-
1
-
-
0026470175
-
-
1. a) Bedford, J., Hyde, C., Johnson, T., Jun, W., Owen, D., Quibell, M., Sheppard, R.C. Int. J. Peptide Protein Res. 1992, 40, 300-307,
-
(1992)
Int. J. Peptide Protein Res.
, vol.40
, pp. 300-307
-
-
Bedford, J.1
Hyde, C.2
Johnson, T.3
Jun, W.4
Owen, D.5
Quibell, M.6
Sheppard, R.C.7
-
3
-
-
0027299860
-
-
2. Lackmann, M., Rajasekariah, P., Iismaa, S.E., Jones, G., Cornish, C.J., Hu, S., Simpson, R.J., Moritz, R.L., Geczy, C.L. J. Immunol. 1993, 150, 2981-2991.
-
(1993)
J. Immunol.
, vol.150
, pp. 2981-2991
-
-
Lackmann, M.1
Rajasekariah, P.2
Iismaa, S.E.3
Jones, G.4
Cornish, C.J.5
Hu, S.6
Simpson, R.J.7
Moritz, R.L.8
Geczy, C.L.9
-
4
-
-
0026486811
-
-
3. Schnölzer, M., Alewood, P., Alewood, D., Kent, S.B.H. Int. J. Peptide Protein Res. 1992, 40, 180-193.
-
(1992)
Int. J. Peptide Protein Res.
, vol.40
, pp. 180-193
-
-
Schnölzer, M.1
Alewood, P.2
Alewood, D.3
Kent, S.B.H.4
-
5
-
-
85136584184
-
-
note
-
4Gly-PAM-resin on a 1 mmole scale. Boc-L-amino acids (2eq) were activated with HBTU and coupled for 10 minutes (average coupling by ninhydrin assay: 99.8%, all couplings performed once) using the in-situ neutralisation method described in Reference 3.
-
-
-
-
6
-
-
0011876324
-
-
note
-
4-Gly-OH, cleaved at an intermediate stage of the synthesis of dodecaalanine, showed that no peptides attributable to incomplete displacement of bromine by Boc-Ala cesium salt were present.
-
-
-
-
7
-
-
85004802188
-
-
6. a) Baleux, F., Calas, B., Mery, J. Int. J. Peptide Protein Res. 1986, 28, 22-28;
-
(1986)
Int. J. Peptide Protein Res.
, vol.28
, pp. 22-28
-
-
Baleux, F.1
Calas, B.2
Mery, J.3
-
8
-
-
0000930025
-
-
b) Baleux, F., Daunis, J., Jacquier, R. Tetrahedron Letts. 1984, 25, 5893-5896.
-
(1984)
Tetrahedron Letts.
, vol.25
, pp. 5893-5896
-
-
Baleux, F.1
Daunis, J.2
Jacquier, R.3
-
9
-
-
85136577389
-
-
note
-
4-Gly-PAM-resin for 10 minutes using the in-situ neutralisation method described in Reference 3. All couplings were performed once. The average stepwise Ala coupling (ninhydrin assay) was 99.7%.
-
-
-
-
10
-
-
85136590937
-
-
note
-
4-Gly-PAM-resin was cleaved with HF:p-cresol (9:1 v:v) at 0°C for one hour. HF was removed in-vacuo and the peptide construct was precipitated with ether. The water-insoluble precipitate was dissolved in 3 ml TFA, diluted with water, and lyophilised. The peptide-construct dissolved readily in water after lyophilisation.
-
-
-
-
11
-
-
0011873470
-
-
note
-
9. HPLC analyses: Vydac (Hesperia, CA) C4 4.6 x 150 mm column, 5-85%B over 40 minutes at 1 ml/min. Solvent A: 0.1% TFA; Solvent B: 0.1% TFA, 10% water, 90% acetonitrile.
-
-
-
-
12
-
-
0011873431
-
-
note
-
10. Preparative HPLC: Vydac C4 25 x 250 mm column, 0-60%B over 60 minutes at 8 ml/min (solvents as above).
-
-
-
-
13
-
-
0011833474
-
-
note
-
4-G-OH (2.0 mg in 900 μl water, a clear solution) were treated with 100 μl of triethylamine for 10 minutes (pH 12.2). The resultant peptide precipitates were collected by centrifugation. After washing six times with 1 ml water the precipitates were shaken with a mixture of 20 μl TFA and 980 μl acetic acid. The suspensions of peptides which resulted were filtered for analysis by ES-MS.
-
-
-
-
14
-
-
85136590001
-
-
note
-
8 97.54%.
-
-
-
|