metastatic cell phenotype. Cancer Res., 51, 405-414.
9. [9] Pedersen, P.-H., Rucklidge, G.J., Mørk, S.J., Terzis, A.J.A., Engebraaten, O., Lund-Johansen, M., Backlund, E.-O., Laerum, O.D. and Bjerkvig, R. (1994) Leptomeningeal tissue: a barrier against tumor cell invasion. J. Natl. Cancer Inst., 86, 1593-1599.
10. [10] Bjerkvig, R., Laerum, O.D. and Mella, O. (1986) Glioma cell interactions with fetal rat brain aggregates in vitro, and with brain tissue in vivo. Cancer Res., 46, 4071-4079.
11. [11] Engebraaten, O., Bjerkvig, R., Lund-Johansen, M., Wester, K., Pedersen, P.H., Mörk, S., Backlund, E.O. and Laerum, O.D. (1990) Interaction between human brain tumour biopsies and fetal rat brain tissue in vitro. Acta Neuropathol., 18, 130-140.
12. [12] Nygaard, S.J.T., Pedersen, P.H., Mikkelsen, T., Terzis, A.J.A., Tysnes, O.-B. and Bjerkvig, R. (1996) Glioma cell invasion visualized by scanning confocal laser microscopy in an in vitro co-culture system. Invasive Metast., in press.
13. [13] Akslen, L.A., Andersen, K.J. and Bjerkvig, R. (1988) Characteristics of human and rat glioma cells grown in a defined medium. Anticancer Res., 8, 797-804.
14. [14] Druckrey, H. (1971) Genotypes and phenotypes of ten inbred strains of BD-rats. Arzneim.-Forsch. (Drug Res.), 8, 1274-1278.
15. [15] Bjerkvig, R., Steinsvåg, S.K. and Laerum, O.D. (1986) Reaggregation of fetal rat brain cells in a stationary culture system I: Methodology and cell identification. In Vitro Cell Dev. B, 22, 180-192.
16. [16] Yuhas, J.M., Li, A.P., Martinez, A.D. and Ladman, A.J. (1977) A simplified method for production and growth of multicellular spheroids. Cancer Res., 37, 3639-3643.
17. [17] Sims, P.J., Waggoner, A.S., Wang, C.-H. and Hoffman, J.F. (1974) Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry, 13, 3315-3330.
18. [18] Honig, M.G. and Hume, R.I. (1986) Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J. Cell. Biol., 103, 171-187.
19. [19] Marienhagen, K., Pedersen, P.H., Terzis, A.J., Laerum, O.D., Arnold, H. and Bjerkvig, R. (1994) Interactions between fetal rat brain cells and mature brain tissue in vivo and in vitro. Neuropathol. Appl. Neurol., 20, 130-143.
20. [20] Engebraaten, O., Bjerkvig, R., Pedersen, P.-H. and Laerum, O.D. (1993) Effects of EGF, bFGF, NGF and PDGF(BB) on cell migratory and invasive capacities of human brain-tumour biopsies in vitro. Int. J. Cancer, 53, 209-214.
21. [21] Gundersen, H.J.G. and Jensen, E.B. (1987) The efficiency of systematic sampling insterology and its prediction. J. Microsc., 147, 229-263.
22. [22] Pedersen, P.-H., Ness, G.O., Engebråten, O., Bjerkvig, R., Lillehaug, J.R. and Laerum, O.D. (1994) Heterogenous response to the growth factors (EGF, PDGF(bb), TGF-a,b-bFGF, IL-2) on glioma spheroid growth, migration and invasion. Int. J. Cancer, 56, 255-261.