Application of reversed-phase high-performance liquid chromatography in the purification of major allergens from grass pollen

Journal of Immunological Methods

Volumn 194, Issue 1, 1996, Pages 27-34

  Fahlbusch, Bärbel ^a   Muller W D ^a   Cromwell, O ^b   Jager L ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b ALLERGOPHARMA JOACHIM GANZER KG   (Germany)

Author keywords

Allergen purification; Chromatography; Grass extract; group 1 and group 5 allergens; reverse phase high performance liquid

Indexed keywords

ALLERGEN; PLANT EXTRACT;

AFFINITY CHROMATOGRAPHY; ALLERGENICITY; ALLERGY; ANTIGEN PURIFICATION; ARTICLE; GRASS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONTENT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

PHLEUM PRATENSE; POACEAE;

EID: 0030591594     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00008-7     Document Type: Article

References (31)

1. Breitenbach, M., Valenta, R., Breiteneder, H., Pettenburger, K., Scheiner, O., Rumpold, H. and Kraft, D. (1990) Introduction to cDNA cloning of plant allergens. In: A.H. Sehon, D. Kraft and G. Kunkel (Eds.), Epitopes of Atopic Allergens. UCB Institute of Allergy, Brussels, pp. 57-60.
2. Fahlbusch, B., Müller, W.D., Schlenvoigt, G., Jäger, L., Wahl, R. and Weber, B. (1993) Monoclonal antibody immunoassay for quantitative analysis of group V allergens in grass pollen extracts. Clin. Exp. Allergy 23, 747-754.
3. Fahlbusch, B., Schlenvoigt, G., Müller, W.D., Weber, B. and Jäger, L. (1994) A two-site monoclonal antibody ELISA for the quantification of group V allergens in grass extracts. Clin. Exp. Allergy 24, 752-757.
4. Griffith, I.J., Smith, P.M., Pollock, J., Theerakulipsut, P., Avjioglu, A., Davies, S., Hough, T., Singh, M.B., Simpson, R.J., Ward, L.D. and Knox, R.B. (1991) Cloning and sequencing of Lol p I, the major allergenic protein of rye-grass pollen. FEBS Lett. 279, 210-215.
5. Hewlett, B.J. and Clarke, A.E. (1981) Isolation and partial characterization of two antigenic glycoproteins from rye-grass (Lolium perenne) pollen. Biochem. J. 197, 695-706.
6. Johnson, P and Marsh, D.G. (1965) The isolation and characterization of allergens from the pollen of rye grass (Lolium perenne). Eur. Polym. J. 1, 63-77.
7. Jung, K., Fahlbusch, B., Müller, W.-D., Herrmann, D., Diener, C. and Jäger, L. (1989) Isolation of Timothy (Phleum pratense) allergens using affinity chromatography with monoclonal antibodies. Allerg. Immunol. 35, 287-294.
8. King, T.P., Hoffman, D., Lowenstein, H., Marsh, D.G., Platts-Mills, T.A.E. and Thomas, W. (1994) Allergen nomenclature. Bull. WHO 72, 797-806.
9. 2-terminal sequences of the group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerata. Clin. Exp. Allergy 22, 491-497.
10. Laffer, S., Vrtala, S., Duchene, M., Van Ree, R., Kraft, D., Scheiner, O. and Valenta, R. (1994) IgE-binding capacity of recombinant Timothy grass (Phleum pratense) pollen allergens. J. Allergy Clin. Immunol. 94: 88-94.
11. Marsh, D.G., Goodfriend, L., King, T.P., Lowenstein, H. and Platts-Mills, T.A.E. (1988) Allergen nomenclature. Allergy 43, 161-168.
12. Matthiesen, F. and Lowenstein, H. (1991) Group V allergens in grass pollens. I. Purification and characterization of the group V allergen from Phleum pratense pollen, Phl p V. Clin. Exp. Allergy 21, 297-307.
13. Mohapatra, S.S., Mohapatra, S., Yang, M., Ansari, A.A., Parronchi, P., Maggi, E. and Romagnani, S. (1994) Molecular basis of cross-reactivity among allergen-specific human T cells: T cell receptor V gene usage and epitope structure. Immunology 81, 15-20.
14. Mourad, W., Pelletier, G., Boulet, A., Islam, N., Valet, J.P. and Hebert, J. (1986) Allergenicity and cross-reactivity of rye grass pollen extracts revealed by monoclonal antibodies, J. Immunol. Methods 89, 53-59.
