Multiple Types of Chimeric Germ-Line Ig Heavy Chain Transcripts in Human B Cells Evidence for trans-Splicing of Human Ig RNA

Journal of Immunology

Volumn 157, Issue 8, 1996, Pages 3450-3459

  Fujieda, Shigeharu ^a,b   Lin, Ying Qiang ^a   Saxon, Andrew ^a   Zhang, Ke ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF FUKUI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN HEAVY CHAIN; INTERLEUKIN 4; MESSENGER RNA; PRIMER DNA;

ANTIBODY SPECIFICITY; ARTICLE; B LYMPHOCYTE; CELL LINE; GENE REARRANGEMENT; GENETICS; HUMAN; IMMUNOLOGY; IN VITRO STUDY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RNA SPLICING;

ANTIBODY DIVERSITY; B-LYMPHOCYTES; BASE SEQUENCE; CELL LINE; DNA PRIMERS; GENE REARRANGEMENT, B-LYMPHOCYTE; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; INTERLEUKIN-4; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RNA SPLICING; RNA, MESSENGER;

EID: 0030587822     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (64)

1. Esser, C., and A. Radbruch. 1990. Immunoglobulin class switching: molecular and cellular analysis. Annu. Rev. Immunol. 8:717.
2. Honjo, T., and T. Kataoka. 1978. Organization of immunoglobulin heavy chain genes and allelic deletion model. Proc. Natl. Acad. Sci. USA 75:2140.
3. Zhang, K., F. C. Mills, and A. Saxon. 1994. Switch circles from IL-4-directed ε class switching from human B lymphocytes: evidence for direct, sequential, and multiple step sequential switch from μ to ε Ig heavy chain gene. J. Immunol. 152:3427.
4. Fujieda, S., K. Zhang, and A. Saxon. 1995. IL-4 plus CD40 monoclonal antibody induces human B cells γ subclass specific isotype switching: switching to γ1, γ3, and γ4, but not γ2. J. Immunol. 155:2318.
5. Stavnezer-Nordgren, J., and S. Sirlin. 1986. Specificity of immunoglobulin heavy chain switch correlates with activity of germ-line heavy chain genes prior to switching. EMBO J. 5:95.
6. Lutzker, S., and F. W. Alt. 1988. Structure and expression of germ line immunoglobulin γ2b transcripts. Mol. Cell. Biol. 8:1849.
7. Radcliffe, G., Y. C. Lin, M. Julius, K. B. Marcu, and J. Stavnezer. 1990. Structure of germ line immunoglobulin α heavy-chain RNA and its location on polysomes. Mol. Cell. Biol. 10:382.
8. Rothman, P., Y. Y. Chen, S. Lutzker, S. C. Li, V. Stewart, R. Coffman, and F. W. Alt. 1990. Structure and expression of germ line immunoglobulin heavy-chain ε transcripts: interleukin-4 plus lipopolysaccharide-directed switching to Cε. Mol. Cell. Biol. 10:1672.
9. Lorenz, M., S. Jung, and A. Radbruch. 1995. Switch transcripts in immunoglobulin class switching. Science 267:1825.
10. Jung, S., K. Rajewsky, and A. Radbruch. 1993. Shutdown of class switch recombination by deletion of a switch region control element. Science 259:984.
11. Zhang, J., A. Bottaro, S. Li, V. Stewart, and F. W. Alt. 1993. A selective defect in IgG2b switching as a result of targeted mutation of the Iγ2b promoter and exon. EMBO J. 12:3529.
12. Xu, L., B. Gorham, S. C. Li, A. Bottaro, F. W. Alt, and P. Rothman. 1993. Replacement of germ-line ε promoter by gene targeting alters control of immunoglobulin heavy chain class switching. Proc. Natl. Acad. Sci. USA 90:3705.
13. Bottaro, A., R. Lansford, L. Xu, J. Zhang, P. Rothman, and F. W. Alt. 1994. S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process. EMBO J. 13:665.
14. Shimizu, A., M. C. Nussenzweig, T. R. Mizuta, P. Leder, and T. Honjo. 1989. Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse. Proc. Natl. Acad. Sci. USA 86:8020.
15. Shimizu, A., M. C. Nussenzweig, H. Han, M. Sanchez, and T. Honjo. 1991. trans-splicing as a possible molecular mechanism for the multiple isotype expression of the immunoglobulin gene. J. Exp. Med. 173:1385.
16. Han, H., M. Okamoto, T. Honjo, and A. Shimizu. 1991. Regulated expression of immunoglobulin trans-mRNA consisting of the variable region of a transgenic μ. chain and constant regions of endogenous isotypes. Int. Immunol. 12:1197.
17. Shimizu, A., and T. Honjo. 1993. Synthesis and regulation of trans-mRNA encoding the immunoglobulin ε heavy chain. FASEB J. 7:149.
