Construction of a dimeric form of glutamate dehydrogenase from Clostridium symbiosum by site-directed mutagenesis

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1297, Issue 2, 1996, Pages 149-158

  Pasquo, Alessandra ^a,b   Britton, K Linda ^b   Stillman, Timothy J ^b   Rice, Dave W ^b   Cölfen, Helmut ^c,e   Harding, Stephen E ^c   Scandurra, Roberto ^a   Engel, Paul C ^b,d  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^d UNIVERSITY COLLEGE DUBLIN   (Ireland)

^e Max Planck Institute for C   (Germany)

Author keywords

C. symbiosum; Glutamate dehydrogenase; Site directed mutagenesis; Subunit interaction

Indexed keywords

GLUTAMATE DEHYDROGENASE;

ARTICLE; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; CLOSTRIDIUM; ESCHERICHIA COLI; GEL FILTRATION; GENE EXPRESSION; IMMUNOBLOTTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET SPECTROPHOTOMETRY;

ASPARTIC ACID; BLOTTING, WESTERN; CIRCULAR DICHROISM; CLONING, MOLECULAR; CLOSTRIDIUM; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTAMATE DEHYDROGENASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; ULTRACENTRIFUGATION;

CLOSTRIDIUM; CLOSTRIDIUM SYMBIOSUM; ESCHERICHIA; ESCHERICHIA COLI; STAPHYLOCOCCUS PHAGE 187; TRIXIS;

EID: 0030591754     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(96)00017-9     Document Type: Article

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