A multifunctional vector system for heterologous expression of proteins in Escherichia coli. Expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal of fusion peptide by Kex2 protease

Gene

Volumn 176, Issue 1-2, 1996, Pages 249-255

  Ghosh, Smita ^a   Lowenstein, John M ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

Affinity purification; Co expression vector; Expression in Escherichia coli; Hexahistidyl fusion protein; Hit 1 protein; Kex2 protease; Multifunctional expression vector; PLC( 1); Sequence specific proteolysis

Indexed keywords

HYBRID PROTEIN; NICKEL; PHOSPHOLIPASE C; PROTEINASE; ZINC;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHELATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 0030591758     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)00260-0     Document Type: Article

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