Analysis of yeast trimethylguanosine-capped RNAs by Midwestern blotting

Gene

Volumn 182, Issue 1-2, 1996, Pages 89-96

  Rasmussen, Theodore P ^a   Culbertson, Michael R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Antibody; Caps; Immuno detection; snoRNA; snRNA

Indexed keywords

ANTIBODY; ANTISENSE OLIGONUCLEOTIDE; CAPPED RNA; FUNGAL RNA; GUANOSINE DERIVATIVE; METHYL GROUP; NYLON; SMALL NUCLEAR RNA;

ARTICLE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST;

ANTIBODIES; BLOTTING, NORTHERN; DNA PROBES; DNA, ANTISENSE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GUANOSINE; IMMUNOBLOTTING; RNA CAPS; RNA, FUNGAL; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0030571552     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00519-7     Document Type: Article

References (45)

1. Andersen, J. and Zieve, G.W. (1991) Assembly and intracellular transport of snRNP particles. Bioessays 13, 57-64.
2. Bachellerie, J.P., Michot, B., Nicoloso, M., Balakin, A., Ni, J. and Fournier, M.J. (1995) Antisense snoRNAs: a family of nucleolar RNAs with long complementarities to rRNA. Trends Biochem. Sci. 20, 261-264.
3. Balakin, A.G., Schneider, G.S., Corbett, M.S., Ni, J. and Fournier, M.J. (1993) SnR31, snR32, and snR33: three novel, non-essential snRNAs from Saccharomyces cerevisiae. Nucleic Acids Res. 21, 5391-5397.
4. Bochnig, P., Reuter, R., Bringmann, P. and Luhrmann, R. (1987) A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs. Eur. J. Biochem. 168, 461-467.
5. Bonen, L. (1993) Trans-splicing of pre-mRNA in plants, animals, and protists. FASEB 7, 40-46.
6. Bringmann, P., Reuter, R., Rinke, J., Appel, B., Bald, R. and Luhrmann, R. (1983a) 5′-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs. J. Biol. Chem. 258, 2745-2747.
7. Bringmann, P., Rinke, J., Appel, B., Reuter, R. and Luhrmann, R. (1983b) Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera. EMBO J. 2, 1129-1135.
8. Devereux, J., Jaeberli, P. and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12, 387-395.
9. England, T.H. and Uhlenbech, O.C. (1978) 3′-Terminal labelling of RNA with T4 RNA ligase. Nature 275, 560-561.
10. Fournier, M.J. and Maxwell, E.S. (1993) The nucleolar snRNAs: catching up with the spliceosomal snRNAs. Trends. Biochem. Sci. 18, 131-135.
11. Gershoni, J.M. and Palade, G.E. (1982) Electrophoretic transfer of proteins from sodium dodecy sulfate-polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 124, 396-405.
12. Gershoni, J.M. and Palade, G.E. (1983) Protein blotting: principles and applications. Anal. Biochem. 131, 1-15.
13. Guthrie, C. (1991) Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein. Science 253, 157-163.
14. Hamm, J., Darzynkiewicz, E., Tahara, S.M. and Mattaj, I.W. (1990) The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal. Cell 62, 569-577.
15. Hashimoto, C. and Steitz, J.A. (1986) A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro. Cell 45, 581-591.
16. Henriques, R., Blobel, G. and Aris, J.P. (1990) Isolation and sequencing of NOP1. J. Biol. Chem. 265, 2209-2215.
17. Hughes, J.M.X., Konings, D.A.M., and Cesareni, G. (1987) The yeast homolog of U3 snRNA. EMBO J. 6, 2145-2155.
18. Karwan, R. and Kindas Mugge, I. (1989) Identification of a yeast ribonuclease H as an Sm antigen. Eur. J. Biochem. 179, 549-555.
19. Krainer, A.R. (1988) Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs. Nucleic Acids Res. 16, 9415-9429.
20. Lamond, A.I. (1993) The spliceosome, Bioessays 15, 595-603.
21. Leeds, P., Peltz, S.W., Jacobson, A. and Culbertson, M.R. (1991) The product of the yeast UPF1 gene is required for rapid turnover of mRNAs containing a premature translational termination codon. Genes Dev. 5, 2303-2314.
22. Li, Z. and Brow, D.A. (1993) A rapid quantitative detection of specific RNAs. Nucleic Acids Res. 21, 5645-4646.
