Two versatile eukaryotic expression vectors permitting epitope tagging, radiolabelling and nuclear localisation of expressed proteins

Gene

Volumn 168, Issue 2, 1996, Pages 165-167

  Georgiev, Oleg ^a   Bourquin, Jean Pierre ^a   Gstaiger, Matthias ^a   Knoepfel, Lea ^a   Schaffner, Walter ^a   Hovens, Christopher ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

FLAG tag; Heart muscle kinase; In vitro transcription; In vitro translation

Indexed keywords

EPITOPE; PHOSPHOTRANSFERASE; PROTEIN; COMPLEMENTARY DNA; PROTEIN KINASE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; EUKARYOTE; EXPRESSION VECTOR; GENE EXPRESSION; GENE LOCATION; ISOTOPE LABELING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CELL NUCLEUS; COMPARATIVE STUDY; ENZYMOLOGY; EUKARYOTIC CELL; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HEART MUSCLE; MAMMAL; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PLASMID; PROTEIN SYNTHESIS; RESTRICTION MAPPING; SEQUENCE TAGGED SITE; SIMIAN VIRUS 40; TRANSPOSON;

ANIMALIA; EUKARYOTA; MAMMALIA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL NUCLEUS; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; DNA, COMPLEMENTARY; EPITOPES; EUKARYOTIC CELLS; GENETIC VECTORS; MAMMALS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; MYOCARDIUM; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN KINASES; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEQUENCE TAGGED SITES; SIMIAN VIRUS 40; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 0030580709     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(95)00764-4     Document Type: Article

References (9)

1. Blanar, M.A. and Rutter, W.J.: Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos. Science 256 (1992) 1014-1018.
2. Gstaiger, M., Knopfel, L., Georgiev, O., Schaffner, W. and Hovens, M.C.: Bob1, a B-cell coactivator of octamer binding transcription factors. Nature 373 (1995) 360-362.
3. Höller, M., Westin, G., Jiricny, J. and Schaffner, W.: Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes Dev. 2 (1988) 1127-1135.
4. Hopp, H.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlon, P.J.: A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6 (1988) 1204.
5. Huang, B., Shi, Z. and Tsai, M.D.: A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151 (1994) 143-145.
6. Fields, S. and Song, O.: A novel genetic system to detect protein-protein interactions. Nature 340 (1989) 245-246.
7. Kemler, I., Bucher, E., Seipel, K., Müller-Immerglück, M.M. and Schaffner, W.: Promoters with the octamer DNA motif (ATGCAAAT) can be ubiquitous or cell type-specific depending on binding affinity of the octamer site and Oct-factor concentration. Nucleic Acids Res. 19 (1991) 237-242.
8. Pallisgaard, N., Pedersen, F.S., Birkelund, S. and Jørgensen, P.: A common multiple cloning site in a set of vectors for expression of eukaryotic genes in mammalian, insect and bacterial cells. Gene 138 (1994) 115-118.
9. Schreiber, E., Tobler, A., Malipiero, U., Schaffner, W. and Fontana, A.: cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif. Nucleic Acids Res. 21 (1993) 253-258.