Construction of new insecticidal Bacillus thuriagiensis recombinant strains by using the sporulation non-dependent expression system of cryIIIA and a site specific recombination vector

Journal of Biotechnology

Volumn 48, Issue 1-2, 1996, Pages 81-96

  Sanchis, Vincent ^a,b   Agaisse, Hervé ^a   Chaufaux, Josette ^b   Lereclus, Didier ^a,b  

^a INSTITUT PASTEUR   (France)

^b CEMAGREF   (France)

Author keywords

Bacillus thuringiensis; Biopesticides; Gene expression; Site specific recombination; Sporulation

Indexed keywords

BACTERIA; DNA; GENES; INSECTICIDES; PROTEINS;

BACILLUS THURINGIENSIS; BIOPESTICIDES; ENDOTOXIN; RECOMBINANT STRAINS; SITE SPECIFIC RECOMBINATION; SPORULATION;

GENETIC ENGINEERING;

ENDOTOXIN; INSECTICIDE;

ARTICLE; BACILLUS THURINGIENSIS; CONTROLLED STUDY; DNA RECOMBINATION; EXPRESSION VECTOR; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; SPOROGENESIS;

BACILLUS THURINGIENSIS; OSTRINIA; SPODOPTERA LITTORALIS;

EID: 0030592125     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01404-6     Document Type: Article

References (34)

