Hypertension induced in pregnant mice by placental renin and maternal angiotensinogen

Science

Volumn 274, Issue 5289, 1996, Pages 995-998

  Takimoto, Eriko ^a   Ishida, Junji ^a   Sugiyama, Fumihiro ^a   Horiguchi, Hisashi ^a   Murakami, Kazuo ^a   Fukamizu, Akiyoshi ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGY; BODY FLUIDS; GENES; PHYSIOLOGY; PROTEINS; TISSUE;

ANGIOTENSINOGEN; BLOOD PRESSURE; HISTOPATHOLOGY; HYPERTENSION; MICE; MYOCARDIAL HYPERTROPHY; PATHOGENESIS; PATHOPHYSIOLOGY; PLACENTAL RENIN; PREGNANCY;

BIOLOGICAL MATERIALS;

ANGIOTENSINOGEN; MESSENGER RNA; RENIN;

ADOLESCENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EDEMA; FEMALE; FETUS; GLOMERULUS; HEART MUSCLE NECROSIS; HEART VENTRICLE HYPERTROPHY; HISTOPATHOLOGY; MALE; MATERNAL HYPERTENSION; MOUSE; NONHUMAN; PLACENTA; PREGNANCY COMPLICATION; PRIORITY JOURNAL; PROTEIN URINE LEVEL; RENIN ANGIOTENSIN ALDOSTERONE SYSTEM; RENIN RELEASE; TRANSGENIC MOUSE;

ANGIOTENSIN II; ANGIOTENSINOGEN; ANIMALS; BLOOD PRESSURE; CARDIOMEGALY; CROSSES, GENETIC; DISEASE MODELS, ANIMAL; FEMALE; HUMANS; HYPERTENSION; KIDNEY GLOMERULUS; MALE; MICE; MICE, TRANSGENIC; PLACENTA; PREGNANCY; PREGNANCY COMPLICATIONS, CARDIOVASCULAR; RENIN;

ANIMALIA; GLOMERULUS; MUS MUSCULUS;

EID: 0030575910     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5289.995     Document Type: Article

References (28)

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21. The systolic blood pressure of pregnant mice was measured by a programmable sphygmomanometer (BP-98; Sftron, Japan) by using the tailcuff method [K. Tanimoto et al., J. Biol. Chem. 269, 31334 (1994)]. Transgenic mice and age-matched wildtype mice (C57BL/6J) at 2 to 4 months were used for cross-mating.
22. Human active renin concentration was measured by direct radioimmunoassay (8). Total renin concentration was defined as the renin concentration measured after prorenin activation by trypsin (Sigma) [T. Lenz, J. E. Sealey, P. August, G. D. James, J. H. Laragh, J. Clin. Endocrinol. Metab. 69, 31 (1989)]. Inactive renin concentration was calculated as the difference between active and total renin concentration. Mouse renin did not cross-react with the human monoclonal antibodies used in this assay.
23. All animals were placed in individual metabolic cages (CLEA Japan Inc.) with free access to food and water. After an adaptation period of 1 week, 24-hour urine samples were collected. Total urinary protein concentration was measured by the protein assay kit (Bio-Rad). Transgenic mice and wild-type mice at 4 months of age were used for cross-mating.
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26. Placentas of wild-type mice mated with male mice carrying the human renin gene were fixed in 4% paraformaldehyde, embedded in paraffin, and cut into 3-μm serial sections. Sections were deparaffinized and hybridized with a digoxigenin-labeled probe, synthesized as sense and antisense RNAs from the Aat I-Sac I 405-base pair fragment of the human renin cDNA (13) in the presence of digoxigenin-labeled uridine-triphosphate (Boehringer Mannheim). After the color reaction, the sections were counter stained with methyl green.
27. Tissues of pregnant mice were fixed in 10% buffered formalin and embedded in paraffin. Deparaffinized tissue sections were stained with hematoxylin and eosin, and other reagents such as periodic acid-Schiff and Masson trichrome if necessary.
28. We thank the Laboratory Animal Research Center at the University of Tsukuba and T. Watanabe and T. Mori for their technical advice and assistance. Care of experimental animals was within institutional guidelines. Supported by grants from the Ministry of Education, Science, Sports and Culture of Japan and Circulation Biosystems at the University of Tsukuba. E.T. and J.I. are reseach fellows of the Japan Society for the Promotion of Science.