A monitoring system for energy transduction by bacteriorhodopsin liposomes

Journal of Biochemical and Biophysical Methods

Volumn 33, Issue 2, 1996, Pages 89-104

  Hendler, R W ^a   Mukhopadhyay, A K ^a   Smith, P D ^a   Cascio, H E ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Computer controlled system; Delta pH; Fluorescence probe; Light activation; Membrane potential; Photobleaching of probe; Proton pump; SCN selective electrode

Indexed keywords

BACTERIORHODOPSIN; LIPOSOME;

ARTICLE; AUTOMATION; COMPUTER PROGRAM; ELECTROCHEMISTRY; ENERGY TRANSFER; FLUORESCENCE; INFORMATION PROCESSING; ION SELECTIVE ELECTRODE; PRIORITY JOURNAL; TECHNIQUE;

BACTERIORHODOPSINS; CALIBRATION; DATA INTERPRETATION, STATISTICAL; ELECTROCHEMISTRY; ENERGY TRANSFER; HYDROGEN-ION CONCENTRATION; LIPOSOMES; MEMBRANE POTENTIALS; PROTONS; SOFTWARE;

EID: 0030589066     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(96)00017-6     Document Type: Article

References (12)

1. [1] Rigaud, J.-L., Paternostre, M.-T. and Bluzat, A., Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin. Biochemistry, 27 (1988) 2677-2688.
2. 1-ATPase from the thermophilic Bacillus PS3 co-reconstituted with bacteriorhodopsin into liposomes. J. Biol. Chem., 270 (1995) 21571-21578.
3. 1) reconstituted into liposomes and coreconstituted with bacteriorhodopsin. Eur. J. Biochem., 210 (1992) 287-291.
4. [4] Hendler, R.W., Setty, O.H., Shrager, R.I., Songco, D.C. and Friauf, W.S., Instrumentation and procedures for real time measurements of proton motive force, membrane potential, ΔpH, proton extrusion, and oxygen uptake in respiring cells and vesicles. Rev. Sci. Instr., 54 (1983) 1749-1755.
5. 2 concentration. Rev. Sci. Instr., 62, (1991) 1067-1071.
6. [6] Seigneuret, M., and Rigaud, J.-L., Use of the fluorescent probe pyranine to detect heterogeneous directions of proton movement in bacteriorhodopsin reconstituted large liposomes. FEBS Lett., 188 (1985) 101-106.
7. [7] Holloway, P.W., A simple procedure for removal of Triton X-100 from protein samples. Anal. Bioch., 53 (1973) 304-308.
8. [8] Smith, J.C., Russ, P., Cooperman, B.S. and Chance, B., Synthesis, structure determination, spectral properties, and energy-linked spectral responses of the extrinsic probe Oxonol V in membranes. Biochemistry, 15 (1976) 5094-5105.
9. [9] Cooper, C.E., Bruce, D. and Nicholls, P., Use of Oxonol V as a probe of membrane potential in proteoliposomes containing cytochrome oxidase in the submitochondrial orientation. Biochemistry, 29 (1990) 3859-3865.
10. [10] Smith, J.C., Potential-sensitive molecular probes in membranes of bioenergetic relevance. Biochim. Biophys. Acta, 1016 (1990) 1-28.
11. [11] Hellingwerf, K.J., Arents, J.C., Scholte, B.J. and Westerhoff, H.V., Bacteriorhodopsin in liposomes. II. Experimental evidence in support of a theoretical model. Biochim. Biophys. Acta, 547 (1979) 561-582.
12. [12] Rigaud, J.-L., Bluzat, A. and Buschlen, S., Incorporation of bacteriorhodopsin into large unilamellar liposomes by reverse phase evaporation. Biochem. Biophys. Res. Commun., 111 (1983) 373-382.