Site-directed mutagenesis of human prostacyclin synthase: Alteration of Cys441 of the Cys-pocket, and Glu347 and Arg350 of the EXXR motif

FEBS Letters

Volumn 389, Issue 3, 1996, Pages 268-272

  Hatae, Toshihisa ^a   Hara, Shuntaro ^a   Yokoyama, Chieko ^a   Yabuki, Tomoko ^a   Inoue, Hiroyasu ^a   Ullrich, Volker ^b   Tanabe, Tadashi ^a  

^a NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Cytochrome P450; Prostacyclin synthase; Prostaglandin I2; Site directed mutagenesis

Indexed keywords

6 OXOPROSTAGLANDIN F1 ALPHA; ARGININE; CYSTEINE; GLUTAMINE; PROSTACYCLIN; PROSTACYCLIN SYNTHASE; PROSTAGLANDIN H2;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; EXPRESSION VECTOR; HUMAN; IMMUNOBLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

EID: 0030575194     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(96)00600-X     Document Type: Article

References (34)

1. [1] Moncada, S. and Vane, J.R. (1979) N. Engl. J. Med. 300, 1142-1147.
2. [2] Moncada, S. and Vane, J.R. (1979) Pharmacol. Rev. 30, 293-331.
3. [3] Ullrich. V., Castle, L. and Weber, P. (1981) Biochem. Pharmacol. 30, 2033-2036.
4. [4] Graf, H., Ruf, H.H. and Ullrich, V. (1983) Angew. Chem. 95, 497-498.
5. [5] Graf, H., Ruf, H.H. and Ullrich, V. (1983) Angew. Chem. Int. Ed. Engl. 22, 487-488.
6. [6] Hara, S., Miyata, A., Yokoyama, C., Inoue, H., Brugger, R., Lottspeich, F., Ullrich, V. and Tanabe, T. (1994) J. Biol. Chem. 269, 19897-19903.
7. [7] Miyata, A., Hara, S., Yokoyama, C., Inoue, H., Ullrich, V. and Tanabe, T. (1994) Biochem. Biophys. Res. Commun. 200, 1728-1734.
8. [8] Tanabe, T. and Ullrich, V. (1995) J. Lipid Mediators Cell Signal. 12, 243-255.
9. [9] Hasemann, C.A., Kurumbail, R.G., Boddupalli, S.S., Peterson, J.A. and Deisenhofer, J. (1995) Structure 2, 41-62.
10. [10] Zvelebil, M.J.J.M., Wolf, C.R. and Sternberg, M.J.E. (1991) Protein Eng. 4, 271-282.
11. [11] Yokoyama, C., Wang, X., Briggs, M., Admon, A., Wu, J., Hua, X., Goldstein, J. and Brown, M. (1993) Cell 75, 187-197.
12. [12] Innis, M.A., Gelfand, D.H. and Sninsky, J.J. (1995) PCR Strategies, Academic Press, San Diego, CA.
13. [13] Akusjävi, G., Svensson, C. and Nygard, O. (1987) Mol. Cell. Biol. 7, 549-551.
14. [14] Yokoyama, C., Miyata, A., Suzuki, K., Nishikawa, Y., Yoshimoto, T., Yamamoto, S., Nüsing, R., Ullrich, V. and Tanabe, T. (1993) FEBS Lett. 318, 91-94.
15. [15] Siegle, I., Nüsing, R., Brugger, R., Sprenger, R., Zecher, R. and Ullrich, V. (1994) FEBS Lett. 347, 221-225.
16. [16] DeWitt, D.L. and Smith, W.L (1983) J. Biol. Chem. 258, 3285-3293.
17. [17] Poulos, T.L., Finzel, B.C., Gunselus, I.C., Wagner, G.C. and Kraut, J. (1985) J. Biol. Chem. 260, 16122-16130.
18. [18] Nelson, D.R. and Strobel, H.W. (1988) J. Biol. Chem. 263, 6038-6056.
19. [19] Nebert, D.W. and Gonzalez, F.J. (1987) Annu. Rev. Biochem. 56, 945-993.
20. [20] Shimizu, T., Hirano, K., Takahashi, M. and Hatano, M. (1988) Biochemistry 27, 4138-4141.
21. [21] Xia, Z., Shen, R.F., Baek, S.J. and Tai, H.H. (1993) Biochem. J. 295, 457-461.
22. [22] Tomura, D., Sai, G., Endo, M., Kudo, T. and Shoun, H. (1995) Seikagaku 67, 592 (in Japanese).
23. [23] Yoshikawa, K., Noguti, T., Tsujimura, M., Koga, H., Yasukochi, T., Horiuchi, T. and Gô, M. (1992) Biochim. Biophys. Acta 1122, 41-44.
24. [24] Chen, S. and Zhou, D. (1992) J. Biol. Chem. 267, 22587-22594.
25. [25] Shimizu, T. Tateishi, T., Hatano, M. and Fujii-Kuriyama, Y. (1991) J. Biol. Chem. 266, 3372-3375.
26. [26] Noshiro, M. and Okuda, K. (1990) FEBS Lett. 268, 137-140.
27. [27] Yokoyama, C., Miyata, A., Ihara, H., Ullrich, V. and Tanabe, T. (1991) Biochem. Biophys. Res. Commun. 178, 1479-1484.
28. [28] Kawajiri, K., Gotoh, O., Sogawa, K., Tagashira, Y., Muramatsu, M. and Fujii-Kuriyama, Y. (1984) Proc. Natl. Acad. Sci. USA 81, 1649-1653.
29. [29] Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama. S., Fujii-Kuriyama, Y. and Omura, T. (1987) J. Biol. Chem. 262, 1706-1711.
30. [30] Haniu, M., Armes, L.G., Tanaka, M., Yasunobu, K.T., Shastry, B.S., Wagner, G.C. and Gunsalus, I.C. (1982) Biochem. Biophys. Res. Commun. 105, 889-894.
31. [31] Haniu, M., Armes, L.G., Yasunobu, K.T., Shastry, B.S. and Gunsalus, I.C. (1982) J. Biol. Chem. 257, 12664-12671.
32. [32] Peterson, J.A, Lu, J.Y. Geisselsoder, J., Losence, S.G., Carmona, C., Witney, F. and Lorence, M.C. (1992) J. Biol. Chem. 267, 14193-14203.
33. [33] Narhi, L.O. and Fulco, A.J. (1986) J. Biol. Chem. 261, 7160-7169.
34. [34] Kizawa, H., Tomura, D., Oda, M., Fukamizu, A., Hoshino, T., Gotoh, O., Yasui, T. and Shoun, H. (1991) J. Biol. Chem. 266, 10632-10637.