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15
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85030268219
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note
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10. Lipase from porcine pancreas (PPL, Type-II) was purchased from Sigma Chemical Co (USA).
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16
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0011861953
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Ed. Drauz, K.; Waldmann, H., VCH Publishers: Weinheim, Germany
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11. Waldmann, H. Enzyme Catalysis in Organic Synthesis. Vol. II; Ed. Drauz, K.; Waldmann, H., VCH Publishers: Weinheim, Germany, 1995; pp.868.
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Waldmann, H.1
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85030274204
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note
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12. Microemulsions containing lipase were prepared by stirring a mixture of PPL (0.25g) and reverse micellar solution of isooctane-water - AOT [sodium bis-(2-ethylhexyl) sulphosuccinate], which in turn was prepared by shaking a mixture of water (4ml), isooctane (25ml) and AOT (4.9 g). Gelatin (7g) was dissolved in water (10ml) preheated to 50°C and the above microemulsion containing enzyme was added to it with shaking while maintaining the temperature a little above 50°C, the gel transition temperature. The resulting microemulsion-based gels (MBGs) were subsequently cooled to room temperature and pelleted prior to use.
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18
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85030274285
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note
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1H NMR spectra of all the sixteen compounds exhibited the characteristic J values; from the EIMS and IR spectral data of these compounds, only the prominent and characteristic peaks are included).
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0011907064
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14. Lakshmi, C.; Raj, N.G.; Srinivasan, K.S.V.C.; Kumar, K.A. Chem. Abstr. 1989, 110, 153838.
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Lakshmi, C.1
Raj, N.G.2
Srinivasan, K.S.V.C.3
Kumar, K.A.4
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85030269245
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note
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16. The isolated yields of the products are much lower than the % of conversions of the substrates as it was quite difficult to remove gelatin from the reaction mixtures and several chromatographic operations had to be performed, followed by prep TLC/ crystallization to isolate the products in the pure form, we have not recovered the substrates. No by-product was observed to be formed during the course of these reactions.
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