15. Müller, W.-D., Diener, C., Jung, K. and Jäger, L. (1988) Antigens of Timothy and other grass pollen extracts identified by monoclonal antibodies. Allergol. Immunopathol. 16, 315-320.
16. Müller, W.-D., Karamfilov, Th., Fahlbusch, B., Vogelsang, H. and Jäger, L. (1994) Analysis of human T cell clones reactive with group V grass pollen allergens. Int. Arch. Allergy Immunol. 105, 391-396.
17. Müller, W.-D., Karamfilov, T., Bufe, A., Fahlbusch, B., Wolf, I. and Jäger, L. (1996) Group 5 allergens of Timothy grass (Phl p 5) bear cross reacting T cell epitopes with group 1 allergens of rye grass (Lol p 1) Int. Arch. Allergy Immunol. 109, 352-355.
18. Obispo, T.M., Polo, F., Carreira, J. and Lombardero, M. (1994) A single purification method of group 1 allergens from Lolium perenne and Phleum pratense. Allergy Clin. Immunol. News 2 (suppl.), 307.
19. Perez, M., Ishioaka, G.Y., Walker, L.E. and Chesnut, R.W. (1990) cDNA cloning and immunological characterization of the rye grass allergen Lol p I. J. Biol. Chem. 265, 16210-16215.
20. Petersen, A., Becker, W.-M. and Schlaak, M. (1991) Epitope mapping of allergens and antigens of Timothy pollen extract. Appl. Theor. Electrophor. 2, 129-133.
21. Petersen, A., Becker, W.-M. and Schlaak, M. (1992) Characterization of isoforms of the major allergen Phl p V by two-dimensional immunoblotting and microsequencing. Int. Arch. Allergy Immunol. 98, 105-109.
22. Petersen, A., Becker, W.-M. and Schlaak, M. (1994) Epitope analysis of isoforms of the major allergen Phl p V by finger-printing and microsequencing. Clin. Exp. Allergy 24, 250-256.
23. Petersen, A., Becker, W.-M., Moll, H., Blumke, M. and Schlaak, M. (1995) Studies on the carbohydrate moieties of the Timothy grass pollen allergen Phl p I. Electrophoresis 116, 869-875.
24. Silvanovich, A., Astwood, J., Zhang, L., Olsen, E., Kisil, F., Sehon, A., Mohapatra, S. and Hill, R. (1991) Nucleotide sequence of three cDNAs coding for Poa p IX isoallergens from Kentucky blue grass pollen. J. Biol. Chem. 266, 1204-1210.
25. Singh, M.B. and Knox, R.B. (1985) Grass pollen allergens. Antigenic relationships detected using monoclonal antibodies and dot blotting immunoassay. Int. Arch. Allergy Appl. Immunol. 78, 300-304.
26. Singh, M.B., Smith, P.M. and Knox, B.R. (1990) Molecular biology of rye-grass pollen allergens. In: B.A. Baldo (Ed.), Molecular Approaches to the study of Allergens. Karger, Basel, pp. 100-120.
27. Singh, M.B., Hough, T., Theerakulipsut, P., Avjioglu, A., Davies, S., Smith, P.M., Taylor, P., Simpson, R.J., Ward, L.D., McCluskey, J., Puy, R. and Knox, B. (1991) Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: Intracellular targeting to the amyloplast. Proc. Natl. Acad. Sci. USA, 88, 1384-1388.
28. Thomas, W.R. and Chua, K.Y. (1993) Recombinant arthropod allergens. In Kraft D, Sehon AH(Eds). Molecular biology and immunology of allergens. CRC Press, Boca Raton, FL, pp. 21-29.
29. Van Ree, R., Driessen, M.N.B.M., Van Leeuwen, W.A., Stapel, S.D. and Aalberse, R.C. (1991) Variability of crossreactivity of IgE antibodies to group I and V allergens in eight grass pollen species. Clin. Exp. Allergy 22, 611-617.
30. Vieths, S., Schöning, B., Brockmann, S. and Aulepp, H. (1992) Untersuchungen zur Allergie gegen Lebensmittel pflanzlicher Herkunft: Herstellung und Charakterisierung von Obst-und Gemüseextrakten für serologische Untersuchungen. Dtsch. Lebensm.-Rundsch. 88, 239-243, 274-279.
31. Vrtala, S., Sperr W.R., Reimitzer, J., Van Ree, R., Laffer, S., Müller, W.D., Valent, P., Lechner, K., Rumpold, H., Kraft, D., Scheiner, O. and Valenta, R. (1993) cDNA cloning of a major allergen from Timothy grass (Phleum pratense) pollen - Characterization of the recombinant Phl p V allergen. J. Immunol. 151, 4773-4781.