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Volumn 37, Issue 46, 1996, Pages 8431-8434

Boc SPPS of two hydrophobic peptides using a 'solubilising tail' strategy: Dodecaalanine and chemotactic protein 1042-55

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOTACTIC PEPTIDE;

EID: 0030580346     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(96)01910-7     Document Type: Article
Times cited : (23)

References (15)
  • 5
    • 85136584184 scopus 로고    scopus 로고
    • note
    • 4Gly-PAM-resin on a 1 mmole scale. Boc-L-amino acids (2eq) were activated with HBTU and coupled for 10 minutes (average coupling by ninhydrin assay: 99.8%, all couplings performed once) using the in-situ neutralisation method described in Reference 3.
  • 6
    • 0011876324 scopus 로고    scopus 로고
    • note
    • 4-Gly-OH, cleaved at an intermediate stage of the synthesis of dodecaalanine, showed that no peptides attributable to incomplete displacement of bromine by Boc-Ala cesium salt were present.
  • 9
    • 85136577389 scopus 로고    scopus 로고
    • note
    • 4-Gly-PAM-resin for 10 minutes using the in-situ neutralisation method described in Reference 3. All couplings were performed once. The average stepwise Ala coupling (ninhydrin assay) was 99.7%.
  • 10
    • 85136590937 scopus 로고    scopus 로고
    • note
    • 4-Gly-PAM-resin was cleaved with HF:p-cresol (9:1 v:v) at 0°C for one hour. HF was removed in-vacuo and the peptide construct was precipitated with ether. The water-insoluble precipitate was dissolved in 3 ml TFA, diluted with water, and lyophilised. The peptide-construct dissolved readily in water after lyophilisation.
  • 11
    • 0011873470 scopus 로고    scopus 로고
    • note
    • 9. HPLC analyses: Vydac (Hesperia, CA) C4 4.6 x 150 mm column, 5-85%B over 40 minutes at 1 ml/min. Solvent A: 0.1% TFA; Solvent B: 0.1% TFA, 10% water, 90% acetonitrile.
  • 12
    • 0011873431 scopus 로고    scopus 로고
    • note
    • 10. Preparative HPLC: Vydac C4 25 x 250 mm column, 0-60%B over 60 minutes at 8 ml/min (solvents as above).
  • 13
    • 0011833474 scopus 로고    scopus 로고
    • note
    • 4-G-OH (2.0 mg in 900 μl water, a clear solution) were treated with 100 μl of triethylamine for 10 minutes (pH 12.2). The resultant peptide precipitates were collected by centrifugation. After washing six times with 1 ml water the precipitates were shaken with a mixture of 20 μl TFA and 980 μl acetic acid. The suspensions of peptides which resulted were filtered for analysis by ES-MS.
  • 14
    • 85136590001 scopus 로고    scopus 로고
    • note
    • 8 97.54%.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.