TR146 cells as a model for human buccal epithelium: II. Optimisation and use of a cellular sensitivity MTS/PMS assay

International Journal of Pharmaceutics

Volumn 141, Issue 1-2, 1996, Pages 217-225

  Jacobsen, Jette ^a   Pedersen, Morten ^b   Rassing, Margrethe Rømer ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b Cheminova AS   (Denmark)

Author keywords

Cell line TR146; Cellular drug sensitivity MTS PMS assay; Human buccal epithelium; Morphine and morphine prodrugs; Adrenoceptor antagonists

Indexed keywords

ATENOLOL; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; METOPROLOL; MORPHINE; MORPHINE DERIVATIVE; OXPRENOLOL; PRODRUG; PROPRANOLOL;

ARTICLE; BIOLOGICAL MODEL; CELL DENSITY; CHEEK MUCOSA; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG SENSITIVITY; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL;

EID: 0030572689     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-5173(96)04640-6     Document Type: Article

References (28)

1. Altman, F.P., Tetrazolium salts and formazans. Prog. Histochem. Cytochem., 9 (1977) 1-56.
2. Barsuhn, C.L., Olanoff, L.S., Gleason, D.D., Adkins, E.L. and Ho, N.F.H., Human buccal absorption of flurbiprofen. Clin. Pharmacol. Ther., 44 (1988) 225-231.
3. Beckett, A.H. and Triggs, E.J., Buccal absorption of basic drugs and its application as an in vivo model of passive drug transfer through lipid membranes. J. Pharm. Pharmacol., 19 (1967) 31S-41S.
4. Burgalassi, S., Panichi, L., Saettone, M.F., Jacobsen, J., Rassing, M.R., Development and in vitro in vivo testing of mucoadhesive buccal patches releasing benzydamine and lidocaine. International Journal of Pharmaceutics, 133 (1996) 1-7.
5. Buttke, T.M., McCubrey, J.A. and Owen, T.C., Use of an aqueous soluble tetrazolium formazan assay to measure viability and proliferation of lymphokine-dependent cell lines. J. Immunol. Methods, 157 (1993) 233-240.
6. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D. and Mitchell, J.B., Evaluation of a tetrazolium-bazed semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res., 47 (1987a) 936-942.
7. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D. and Mitchell, J.B., Evaluation of a tetrazolium-bazed semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res., 47 (1987b) 943-946.
8. Chen, T.R., In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res., 104 (1977) 255-262.
9. Drustrup, J., Fullerton, A., Christrup, L. and Bundgaard, H., Utilisation of prodrugs to enhance the transdermal absorption of morphine. Int. J. Pharm., 71 (1991) 105-116.
10. Hansen, L.B., Christrup, L.L. and Bundgaard. H., Enhanced delivery of ketobemidone through porcine buccal mucosa in vitro via more lipophilic ester prodrugs. Int. J. Pharm., 88 (1992) 237-242.
11. Hansen, M.B., Nielsen, S.E. and Berg, K., Re-examination and further development of a precise and rapid dye method for measuring cell growth cell kill. J. Immunol. Methods, 119 (1989) 203-210.
12. Harris, D. and Robinson, J.R., Drug delivery via the mucous membranes of the oral cavity. J. Pharm. Sci., 81 (1992) 1-10.
13. Jacobsen, J., van Deurs, B., Pedersen, M. and Rassing, M.R., TR146 cells grown on filters as a model for human buccal epithelium. I.: Morphology, growth, barrier properties, and permeability. Int. J. Pharm., 125 (1995) 165-184.
14. Komiya, I., Park. J.Y., Kamani, A., Ho, N.F.H. and Higuchi, W.I., Quantitative mechanistic studies in simultaneous fluid flow and intestinal absorption using steroids as model solutes. Int. J. Pharm., 4 (1980) 249-262.
15. Le Brun, P.P.H., Fox, P.L.A., de Vries, M.E. and Boddé, H.E., In vitro penetration of some β-adrenoreceptor blocking drugs through porcine buccal mucosa. Int. J. Pharm., 49 (1989) 141-145.
16. Lind, K., Mycoplasmer. In Kielberg, V., Brünner, N. and Briand, P. (Eds), Celledyrkning. En praktisk håndbog i dyrkning af mammale feller. FADL., Hvidovre, 1994, p. 71.
17. Loveland, B.E., Johns, T.G., Mackay, I.R., Vaillant, F., Wang, Z.-X. and Herzog, P.J., Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays. Biochem. Int., 27 (1992) 501-510.
18. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65 (1983) 55-63.
19. Pimlott, S.J. and Addy, M., Evaluation of a method to study the uptake of prednisolone sodium phosphate from different oral mucosal sites. Oral Surg. Oral Med. Oral Pathol., 60 (1985) 35-37.
20. Plumb, J.A., Milroy, R. and Kaye, S.B., Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay. Cancer Res., 49 (1989) 4435-4440.
21. Promega Corporation, Tech. Bull. No. 169, 1992.
22. Rathbone, M.J. and Hadgraft, J., Absorption of drugs from the human oral cavity. Int. J. Pharm., 74 (1991) 9-24.
23. Roehm, N.W., Rodgers, G.H., Hatfield, S.M. and Glasebrook, A.L., An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. Immunol. Methods, 142 (1991) 257-265.
24. Rupniak, T.H., Rowlatt, C., Lane, E.B., Steele, J.G., Trejdosiewicz, L.K., Laskiewicz, B., Povey, S. and Hill, B.T., Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. J. Natl. Cancer Inst., 75 (1985) 621-635.
25. Scudiero, D.A., Shoemaker, R.H., Paull, K.D., Monks, A., Tierney, S., Nofziger, T.H., Currens, M.J., Seniff, D. and Boyd, M.R., Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 48 (1988) 4827-4833.
26. Stevens, M.G. and Olsen, S.C., Comparative analysis of using MTT and XTT in colorimetric assays for quantitating bovine neutrophil bactericidal activity. J. Immunol. Methods, 157 (1993) 225-231.
27. Tavakoli-Saberi, M.R. and Audus, K.L., Physicochemical factors affecting β-adrenergic antagonist permeation across cultured hamster pouch buccal epithelium. Int. J. Pharm., 56 (1989) 135-142.
28. Zheng, H. and Audus, K.L., Cytotoxic effects of chlorhexidine and nystatin on cultured hamster buccal epithelial cells. Int. J. Pharm., 101 (1994) 121-126.