1. Adams, L.F., Brown, K.L. and Whiteley, H.R. (1991) Molecular cloning and characterization of two genes encoding sigma factors that direct transcription from a Bacillus thuringiensis crystal protein gene promoter. J. Bacteriol. 173, 3846-3854.
2. Agaisse, H. and Lereclus, D. (1994a) Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant. J. Bacteriol. 176, 4734-4741.
3. Agaisse, H. and Lereclus, D. (1994b) Structural and functional analysis of the promoter region involved in full expression of the cryIIIA toxin gene of Bacillus thuringiensis. Mol. Microbiol. 13, 97-107.
4. Agaisse, H. and Lereclus, D. (1995) How does Bacillus thuringiensis produce so much insecticidal crystal protein. J. Bacteriol. 177, 6027-6032.
5. Angus, T.A. (1954) A bacterial toxin paralysing silkworm larvae. Nature 173, 54-56.
6. Arantes, O. and Lereclus, D. (1992) Construction of cloning vectors for Bacillus thuringiensis. Gene 108, 115-119.
7. Baum, J.A., Coyle, D.M., Gilbert, M.P., Jany, C.S. and Gawron-Burke, C. (1990) Novel cloning vectors for Bacillus thuringiensis. Appl. Environ. Microbiol. 56, 3420-3428.
8. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513-1523.
9. Bone, E.J. and Ellar, D.J. (1989) Transformation of Bacillus thuringiensis by electroporation. FEMS Microbiol. Lett. 58, 171-178.
10. Brown, K.L. and Whiteley, H.R. (1988) Isolation of a Bacillus thuringiensis RNA polymerase capable of transcribing crystal protein genes. Proc. Natl. Acad. Sci. USA 85, 4166-4170.
11. Brown, K.L. and Whiteley, H.R. (1990) Isolation of the second Bacillus thuringiensis RNA polymerase that transcribes from a crystal protein gene promoter. J. Bacteriol. 172. 6682-6688.
12. Chinery, S.A. and Hinchliffe, E. (1989) A novel class of vector for yeast transformation. Curr. Genet. 16, 21-25.
13. Dale. E. and Ow, D.W. (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88, 10558-10562.
14. De-Souza. M.T., Lecadet, M.-M. and Lereclus, D. (1993) Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription. J. Bacteriol. 175, 2952-2960.
15. Donovan, W.P., Dankocsik, C.C., Gilbert, M.P., Gawron-Burke, M.C., Groat, R.G. and Carlton, B.C. (1988) Amino acid sequence and entomocidal activity of the P2 crystal protein. An insect toxin from Bacillus thuringiensis var. kurstaki. J. Biol. Chem. 263, 561-567.
16. Gelernter, W.D. (1990) Targeting insecticide-resistant market: new development in microbial based products. In: Green, M.B., Moberg, W.K. and LeBaron, H. (Eds.), Targeting insecticide-resistant market: new development in microbial based products. American Chemical Society, Washington, DC, pp. 105-117.
17. González, J.M.J., Brown, B.J. and Carlton, B.C. (1982) Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxin among strains of B. thuringiensis and B. cereus. Proc. Natl. Acad. Sci. USA 79, 6951-6955.
18. Höfte, H. and Whiteley, H.R. (3989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242-255.
19. Horton, R.M., Hunt, H.D., Ho, S.N., Pullen, J.K. and Pease, L.R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61-68.
20. Kilby, N.J., Snaith, M.R. and Murray, J.A.H. (1993) Site-specific recombinases: tools for genome engineering. Trends Genet. 9, 413-421.
21. Lecadet, M.-M., Chaufaux, J., Ribier, J. and Lereclus, D. (1992) Construction of novel Bacillus thuringiensis strains with different insecticidal specificities by transduction and by transformation. Appl. Environ. Microbiol. 58, 840-849.
22. Lecadet, M.M., Blondel, M.O. and Ribier, J. (1980) Generalized transduction in Bacillus thuringiensis var. berliner 1715, using bacteriophage CP54 Ber. J. Gen. Microbiol. 121, 203-212.
23. Lereclus, D., Agaisse, H., Gominet, M. and Chaufaux, J. (1995) Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spo0A mutant. Bio/Technology 13, 67-71.
24. Lereclus, D., Delécluse, A. and Lecadet, M.-M. (1993) Diversity of Bacillus thuringiensis toxins and genes. In: Entwistle, P., Cory, J.S., Bailey, M.J. and Higgs, S. (Eds.), Diversity of Bacillus thuringiensis Toxins and Genes. Wiley, Chichester, UK, pp. 37-69.
25. Lereclus, D. and Arantes, O. (1992) spbA locus ensures the segregational stability of pHT1030, a novel type of Gram-positive replicon. Mol. Microbiol. 7, 35-46.
26. Lereclus, D., Vallade, M., Chaufaux, J., Arantes, O. and Rambaud, S. (1992) Expansion of insecticidal host range of Bacillus thuringiensis by in vivo genetic recombination. Bio/Technology 10, 418-421.
27. Lereclus, D., Arantes, O., Chaufaux, J. and Lecadet, M.-M. (1989) Transformation and expression of a cloned ∂-endotoxin gene in Bacillus thuringiensis. FEMS Microbiol. Lett. 60, 211-218.
28. Lereclus, D., Lecadet, M.-M., Ribier, J. and Dedonder, R. (1982) Molecular relationships among plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains. Mol. Gen. Genet. 186, 391-398.
29. Mahillon, J. and Lereclus, D. (1988) Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process. EMBO J. 7, 1515-1526.
30. Poitout, S. and Bues, R. (1970) Elevage de plusieurs espèces de Lépidoptères Noctuidae sur milieu artificiel riche et sur milieu artificiel simplifié. Ann. Zool. Ecol. Anim. 2, 79-91.
31. Palva, A., Vigren, G., Simonen, M., Rintala, H. and Laamanen, P. (1990) Nucleotide sequence of the Tetracycline resistance gene of pBC16 from Bacillus cereus. Nucleic Acids Res. 18, 1635.
32. Sanchis, V., Chaufaux, J. and Pauron, D. (1994) A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC ∂-endotoxin on Spodoptera littoralis and Bombyx mori. FEBS Lett. 353, 259-263.
33. Sanchis, V., Lereclus, D., Menou, G., Chaufaux, J., Guo, S. and Lecadet, M.-M. (1989) Nucleotide sequence and analysis of the N-terminal coding region of the Spodopteraactive δ-endotoxin gene of Bacillus thuringiensis aizawai 7.29. Mol. Microbiol. 3, 229-238.
34. Zuber, P., Healy, J.M. and Losick, R. (1987) Effects of plasmid propagation of a sporulation promoter on promoter utilization and sporulation in Bacillus subtilis. J. Bacteriol. 169, 461-469.