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Strictly, the convergence on cAMP is not equal: SCPs can elevate cAMP much higher than MMs can (9, 18, 22). However, what matters for our purposes here is that the downstream effects of the SCPs and MMs on the Ca current (18) (Fig. 3A) and the relaxation rate (Fig. 3C) are equal, probably because only relatively small increases in cAMP are important: for example, they suffice to enhance the Ca current maximally (18).
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In addition to somewhat different experimental protocols (29), major variability in this work is due to differences between animals and especially between batches of animals used in different seasons or years. Differences between batches can be much larger than those within each batch. This can be a serious problem in a study such as this that correlates a variety of data sets. As a result, for instance, we cannot be sure that the deviations of the experimental data in Fig. 4 from the model predictions are not simply the result of somewhat different responses of the animals used in the experiments and those used to obtain the data on which the model was based. This provides one justification for keeping the modeling simple and focused only on major features.
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note
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-5 M (13).
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note
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I (15, 18) (Fig. 3, A and B, shows a subset of the same data). The FRFs are assumed not to modulate the Ca current (8). For normalization of the data, see (30).
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note
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B").
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B, which lie somewhat more above the surface.
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note
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We thank E. C. Cropper and B. Bank for the unpublished data included in Fig. 2A, and E. C. Cropper, M. L. Scott, C. Erxleben, and I. Kupfermann for comments on the manuscript. This work was funded by NIH grants MH36730, GM320099 (K.R.W.), and K21 MH00987 (V.B.) and the Whitehall Foundation (V.B.).
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