Antigenicity of a viral peptide displayed on B-galactosidase fusion proteins is influenced by the presence of the homologous partner protein

FEMS Microbiology Letters

Volumn 145, Issue 1, 1996, Pages 77-82

  Corchero, J L ^a   Villaverde, A ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Foot and mouth disease virus; Fusion protein; Misfolding; Recombinant protein; Site A; VP1; Galactosidase

Indexed keywords

BETA GALACTOSIDASE; HYBRID PROTEIN; RECOMBINANT PROTEIN; VIRUS PROTEIN;

ANTIGENICITY; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOT AND MOUTH DISEASE VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STRUCTURE;

FOOT-AND-MOUTH DISEASE VIRUS;

EID: 0030589071     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00390-4     Document Type: Article

References (15)

1. [1] Giavedoni, L.D., Kaplan, G., Marcovecchio, F., Piccone, M.E. and Palma, E.L. (1991) Protection conferred by TrpE fusion proteins containing portions of the C-terminal region of capsid protein VP1 of foot-and-mouth disease virus. J. Gen. Virol. 72, 969-971.
2. [2] Bittle, J.L., Houghten, R.A., Alexander, H., Shinnick, T.M., Sutcliffe, J.G., Lerner, R.A., Rowlands, D.J. and Brown, F. (1982) Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature 298, 30-33.
3. [3] Lewis, S.A., Morgan, D.O. and Grubman, M.J. (1991) Expression, processing, and assembly of foot-and-mouth disease virus capsid structures in heterologous systems: induction of a neutralizing antibody response in guinea pigs. J. Virol. 65, 6572-6580.
4. [4] Lea, S., Hernández, J., Blakemore, W., Brocchi, E., Curry, S., Domingo, E., Fry, E., Abu-Ghazaleh, R., King, A., Newman, J., Stuart, D. and Mateu, M.G. (1994) The structure and antigenicity of a type C foot-and-mouth disease virus. Structure 2, 123-139.
5. [5] Mateu, M.G., Martinez, M.A., Capucci, L., Andreu, D., Giralt, E., Sobrino, F., Brocchi, E. and Domingo, E. (1990) A single amino acid substitution affects multiple overlapping epitopes in the major antigenic site of foot-and-mouth disease virus of serotype C. J. Gen. Virol. 71, 629-637.
6. [6] Benito, A., Mateu, M.G. and Villaverde, A. (1995) Improved mimicry of a foot-and-mouse disease virus antigenic site by a viral peptide displayed on β-galactosidase surface. Bio/Technology 13, 801-804.
7. [7] Studier, F.W. and Moffat, B.A. (1986) Use of the bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
8. [8] Schauder, B., Blöcker, H., Frank, R. and McCarthy, J.E.G. (1987) Inducible vectors incorporating the Escherichia coli atpE translational initiation region. Gene 52, 279-283.
9. [9] Vila, P., Corchero, J.L., Benito, A. and Villaverde, A. (1994) Ammonium-mediated reduction of plasmid copy number and recombinant gene expression in Escherichia coli. Biotechnol. Prog. 10, 648-651.
10. [10] Corchero, J.L., Viaplana, E., Benito, A. and Villaverde, A. (1996) The position of the heterologous domain can influence the solubility and proteolysis of β-galactosidase fusion proteins in E. coli. J. Biotechnol. (in press).
11. [11] Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
12. [12] Feliu, J.X. and Villaverde, A. (1994) An optimized ultrasonication protocol for bacterial cell disruption and recovery of β-galactosidase fusion proteins. Biotechnol. Tech. 8, 509-514
13. [13] Ullmann, A. (1992) One-step purification of hybrid proteins which have β-galactosidase activity. Gene 29, 27-31.
14. [14] Harrison, S.C. (1989) Finding the receptors. Nature 338, 205-206.
15. [15] Jacobson, R.H., Zhang, X-J., DuBose, R.F. and Matthews, B.W. (1994) Three-dimensional structure of β-galactosidase from E. coli. Nature 369, 761-766.