Laser manipulation and UV induced single molecule reactions of individual DNA molecules

Journal of Biotechnology

Volumn 52, Issue 2, 1996, Pages 65-73

  Hoyer, C ^a   Monajembashi, S ^a   Greulich, K O ^a  

^a IMB Jena   (Germany)

Author keywords

caged compound; DNA; electric field; microbeads; optical tweezers; single molecules

Indexed keywords

ANODES; ELECTRIC FIELD EFFECTS; ENZYMES; IMAGE PROCESSING; IONS; LASER APPLICATIONS; MAGNESIUM; MOLECULES; OPTICAL MICROSCOPY; PHOTOLYSIS; REACTION KINETICS; ULTRAVIOLET RADIATION;

ENDONUCLEASE; MICROBEADS; OPTICAL TWEEZERS;

DNA;

DNA; MAGNESIUM; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA BINDING; ELECTRIC FIELD; FLUORESCENCE; LASER; PHOTOLYSIS; PRIORITY JOURNAL; ULTRAVIOLET RADIATION;

EID: 0030579522     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(96)01593-3     Document Type: Article

References (19)

1. Ashkin, A., Dziedic, J.M. and Yamane, T. (1987) Optical trapping and manipulation of single cells using infrared laser beams. Nature 330, 769-771.
2. Barnes, M.D., Whitten, W.B. and Ramsey, J.M. (1995) Detecting single molecules in liquids. Anal. Chem. 67, 418A-426A.
3. Bensimon, D., Simon, A.J., Croquette, V. and Bensimon, A. (1995) Stretching DNA with a receding meniscus: Experiments and models. Phys. Rev. Lett. 74, 4754-4757.
4. Bustamante, C. (1991) Direct observation and manipulation of single DNA molecules using fluorescence microscopy. Annu. Rev. Biophys. Chem. 20, 415-446.
5. Eigen, M. and Rigler, R. (1994) Sorting single molecules: Application to diagnostics and evolutionary biotechnology. Proc. Natl. Acad. Sci USA, 93, 5740-5747.
6. Endlich, N., Harim, A. and Greulich, K.O. (1994) Microdissection of single DNA molecules and DNA-polycation complexes with a UV laser microbeam in a classical light microscope. Exp. Tech. Phys. 40, 87-93.
7. Endlich, N. and Greulich, K.O. (1995) Observation and manipulation of different structural variants of individual cation-DNA complexes in the light microscope. J. Biotechnol. 41, 149-153.
8. Endlich, N., Hoyer, C., Harim, A., Monajembashi, S. and Greulich, K.O. (1996) Micromanipulation of single DNA molecules by laser microbeam and optical tweezers. Exp. Tech. Phys. 41, 303-311.
9. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K. and Yanagida, T. (1995) Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 374, 555-559.
10. Goodwin, P.M., Schecker, J.A., Wilkerson, C.W., Hammond, M.L., Ambrose, W.P., Jett, J.H., Marrone, B.L. and Keller, R.A. (1993) DNA sequencing by single molecule detection of labelled nucleotides sequentially cleaved from a single strand of DNA. Advances in DNA Sequencing Technology. SPIE Vol. 1891, 127-131.
11. Greulich, K.O., Bauder, U., Monajembashi, S., Ponelies, N., Seeger, S. and Wolfrum, J. (1989) Laser microbeam and optical tweezers. Labor 2000, 36-42 (in german).
12. Greulich, K.O., Bauer, E., Fiedler, U., Hoyer, C., König, K. and Monajembashi, S. (1995) Single cell and single molecule laser biotechnology. Progress in Biomedical Optics. SPIE 2629, 62-69.
13. Perkins, T.T., Smith, D.E., Larson, R.G. and Chu, S. (1995) Stretching of a single tethered polymer in a uniform flow. Science 268, 83-87.
14. Ponelies, N., Scheef, J., Harim, A., Leitz, G. and Greulich, K.O. (1994) Laser micromanipulators for biotechnology and genome research. J. Biotechnol. 35, 109-120.
15. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning of double-stranded cDNA, Homopolymeric tailing. In: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, Vol. 2 (second edition) p.8.21.
16. Smith, S.B., Cui, Y. and Bustamante, C. (1996) Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules. Science 71, 795-799.
17. Wang, Y.K., Huff, E.J. and Schwartz, D.C. (1995) Optical mapping of site-directed cleavages on single DNA molecules by the RecA-assisted restriction endonuclease technique. Proc. Natl. Acad. Sci. USA 92, 165-169.
18. Weber, G. and Greulich, K.O. (1992) Manipulation of cells, organelles and genomes by laser microbeam and optical trap. Int. Rev. Cytol. 133, 1-41.
19. Xue, Q. and Young, E.S. (1995) Differences in the chemical reactivity of individual molecules of an enzyme. Nature 373, 681-683.