23. Liu, M.H., Busch, R.K., Buckley, B. and Reddy, R. (1992) Characterization of antibodies against methyl-pppN cap structure: plant U3 small nucleolar RNA is recognized by these antibodies. Nucleic Acids Res. 20, 4299-4304.
24. Mattaj, I.W., Tollervey, D. and Seraphin, B. (1993) Small nuclear RNAs in messenger RNA and ribosomal RNA processing. FASEB 7, 47-53.
25. Maxwell, E.S. and Fournier, M.J. (1995) The small nucleolar RNAs. Ann. Rev. Biochem. 35, 897-934.
26. Melin, L., Soldati, D., Mital, R., Streit, A. and Schumperli, D. (1992) Biochemical demonstration of complex formation of historic premRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors. EMBO J. 11, 691-697.
27. Mowry, K.L. and Steitz, J.A. (1987) Identification of the human U7 snRNP as one of several factors involved in the 3′ end maturation of histone premessenger RNA's. Science 238, 1682-1687.
28. Munns, T.W., Liszewski, J.T., Tellam, H.F., Sims, H.F., and Rhoads, R.E. (1982) Antibody-nucleic acid complexes. Immunospecific retention of blobin messenger ribinucleic acid with antibodies specific for 7-methylguanosine. Biochemistry 21, 2922-2928.
29. Okano, Y. and Medsger, T.A., Jr. (1992) Novel human autoantibodies reactive with 5′-terminal trimethylguanosine cap structures of U small nuclear RNA. J. Immunol. 149, 1093-1098.
30. Parker, R., Simmons, T., Shuster, E.O., Siliciano, P.G. and Guthrie, C. (1988) Genetic analysis of small nuclear RNAs in Saccharomyces cerevisiae: viable sextuple mutant. Mol. Cell. Biol. 8, 3150-3159.
31. Patterson, B. and Guthrie, C. (1987) An essential yeast snRNA with a U5-like domain is required for splicing in vivo. Cell 49, 613-624.
32. Plessel, G., Fischer, U. and Luhrmann, R. (1994) m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain. Mol. Cell. Biol. 14, 4160-4172.
33. Riedel, N., Wise, J.A., Swerdlow, H., Mak, A. and Guthrie, C. (1986) Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity. Proc. Natl. Acad. Sci. 83, 8097-8101.
34. Rymond, B.C. (1993) Convergent transcripts of the yeast PRP38-SMD1 locus encode two essential splicing factors, including the D1 core polypeptide of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. 90, 848-852.
35. Shimba, S., Buckley, B., Reddy, R., Kiss, T. and Filipowicz, W. (1992) Cap structure of U3 small nucleolar RNA in animal and plant cells is different: gamma-monomethyl phosphate cap structure in plant RNA. J. Biol. Chem. 267, 13772-13777.
36. Singh, R. and Reddy, R. (1989) Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA. Proc. Natl. Acad. Sci. 86, 8280-283.
37. Smith, J.H. and Eliceiri, G.L. (1983) Antibodies elicited by N2,N2-7-trimethylguanosine react with small nuclear RNAs and ribonucleoproteins. J. Biol. Chem. 258, 4636-4640.
38. Spycher, C., Streit, A., Stefanovic, B., Albrecht, D., Koning, T.H. and Schumperli, D. (1994) 3′ end processing of mouse histone premRNA: evidence for additional base-pairing between U7 snRNA and pre-mRNA. Nucleic Acids Res. 22, 4023-4030.
39. 3G capped RNAs by immunoblotting. Nucleic Acids Res. 22, 252-253.
40. Steitz, J.A. and Tycowski, K.T. (1995) Small RNA chaperones for ribosome biogenesis. Science 270, 1626-1627.
41. Terns, M.P. and Dahlberg, J.E. (1994) Retention and 5′ cap trimethylation of U3 snRNA in the nucleus. Science 264, 959-961.
42. Terns, M.P., Grimm, C., Lund, E. and Dahlberg, J.E. (1995) A common maturation pathway for small nucleolar RNAs. EMBO J. 14, 4860-4871.
43. Tollervey, D. (1987) High level of complexity of small nuclear RNAs in fungi and plants. J. Mol. Biol. 196, 355-361.
44. Tyr gene are both transcribed in a HeLa cell extract but spliced along different pathways. EMBO J. 6, 35-41.
45. Wise, J.A., Tollervey, D., Maloney, D., Swerdlow, H., Dunn, E.J. and Guthrie, C. (1983) Yeast contains small nuclear RNAs encoded by single copy genes. Cell 35, 743-751.