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1 receptor filtration binding assay was performed similarly to the one described in reference 2 (a). Details on the specific of our assays will be found in the following paper; Greig G. M.; Dufresne, C.; Evans J.; Favreau, L.; Francis D.; Gallant, M.; Gareau, Y.; Kargman S.; Labelle, M.; Metters, K. M.; Rochette C.; Ruel R.; Sawyer N.; Slipetz, D. M.; Tremblay N.; Weech P. K.; O'Neill, G. P. Expression and ligand binding characterization of the recombinant human central and peripheral cannabinoid receptors; submitted
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25. It was found that the N1 acylation of indole using an acid chloride as the acyl source was greately improved if the indole potassium salt (KHMDS) was used in presence of HMPA instead of the usual sodium salt.
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