Characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in Entamoeba histolytica

Molecular and Biochemical Parasitology

Volumn 82, Issue 1, 1996, Pages 81-90

  Wöstmann, Claudia ^a   Liakopoulos, Dimitris ^b   Ciechanover, Aaron ^c   Bakker Grunwald, Tilly ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Entamoeba histolytica; Heat shock; Ubiquitin

Indexed keywords

MESSENGER RNA; UBIQUITIN; UBIQUITIN CONJUGATING ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENTAMOEBA HISTOLYTICA; GENE EXPRESSION REGULATION; HEAT SHOCK; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALIA; BOVINAE; ENTAMOEBA HISTOLYTICA;

EID: 0030581661     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(96)02725-9     Document Type: Article

References (32)

1. 1 Note: Nucleotide sequence data reported in this paper are available in the EMBL data bank under the accession number X98567.
2. [1] Ciechanover, A. (1994) The ubiquitin-proteasome proteolytic pathway. Cell 79, 13-21.
3. [2] Finley, D. and Chau, V. (1991) Ubiquitination. Annu. Rev. Cell Biol. 7, 25-69.
4. [3] Jentsch, S. (1992) The ubiquitin-conjugation system. Annu. Rev. Genet. 26, 179-207.
5. [4] Krebber, H., Wöstmann, C. and Bakker-Grunwald, T. (1994) Evidence for the existence of a single ubiquitin gene in Giardia lamblia. FEBS Lett. 343, 234-236.
6. [5] Finley, D., Özkaynak, E. and Varshavsky, A. (1987) The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation and other stresses. Cell 48, 1035-1046.
7. [6] Müller-Taubenberg, A., Westphal, M., Jaeger, E., Noegel, A. and Gerisch, G. (1988) Complete cDNA sequence of a Dictyostelium discoideum ubiquitin with a carboxy-terminal tail and identification of the protein using an anti-peptide antibody. FEBS Lett. 229, 273-278.
8. [7] Swindle, J., Ajioka, J., Eisen, H., Sanwal, B., Jacquemot, C., Browder, Z. and Buck, G. (1988) The genomic organization and transcription of the ubiquitin genes of Trypanosoma cruzi. EMBO J. 7, 1121-1127.
9. [8] Wöstmann, C., Tannich, E. and Bakker-Grunwald, T. (1992) Ubiquitin of Entamoeba histolytica deviates in six amino acid residues from the consensus of all other known ubiquitins. FEBS Lett. 308, 54-58.
10. [9] Diamond, L.S., Harlow, D.R. and Cunnick, C.C. (1978) A new medium for axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg. 72, 431-432.
11. [10] Tannich, E., Bruchhaus, I., Walter, R.D. and Horstmann, R.D. (1991) Pathogenic and non-pathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase. Mol. Biochem. Parasitol. 49, 61-72.
12. [11] Chirgwin, J.M., Przybyla, A.E., MacDonald, R.J. and Rutter, W.J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
13. [12] Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Erlich, H.A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
14. [13] Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. [14] Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
16. [15] Tannich, E., Nickel, R., Buss, H. and Horstmann, R.D. (1992) Mapping and partial sequencing of the genes coding for two different cysteine proteinases in pathogenic Entamoeba histolytica. Mol. Biochem. Parasitol. 54, 109-111.
17. [16] Gietz, R.D. and Sugino, A. (1988) New yeast-Escherichia coli shuttle vector constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74, 527-534.
18. [17] Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D. and Smith, J.A. (1987) Current protocols in molecular biology. John Wiley and Sons, New York.
19. [18] Scholze, H. and Tannich, E. (1994) Cysteine endopeptidases of Entamoeba histolytica. Methods Enzymol. 244, 512-523.
20. [19] Hershko, A., Heller, H., Elias, S. and Ciechanover, A. (1983) Compontents of ubiquitin-protein ligase system. J. Biol. Chem. 258, 8206-8214.
21. [20] Bruchhaus, I., Leippe, M., Lioutas, C. and Tannich, E. (1993) Unusual gene organization in the protozoan parasite Entamoeba histolytica. DNA Cell Biol. 12, 925-933.
22. [21] Jentsch, S., Seufert, W. and Hauser, H.P. (1991) Genetic analysis of the ubiquitin system. Biochim. Biophys. Acta 1089, 127-139.
23. [22] Keeling, P.J. and Doolittle, W.F. (1995) Concerted evolution in protists: recent homogenization of a polyubiquitin gene in Trichomonas vaginalis. J. Mol. Evol. 41, 556-562.
24. [23] Hershko, A. and Ciechanover, A. (1992) The ubiquitin system for protein degradation. Annu. Rev. Biochem. 61761-807.
25. [24] Lowrie, D.J., Giffin, B.F. and Ventullo, R.M. (1993) The ubiquitin-ligase system in Trypanosoma brucei. Am. J. Trop. Med. Hyg. 49, 545-551.
26. [25] Sogin, M.L., Gunderson, J.H., Elwood, H.J., Alonso, R.A. and Peattie, D.A. (1989) Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia. Science 243, 75-77.
27. [26] Kabnick, K.S. and Peattie, D.A. (1991) Giardia - a missing link between prokaryotes and eukaryotes. Am. Sci. 79, 34-43.
28. [27] Wenzel, T. and Baumeister, W. (1993) Thermoplasma acidophilum proteasomes degrade partially unfolded and ubiquitin-associated proteins. FEBS Lett. 326, 215-218.
29. [28] Scholze, H., Frey, S., Cejka, Z. and Bakker-Grunwald, T. (1996) Evidence for the existence of both proteasomes and a novel high-molecular weight peptidase in Entamoeba histolytica. J. Biol. Chem. 271, 6212-6216.
30. [29] Manning-Cela, R., Meraz, M.A., Hernandez, J.M. and Meza, I. (1994) Actin mRNA levels and actin synthesis during the encystation of Entamoeba invadens. J. Eukaryot. Microbiol. 41, 360-365.
31. [30] Calvo-Mendez, C., Villagomez-Castro, J.C. and Lopez-Romero, E. (1993) Rapid loss of ornithine decarboxylase activity during encystation of Entamoeba invadens. FEMS Microbiol. Lett. 112, 131-134.
32. [31] Hayashi, S., Murakami, Y. and Matsufuji, S. (1996) Ornithine decarboxylase antizyme: a novel type of regulatory protein. Trends Biochem. Sci. 21, 27-30.