Real-time fluorescence assay system for gene transcription: Simultaneous observation of protein/DNA binding, localized DNA melting, and mRNA production

Analytical Biochemistry

Volumn 243, Issue 2, 1996, Pages 234-244

  Dunkak, Kevin S ^a   Otto, Michael R ^a   Beechem, Joseph M ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUBBLE FORMATION; DNA; ESCHERICHIA COLI; FLUORESCENCE; MELTING; PROTEINS;

FLUORESCENCE ASSAY; GENE TRANSCRIPTIONS; MRNA TRANSCRIPTION; MULTICOLOR FLUORESCENCE; PHYSIOLOGICAL CONCENTRATIONS; SIMULTANEOUS DETERMINATIONS; SIMULTANEOUS OBSERVATION; SPECTROSCOPIC PROBES;

TRANSCRIPTION;

RNA POLYMERASE;

ANISOTROPY; ARTICLE; COMPLEX FORMATION; COVALENT BOND; DNA DENATURATION; FLUORESCENCE; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; RNA TRANSCRIPTION;

ESCHERICHIA COLI;

EID: 0030589613     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0511     Document Type: Article

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