Transition-metals facilitated electron transfer of semisynthetic myoglobin bearing bis(iminodiacetic acid) moiety

Tetrahedron Letters

Volumn 37, Issue 51, 1996, Pages 9233-9236

  Hamachi, Itaru ^a   Matsugi, Tomoaki ^a   Shinkai, Seiji ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; METAL ION; MYOGLOBIN;

ARTICLE; CHEMICAL REACTION; DRUG SYNTHESIS; ELECTRON TRANSPORT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ULTRAVIOLET SPECTROSCOPY;

EID: 0030590960     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(96)02192-2     Document Type: Article

References (12)

1. 1. Hilvert, D.; Kaiser, E. T. Biotech. Gen. Eng. Rev. 1987, 5, 297.
2. 2. Hamachi, I.; Tajiri, Y.; Shinkai, S. J. Am. Chem. Soc., 1994, 116, 7437.
3. 3. BOP: Benzotriazole-1-yl-oxy-tris(dimethylamino) phosphonium hexafluorophosphate DIEA: Diisopropyl ethylamine
4. 2O: C, 56.64, H, 5.46, N, 11.49%
5. 5. F. W. J. Teale, Biochim. Biophys. Acta, 1959, 35, 543.; T. Asakura, "Methods in Enzymology," ed. by S. Fliser and L. Packer, Academic Press, New York, 1978. Part C, p. 446; I. Hamachi, K. Nakamura, A. Fujita and T. Kunitake, J. Am. Chem. Soc., 1993, 115, 4966.
6. 5. F. W. J. Teale, Biochim. Biophys. Acta, 1959, 35, 543.; T. Asakura, "Methods in Enzymology," ed. by S. Fliser and L. Packer, Academic Press, New York, 1978. Part C, p. 446; I. Hamachi, K. Nakamura, A. Fujita and T. Kunitake, J. Am. Chem. Soc., 1993, 115, 4966.
7. 5. F. W. J. Teale, Biochim. Biophys. Acta, 1959, 35, 543.; T. Asakura, "Methods in Enzymology," ed. by S. Fliser and L. Packer, Academic Press, New York, 1978. Part C, p. 446; I. Hamachi, K. Nakamura, A. Fujita and T. Kunitake, J. Am. Chem. Soc., 1993, 115, 4966.
8. 2-Mb are 413, 542 and 579 nm. These values are identical to those for the corresponding forms of native Mb.
9. 2+).
10. 2+, whereas the α-helix region (two negative peaks at 220 and 208 nm and a positive peak at 190 nm) scarcely changes. Such a transition-metal effect was not observed for the reconstituted Mb with heme 5.
11. 9. K. Tsukahara, T. Okazawa, H. Takahashi, Y. Yamamoto, Inorg. Chem., 1986, 25, 4756 .
12. 10. S. V. Evans, G. D. Brayer, J. Mol. Biol., 1990, 213, 885.