A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50- to 100-kb genomic fragments for direct DNA sequencing

Gene

Volumn 179, Issue 1, 1996, Pages 181-188

  Wild, Jadwiga ^a   Hradečná, Zdenka ^a   Pósfai, György ^a   Szybalski, Waclaw ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Bypassing conventional cloning; DNA fragments generated directly from chromosomes of living cells; Escherichia coli; oriV; Plasmid RK2; SPEL 6 sequencing by primer walking; Yeast FRT Flp excision system

Indexed keywords

DNA FRAGMENT;

ARTICLE; BACTERIOPHAGE; BACTERIUM TRANSDUCTION; DNA SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPRESSOR GENE;

CHROMOSOME WALKING; CLONING, MOLECULAR; DNA NUCLEOTIDYLTRANSFERASES; ESCHERICHIA COLI; GENOME, BACTERIAL; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PLASMIDS; RECOMBINATION, GENETIC; REPLICATION ORIGIN; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030574265     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00487-8     Document Type: Article

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