Expression, purification and crystallization of a cohesin domain from the cellulosome of Clostridium thermocellum

Journal of Biotechnology

Volumn 51, Issue 3, 1996, Pages 243-249

  Yaron, Sima ^a   Shimon, Linda J W ^b   Frolow, Felix ^b   Lamed, Raphael ^c   Morag, Ely ^b   Shoham, Yuval ^a   Bayer, Edward A ^b  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

cellulases; cellulosome; Clostridium thermocellum; cohesin domain; X ray crystallography

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BACTERIA; CELLULOSE; CRYSTALLIZATION; DNA; MOLECULAR STRUCTURE; PURIFICATION; X RAY CRYSTALLOGRAPHY;

CELLULOSOME; CLOSTRIDIUM THERMOCELLUM; COHESIN DOMAIN; DOCKERIN; PEPTIDE; SCAFFOLDIN;

ENZYMES;

CELLULASE; CELLULOSE; COHESIN; MULTIENZYME COMPLEX; UNCLASSIFIED DRUG;

CLOSTRIDIUM THERMOCELLUM; CONFERENCE PAPER; CONTROLLED STUDY; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOTECHNOLOGY; CELLULASE; CLONING, MOLECULAR; CLOSTRIDIUM; CRYSTALLIZATION; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI;

EID: 0030588690     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(96)01602-1     Document Type: Conference Paper

References (29)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1992) Short Protocols in Molecular Biology, 2nd edn. Wiley, New York.
2. Bayer, E.A., Setter, E. and Lamed, R. (1985) Organization and distribution of the cellulosome in Clostridium thermocellum. J. Bacteriol. 163, 552-559.
3. Bayer, E.A., Morag, E. and Lamed, R. (1994) The cellulosome - a treasure-trove for biotechnology. Trends Biotechnol. 12, 378-386.
4. Bayer, E.A., Morag, E., Shoham, Y., Tormo, J. and Lamed, R. (1996) The cellulosome: A cell-surface organelle for the adhesion to and degradation of cellulose. In: Fletcher, M. (Ed.), Bacterial adhesion: molecular and ecological diversity, Wiley-Liss, Inc., New York, pp. 155-182.
5. Béguin, P. and Aubert, J.-P. (1994) The biological degradation of cellulose. FEMS Microbiol. Lett. 13, 25-58.
6. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
7. Felix, C.R. and Ljungdahl, L.G. (1993) The cellulosome - the exocellular organelle of Clostridium. Annu. Rev. Microbiol. 47, 791-819.
8. Fierobe, H.-P., Bagnara-Tardif, C., Gaudin, C., Guerlesquin, F., Sauve, P., Belaich, A. and Belaich, J.-P. (1993) Purification and characterization of endoglucanase-C from Clostridium cellulolyticum - catalytic comparison with endoglucanase-A. Eur. J. Biochem. 217, 557-565.
9. L-protein reveals an unusual degree of internal homology. Mol. Microbiol. 8, 325-334.
10. Jancarik, J. and Kim, S.-H. (1991) Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Crystallogr. 24, 409-411.
11. Johnson, J.L. (1981) Genetic characterization. In: Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R. and Phillips, G.B. (Eds.), Manual Methods for General Bacteriology. American Society for Microbiology, Washington, DC, pp. 243-265.
12. Lamed, R. and Bayer, E.A. (1988a) The cellulosome of Clostridium thermocellum. Adv. Appl. Microbiol. 33, 1-46.
13. Lamed, R. and Bayer, E.A. (1988b) Cellulosomes from Clostridium thermocellum. Methods Enzymol. 160, 472-482.
14. Lamed, R. and Bayer, E.A. (1993) The cellulosome concept -a decade later! In: Shimada, K., Hoshino, S., Ohmiya, K., Sakka, K., Kobayashi, Y. and Karita, S. (Eds.), Genetics, Biochemistry and Ecology of Lignocellulose Degradation. Uni, Tokyo, pp. 1-12.
15. Lamed, R., Setter, E., Kenig, R. and Bayer, E.A. (1983) The cellulosome - a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities. Biotechnol. Bioeng. Symp. 13, 163-181.
16. Lamed, R., Morag (Morgenstern), E., Mor-Yosef, O. and Bayer, E.A. (1991) Cellulosome-like entities in Bacteroides cellulosolvens. Curr. Microbiol. 22, 27-33.
17. Lamed, R., Tormo, J., Chirino, A.J., Morag, E. and Bayer, E.A. (1994) Crystallization and preliminary X-ray analysis of the major cellulose-binding domain of the cellulosome from Clostridium thermocellum. J. Mol. Biol. 244, 236-237.
18. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208-218.
19. McPherson, A. (1982) Preparation and Analysis of Protein Crystals, Wiley, New York.
20. Morag, E., Halevy, I., Bayer, E.A. and Lamed, R. (1991) Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum. J. Bacteriol. 173, 4155-4162.
21. Morag, E., Lapidot, A., Govorko, D., Lamed, R., Wilchek, M., Bayer, E.A. and Shoham, Y. (1995) Expression, purification and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl. Environ. Microbiol. 61, 1980-1986.
22. Otwinowski, Z. (1993) Oscillation data reduction program. In: Sawyer, L., Isaacs, N. and Bailey, S. (Eds.), Proc. CCP4 Study Weekend: Data Collection and Processing, SERC Daresbury Laboratory, Warrington, UK. 29-30 Jan. 1993, pp. 56-62.
23. Poole, D.M., Morag, E., Lamed, R., Bayer, E.A., Hazlewood, G.P. and Gilbert, H.J. (1992) Identification of the cellulose binding domain of the cellulosome subunit S1 from Clostridium thermocellum. FEMS Microbiol. Lett. 99, 181-186.
24. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratories, Cold Spring Harbor, New York.
25. Shoseyov, O., Takagi, M., Goldstein, M.A. and Doi, R.H. (1992) Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. Proc. Natl. Acad. Sci. USA 89, 3483-3487.
26. Tokatlidis, K., Salamitou, S., Béguin, P., Dhurjati, P. and Aubert, J.-P. (1991) Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components. FEBS Lett. 291, 185-188.
27. Tormo, J., Lamed, R., Chirino, A.J., Morag, E., Bayer, E.A., Shoham, Y. and Steitz, T.A. (1996) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 15, 5739-5751.
28. Wu, J.H.D., Orme-Johnson, W.H. and Demain, A.L. (1988) Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose. Biochemistry 27, 1703-1709.
29. Yaron, S., Morag, E., Bayer, E.A., Lamed, R. and Shoham, Y. (1995) Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome. FEBS Lett. 360, 121-124.