The effect of social experience on serotonergic modulation of the escape circuit of crayfish

Science

Volumn 271, Issue 5247, 1996, Pages 366-369

  Yeh, Shih Rung ^a   Fricke, Russell A ^b,c   Edwards, Donald H ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Office of the Commissioner   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ASTACOIDEA;

EID: 0030593037     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5247.366     Document Type: Article

References (26)

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4. E. F Coccaro, Int. Clin. Psychopharmacol. 7, 3 (1992), V. M. Linnoila and M. Virkkunen, J. Clin Psychiatry 53(suppl.), 46 (1992), S. Winberg, G. E. Nilsson, K. H Olsen, J. Comp. Physiol. A 170, 93 (1992); H. D Steklis, M J Raleigh, A Kling, K Tachiki, Am. J Primatol. 11, 133 (1986); O Cases et al , Science 268, 1763 (1995).
5. E. F Coccaro, Int. Clin. Psychopharmacol. 7, 3 (1992), V. M. Linnoila and M. Virkkunen, J. Clin Psychiatry 53(suppl.), 46 (1992), S. Winberg, G. E. Nilsson, K. H Olsen, J. Comp. Physiol. A 170, 93 (1992); H. D Steklis, M J Raleigh, A Kling, K Tachiki, Am. J Primatol. 11, 133 (1986); O Cases et al , Science 268, 1763 (1995).
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13. Juvenile (1 to 3 cm) crayfish (Procambarus clarkii) were hatched and raised in the laboratory, and adult (8 to 10 cm) crayfish were obtained commercially Both were isolated for longer than 1 month. Socially dominant and subordinate animals were created by pairing previously isolated crayfish for 12 days or longer Juvenile pairs were placed in mesh cages 5 cm in diameter in groups of eight in 20-gallon aquaria. Adult pairs were placed in individual small aquaria.
14. Crayfish of known dominance status were chilled to immobility, and the abdomen was removed and dissected dorsally to expose the ventral nerve cord The abdomen was pinned out in a petn dish and maintained in crayfish saline [A. Van Harreveld, Proc. Soc Exp. Biol. Med. 34, 428 (1936)] The LG axon was identified visually in the terminal connective and pen etrated in the proximal portion of the axon (Fig. 1). Shocks (0 3 ms) between 3 V and 7 5 V were applied to the ipsilateral third and fourth nerves of the terminal ganglion at 90-s intervals and evoked both sub-and superthreshold LG EPSPs. The stimulus series was repeated four times The preparation was then perfused with serotonin (100 μM in 7 preparations at the outset of these experiments, and 50 μM for the remaining 93 preparations) or with a 5-HT agonist (50 μM, 67 preparations), all at 1.2 ml/min After 45 min of perfusion, responses to four more stimulus series were recorded during continued perfusion. The effects on the LG response produced by the two concentrations of serotonin were indistinguishable. A saline wash followed; responses to a final set of stimuli were recorded after a 1-hour wash. Although the electrophysiology was performed with knowledge of the animals' dominance status, the effects of serotonin on the responses of all three types of animals were unmistakable and immediately distinguishable The responses of isolate and dominant animals were always greater than those of controls, whereas those of subordinates were always less.
15. S.-R. Yeh, R A Fricke, D. H. Edwards, data not shown
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18. 5-HT increased EPSPs in the reisolated subordinates (Fig. 4, filled inverted triangles in the left panel) 57.6 ± 16.5% (mean ± SEM) above those of controls, whereas EPSPs in the reisolated dominants (Fig. 4, right panel) were increased 42.1 ± 14 0% above those of controls, and EPSPs in isolates (Fig. 4, left columns of both panels) were 63 3 ± 8.8% above those of controls
19. 1 agonist reduced EPSPs in the reisolated subordinates (Fig. 4, open triangles in left panel) 3.7 ± 8.1% below those of controls, whereas EPSPs in the reisolated dominants (Fig. 4, open triangles in right panel) were increased 3.7 ± 7.4% above those of controls, and EPSPs in the isolates (Fig 4, open triangles in left columns of both panels) were increased 8 4 ± 10.3% above those of controls.
20. R. V Bovbjerg, Physiol Zool. 26, 173 (1953).
21. S. R. Yeh, R A. Fricke, D H. Edwards, unpublished results
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25. B. Zhang and R. Harris-Warnck, J. Exp. Biol. 190, 55 (1994).
26. We thank M. Homer, F. B. Krasne, and E. A Kravitz tor their thoughtful comments on this paper. Supported by NIH research grant RO1 NS26457 and NSF research grant IBN 94-23486.