18. Yaoita, Y., Y. Kumagai, K. Okumura, and T. Honjo. 1982. Expression of lymphocyte surface IgE does not require switch recombination. Nature 297:697.
19. Perlmutter, A. P., and W. Gilbert. 1984. Antibodies of the secondary response can be expressed without switch recombination in normal mouse B cells. Proc. Natl. Acad. Sci. USA 81:7189.
20. Chen, Y. W., C. Word, V. Dev, J. W. Uhr, E. S. Vitetta, and P. W. Tucker. 1986. Double isotype production by a neoplastic B cell line. J. Exp. Med. 164:562.
21. Kinashi, T., T. Godal, Y. Noma, N. R. Ling, Y. Yaoita, and T. Honjo. 1987. Human neoplastic B cells express more than two isotypes of immunoglobulins without deletion of heavy-chain constant-region genes. Genes Dev. 1:465.
22. Nolan-Willard, M., M. T. Berton, and P. Tucker. 1992. Coexpression of μ and γ1 heavy chains can occur by a discontinuous transcription mechanism from the same unrearranged chromosome. Proc. Natl. Acad. Sci. USA 89:1234.
23. Kunimoto, D. Y., M. C. Sneller, L. Claflin, J. F. Mushinski, and W. Strober. 1993. Molecular analysis of double isotype expression in IgA switching. J. Immunol. 150:1338.
24. Mackenzie, T., and H. M. Dosch. 1989. Clonal and molecular characteristics of the human IgE-committed B cell subset. J. Exp. Med. 169:407.
25. Chan, M. A., S. H. Benedict, H. M. Dosch, M. F. Hui, and L. D. Stein. 1990. Expression of IgE from a nonrearranged ε locus in cloned B lymphoblastoid cells that also express IgM. J. Immunol. 144:3563.
26. Fujieda, S., I. Noda, H. Saito, T. Hoshino, and M. Yagita. 1994. Swainsonine augments the cytotoxicity of human lymphokine-activated killer cells against autologous thyroid cancer cells. Arch. Otolaryngol. Head Neck Surg. 120:389.
27. Saxon, A., J. Feldhaus, and R. A. Robins. 1976. Single step separation of human T and B cells using AET treated SRBC rosettes. J. Immunol. Methods 12:285.
28. Zhang, K., E. A. Clark, and A. Saxon. 1991. CD40 stimulation provides an IFN-γ-independent and IL-4-dependent differentiation signal directly to human B cells for IgE production. J. Immunol. 146:1836.
29. Sherr, E., E. Macy, H. Kimata, M. Gilly, and A. Saxon. 1989. Binding the low affinity FcεR on B cells suppresses ongoing human IgE synthesis. J. Immunol. 142:481.
30. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156.
31. Gauchat, J. F., D. A. Lebman, R. L. Coffman, H. Gascan, and J. E. de Vries. 1990. Structure and expression of germ-line ε transcripts in human B cells induced by interleukin 4 to switch to IgE production. J. Exp. Med. 172:463.
32. Diaz-Sanchez, D., A. R. Dotson, H. Takenaka, and A. Saxon. 1994. Diesel exhaust particles induce local IgE production in vivo and alter the pattern of IgE messenger RNA isoform. J. Clin. Invest. 94:1417.
33. Neale, G. A. M., and G. R. Kitchingman. 1991. mRNA transcripts initiating within the human immunoglobulin mu heavy chain enhancer region contain a non-translatable exon and are extremely heterogenous at the 5′ end. Nucleic Acids Res. 19:2427.
34. Mills, F. C., J. S. Brooker, and R. D. Camerini-Otero. 1990. Sequences of human immunoglobulin switch regions: implications for recombination and transcription. Nucleic Acids Res. 18:7305.
35. Sideras, P., T. R. Mizuta, H. Kanamori, N. Suzuki, M. Okamoto, K. Kuze, H. Ohno, S. Doi, S. Fukuhara, M. S. Hassan, L. Hammarstöm, E. Smith, A. Shimizu, and T. Honjo. 1989. Production of sterile transcripts of Cγ genes in an IgM-producing human neoplastic B cell line that switches to IgG-producing cells. Int. Immunol 1:631.
36. Shapira, S. K., H. H. Jabara, C. P. Thienes, D. J. Ahern, D. Vercelli, H. J. Gould, and R. S. Geha. 1991. Deletional switch recombination occurs in interleukin-4-induced isotype switching to IgE expression by human B cells. Proc. Natl. Acad. Sci. USA 88:7528.
37. Ellison, J., and L. Hood. 1982. Linkage and sequence homology of two human immunoglobulin γ heavy chain constant region genes. Proc. Natl. Acad. Sci. USA 79:1984.
