Plant regeneration from adventitious roots of spinach (Spinacia oleracea L.) grown from protoplasts

Plant Science

Volumn 120, Issue 1, 1996, Pages 89-94

  Komai, Fuminori ^a,c   Masuda, Kiyoshi ^a   Harada, Takashi ^a   Okuse, Ichiro ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b HIROSAKI UNIVERSITY   (Japan)

^c Hokkaido Kitami Agric Exp Station   (Japan)

Author keywords

Plant regeneration; Protoplast; Somatic embryogenesis; Spinach (Spinacia oleracea); Tissue culture

Indexed keywords

ARTICLE; NONHUMAN; PLANT GROWTH; PLANT ROOT; PROTOPLAST; REGENERATION; SPINACH; TISSUE CULTURE;

SPINACIA OLERACEA;

EID: 0030592384     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(96)04463-9     Document Type: Article

References (24)

1. [1] E.E. Irish and T. Nelson, Sex determination in monoecious and dioecious plants. Plant Cell, 1 (1989) 737-744.
2. [2] B. Durand and R. Durand, Sex determination and reproductive organ differentiation in Mercurialis. Plant Sci., 80 (1991) 49-65.
3. [3] M. Bracale, E. Caporali, M.G. Galli, C. Longo, G. Marziani-Longo, G. Rossi, A. Spada, C. Soave, A. Falavigna, F. Raffaldi, E. Maestri, F.M. Restivo and F. Tassi, Sex determination and differentiation in Asparagus officinalis L. Plant Sci., 80 (1991) 67-77.
4. [4] S.L. Dellaporta and A. Calderon-Urrea, Sex determination in flowering plants. Plant Cell, 5 (1993) 1241-1251.
5. [5] C. Ainsworth, S. Crossley, V. Buchanan-Wollaston, M. Thangavelu and J. Parker, Male and female flowers of the dioecious plant sorrel show different patterns of MADS box gene expression. Plant Cell, 7 (1995) 1583-1598.
6. [6] S. Nohara, Genetic studies on Spinacia. Jpn. J. Bot., 1 (1923) 111-120.
7. [7] J.T. Rosa, Sex expression in spinach. Hilgardia, 1 (1925) 259-274.
8. [8] J. Janick, D.L. Mahoney and P.L. Pfahler, The trisomics of Spinacia oleracea. J. Hered., 50 (1959) 46-50.
9. [9] M.Kh. Chailakhyan and V.N. Khryanin, Effect of growth regulators and role of roots in sex expression in spinach. Planta, 142 (1978) 207-210.
10. [10] L. Culafic and M. Neskovic, Effect of growth substances on flowering and sex expression in isolated apical buds of Spinacia oleracea. Physiol. Plant., 48 (1980) 588-591.
11. [11] D. Ye, Y. Wu, P. Installe, M. Jacobs and I. Negrutiu, On the genetic control of the regeneration response in the dioecious Melandrium album. Sex. Plant Reprod., 5 (1992) 292-297.
12. [12] Y. Wu, P. Installe, S. Hinnisdaels, M. Jacobs and I. Negrutiu, Protoplast culture and female plant regeneration in the dioecious plant Melandrium album. Plant Cell Rep., 12 (1993) 211-215.
13. [13] M. Nishimura, R. Douce and T. Akazawa, A simple method for estimating intactness of spinach leaf protoplasts by glycolate oxidase assay. Plant Physiol., 78 (1985) 343-346.
14. [14] H. Nakagawa, H. Tanaka, T. Oba, T. Ogura and M. Iizuka, Callus formation from protoplasts of cultured Spinacia oleracea cells. Plant Cell Rep., 4 (1985) 148-150.
15. [15] F. Lefort and H. Greppin, Modifications of the protein profile in the protoplast of the apex of spinach (Spinacia oleracea L. cv. Nobel) during floral induction. Arch. Sci., 45 (1992) 69-84 (in French with English summary).
16. [16] T. Goto and M. Miyazaki, Plant regeneration from mesophyll protoplasts of Spinacia oleracea L. Plant Tissue Culture Lett., 9 (1992) 15-21.
17. [17] F. Komai, I. Okuse and T. Harada, Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L.). Plant Sci., 113 (1996) 203-208.
18. [18] T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15 (1962) 473-497.
19. [19] K. Masuda, A. Kudo-Shiratori and M. Inoue, Callus formation and plant regeneration from rice protoplasts purified by density gradient centrifugation. Plant. Sci., 62 (1989) 237-246.
20. [20] E.M. Frearson, J.B. Power and E.C. Cocking, The isolation, culture and regeneration of Petunia leaf protoplasts. Dev. Biol., 33 (1973) 130-137.
21. [21] J.M. Widholm, The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain. Tech., 47 (1972) 189-194.
22. [22] F. Komai, I. Okuse, K. Saga and T. Harada, Improvement on the efficiency of somatic embryogenesis from spinach root tissues by applying various sugars. J. Jpn. Soc. Hort. Sci., 65 (1996) 67-72.
23. [23] F. Komai, I. Okuse and T. Harada, Histological identification of somatic embryogenesis from excised root tissues of spinach (Spinacia oleracea L.). Plant Tissue Culture Lett., 12 (1995) 313-315.
24. [24] I. Okuse, K. Ono, K. Saga and F. Komai, In vitro culture of excised roots in spinach (Spinacia oleracea L.). Bull. Fac. Agric. Hirosaki Univ., 58 (1994) 46-55 (in Japanese with English summary).