New synthetic method of 5-formyluracil-containing oligonucleotides and their melting behavior

Tetrahedron Letters

Volumn 37, Issue 50, 1996, Pages 9067-9070

  Sugiyama, Hiroshi ^a   Matsuda, Shigeo ^a   Kino, Katsuhito ^a   Zhang, Qiu Mei ^a   Yonei, Shuji ^a   Saito, Isao ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGOMER; OLIGONUCLEOTIDE; PERIODATE SODIUM; URACIL;

ARTICLE; CHEMICAL REACTION; DRUG SYNTHESIS; OXIDATION; REACTION ANALYSIS;

EID: 0030577491     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(96)02130-2     Document Type: Article

References (11)

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2. 2. Bjelland, S.; Eide, L.; Time, R. W.; Stole, R.; Eftedal, I.; Volden, G.; Seeberg, E. Biochemistry, 1995, 34, 14758-14764.
3. 3. Saito, I.; Takayama, M.; Kawanishi, S. J. Am. Chem. Soc. 1995, 117, 5590-5591.
4. 4. Bjelland, S.; Birkeland, N.-K.; Benneche, T.; Volden, G.; Seeberg, E. J. Biol. Chem. 1994, 269, 30489-30495.
5. 5. Zhang, Q. -M.; Fujimoto, J.; Yonei, S. Int. J. Radiat. Biol. 1995, 68, 603-607.
6. 6. (a) Ono, A.; Okamoto, T.; Inada, M.; Nara, H.; Matsuda, A. Chem. Pharm. Bull. 1994, 42, 2231-2237.
7. (b) Matusda, A.; Inada, M.; Nara, H.; Ohtsuka, E.; Ono, A. Bio. Med. Chem. Lett., 1993, 3, 2751-2754.
8. 7. Rahim, S. G.; Duggan, M. J. H.; Walker, R. T.; Jones, A. S.; Dyer, R. L.; Balzarini, J.; de Clercq, E. Nucleic Acids Res. 1982, 10, 5285-5295.
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11. 10. A variety of 5-foU-containing oligonucleotides including 22 mer for enzymatic incorporation experiment was prepared. All coupling yields, including that with 8 were higher than 97%. Generally, more than 20 OD of purified oligomer was obtained in a 1 μmol scale synthesis.