A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: Production of the human T cell receptor Vβ5.3 in Escherichia coli

Gene

Volumn 182, Issue 1-2, 1996, Pages 101-109

  Andrews, Beth ^a   Adari, Hedy ^a   Hannig, Gerhard ^a   Lahue, Elaine ^a   Gosselin, Michael ^a   Martin, Sue ^a   Ahmed, Asma ^a   Ford, Pamella J ^a   Hayman, Edward G ^a   Makrides, Savvas C ^a  

^a T Cell Sciences Inc   (United States)

Author keywords

Expression plasmid; IPTG; lacI(q)ts; lacIts; Recombinant DNA

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; T LYMPHOCYTE RECEPTOR; TETRACYCLINE;

ARTICLE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE REPRESSION; HEAT SENSITIVITY; HUMAN; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; T LYMPHOCYTE RECEPTOR GENE; TRANSCRIPTION INITIATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION; GENES, BACTERIAL; GENES, VIRAL; GENETIC MARKERS; GENETIC VECTORS; HUMANS; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECEPTORS, ANTIGEN, T-CELL; REPRESSOR PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030571551     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00523-9     Document Type: Article

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