A novel Escherichia coli vector for oxygen-inducible high level expression of foreign genes

Gene

Volumn 176, Issue 1-2, 1996, Pages 269-272

  Gao, Bifeng ^a   Flores, Sonia C ^a   Bose, Swapan K ^a   McCord, Joe M ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Bacteria; Expression vector; Mn SOD; mut SOD; Mutated human; Oxidative stress; Oxygen; Paraquat; Plasmid; SOD; Superoxide dismutase

Indexed keywords

AMPICILLIN; BETA LACTAMASE; COMPLEMENTARY DNA; OXYGEN; PARAQUAT; RESTRICTION ENDONUCLEASE; SUPEROXIDE DISMUTASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PENICILLIN RESISTANCE; PRIORITY JOURNAL; PROMOTER REGION; TRANSCRIPTION TERMINATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030591806     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)00246-6     Document Type: Article

References (19)

1. Benov, L., Chang, L.Y., Day, B. and Fridovich, I. (1995) Copper, zinc superoxide dismutase in Escherichia coli: Periplasmic localization. Arch. Biochem. Biophys. 319, 508-511.
2. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L. and Boyer, H.W. (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95-113.
3. Carlioz, A. and Touati, D. (1986) Isolation of superoxide dismutase mutants in Escherichia coli: Is superoxide dismutase necessary for aerobic life? EMBO J. 5, 623-630.
4. Compan, I. and Touati, D. ( 1993) Interaction of six global transcription regulators in expression of manganese superoxide dismutase in Escherichia coli K-12. J. Bacteriol. 175, 1687-1696.
5. Gardner, P.R. and Fridovich, I. (1988) Transcription of the E. coli manganese-superoxide dismutase gene: redox effects in vitro. In: Cerutti, P.A., Fridovich, I. and McCord, J.M. (Eds.), Oxy-Radicals in Molecular Biology and Pathology. Alan R. Liss, New York, pp. 163-172.
6. Gardner, P.R. and Fridovich, I. (1993) NADPH inhibits transcription of the Escherichia coli manganese superoxide dismutase gene (sodA) in vitro. J. Biol. Chem. 268, 12958-12963.
7. Greenberg, J.T., Monach, P., Chou, J.H., Josephy, P.D. and Demple, B. (1990) Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. Proc. Natl. Acad. Sci. USA 87, 6181-6185.
8. Gregory, E.M. and Fridovich, I. (1973) Induction of superoxide dismutase by molecular oxygen. J. Bacteriol. 114, 543-548.
9. Hassan, H. and Fridovich, I. (1977) Regulation of the synthesis of superoxide dismutase in E. coli: induction by methyl viologen. J. Biol. Chem. 252, 7667-7672.
10. Hidalgo, E. and Demple, B. (1994) An iron sulfur center essential for transcriptional activation by the redox-sensing soxr protein. EMBO J. 13, 138-146.
11. Li, Z.Y. and Demple, B. (1994) SoxS, an activator of superoxide stress genes in Escherichia coli - Purification and interaction with DNA. J. Biol. Chem. 269, 18371-18377.
12. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275.
13. McCord, J.M. and Fridovich, I. (1969) Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049-6055.
14. McCord, J.M., Boyle, J.A., Day Jr., E.D., Rizzolo, L.J. and Salin, M.L. (1977) A manganese-containing superoxide dismutase from human liver. In: Michelson, A.M., McCord, J.M. and Fridovich, I. (Eds.), Superoxide and Superoxide Dismutase. Academic Press, London, pp. 129-138.
15. Nunoshiba, T., Hidalgo, E., Cuevas, C.F.A. and Demple, B. (1992) 2-stage control of an oxidative stress regulon - the escherichia-coli soxr protein triggers redox-inducible expression of the soxs regulatory gene. J. Bacteriol. 174, 6054-6060.
16. Privalle, C.T., Kong, S.E. and Fridovich, I. (1993) Induction of manganese-containing superoxide dismutase in anaerobic Escherichia coli by diamide and 1,10-phenanthroline - sites of transcriptional regulation. Proc. Natl. Acad. Sci. USA 90, 2310-2314.
17. Takeda, Y. and Avila, H. (1986) Structure and gene expression of the E. coli Mn-superoxide dismutase gene. Nucleic Acids Res. 14, 4577-4589.
18. Touati, D. (1983) Cloning and mapping of the manganese superoxide dismutase gene (sodA) of Escherichia coli K-12. J. Bacteriol. 155, 1078-1087.
19. Vieira, J. and Messing, J. (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259.