38. Max, E. E., J. Battey, R. Ney, I. R. Kirsch, and P. Leder. 1982. Duplication and deletion in the human immunoglobulin ε genes. Cell 29:691.
39. Zhang, K., A. Saxon, and E. E. Max. 1992. Two unusual forms of human immunoglobulin E encoded by alternative RNA splicing of ε heavy chain membrane exons. J. Exp. Med. 176:233.
40. Graff, C., and S. Gerondakis. 1990. RNA splicing generates alternate forms of germ-line immunoglobulin α heavy chain transcripts. Int. Immunol. 2:1143.
41. Li, S. C., P. B. Rothman, J. Zhang, C. Chan, D. Hirsh, and F. W. Alt. 1994. Expression of Iμ-Cγ hybrid germlime transcripts subsequent to immunoglobulin heavy chain class switching. Int. Immunol. 6:491.
42. Gerstein, R. M., W. N. Frankel, C. L. Hsieh, J. M. Durdik, S. Rath, J. M. Coffin, A. Nisonoff, and E. Selsing. 1990. Isotype switching of an immunoglobulin heavy chain transgene occurs by DNA recombination between different chromosomes. Cell 63:537.
43. Knight, K. L., M. Kingzette, M. A. Crane, and S. K. Zhai. 1995. Transchromosomally derived Ig heavy chains. J. Immunol. 155:684.
44. Murphy, W. J., K. P. Watkins, and N. Agabian. 1986. Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing. Cell 47:517.
45. Sutton, R. E., and J. C. Boothroyd. 1986. Evidence for trans splicing in trypanosomes. Cell 47:527.
46. Borst, P. 1986. Discontinuous transcription and antigenic variation in trypanosomes. Annu. Rev. Biochem. 55:701.
47. Nilsen, T. W. 1993. trans-splicing of nematode premessenger RNA. Annu. Rev. Microbiol. 47:413.
48. Solnick, D. 1985. Trans splicing of mRNA precursors. Cell 42:157.
49. Zaita, N., K. Torazawa, K. Shinozaki, and M. Sugiura. 1987. Trans splicing in vivo: joining of transcripts from the divided gene for ribosomal protein S12 in the chloroplasts of tobacco. FEBS Lett. 210:153.
50. Koller, B., H. Fromm, E. Galun, and M. Edelman. 1987. Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts. Cell 48:111.
51. Sen, D., and W. Gilbert. 1990. A sodium-potassium switch in the formation of four-stranded G4-DNA. Nature 344:410.
52. Kim, J., C. Cheong, and P. B. Moore. 1991. Tetramerization of an RNA oligonucleotide containing a GGGG sequence. Nature 351:331.
53. Cheong, C., and P. B. Moore. 1992. Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures. Biochemistry 31:8406.
54. Chiara, M. D., and R. Reed. 1995. A two-step mechanism for 5′ and 3′ splice-site pairing. Nature 375:510.
55. Sklenar, V., and J. Feigon. 1990. Formation of a stable triplex from a single DNA strand. Nature 345:836.
56. Kim, M. G., V. B. Zhurkin, R. L. Jernigan, and R. D. Camerini-Otero. 1995. Probing the structure of a putative intermediate in homologous recombination: the third strand in the parallel DNA triplex is in contact with the major groove of the duplex. J. Mol. Biol. 247:874.
57. Van Meervelt, L., D. Vlieghe, A. Dautant, B. Gallois, G. Precigoux, and O. Kennard. 1995. High-resolution structure of a DNA helix forming (C. G)*G base triplets. Nature 374:742.
58. Zhurkin, V. B., G. Raghunathan, N. B. Ulyanov, R. D. Camerini-Otero, and R. L. Jernigan. 1994. A parallel DNA triplex as a model for the intermediate in homologous recombination. J. Mol. Biol. 239:181.
59. Morgan, A. R. 1994. Three-standed (triplex) DNAs (RNAs): do they have a role in biology? Indian J. Biochem. Biophys. 31:83.
60. Miller, J. H., and H. M. Sobell. 1966. A molecular model for gene repression. Proc. Natl. Acad. Sci. USA 55:1201.
61. Morgan, A. R., and R. D. Wells. 1968. Specificity of the three-stranded complex formation between double-stranded DNA and single-stranded RNA containing repeating nucleotide sequences. J. Mol. Biol. 37:63.
62. Murray, N. L., and A. R. Morgan. 1973. Enzymatic and physical studies on the triplex dTn:dAn:rUn. Can. J. Biochem. 51:436.
63. Ravetch, J. V., U. Siebenlist, S. Korsmeyer, T. Waldmann, and P. Leder. 1981. Structure of the human immunoglobulin μ locus: characterization of embryonic and rearranged J and D genes. Cell 27:583.
64. Kitani, A., and W. Strober. 1993. Regulation of Cγ subclass germ-line transcripts in human peripheral blood B cells. J. Immunol. 151:3478.